101,530 research outputs found
Data for: Chemical structure of hydrolysates of cereulide and their time course profile
No full textThe data provided in this article supplements the data information provided in “Chemical structure of hydrolysates of cereulide and their time course profile”. The generation of the data considered synthesis procedure of depsipeptides and elucidation of natural products using HPLC-TOFMS. Hydrolysates of cereulide which is an emetic toxin were found in the broth of Bacillus cereus. Two tetradepsipeptides and two dodecadepsipeptides which were cleaved ester bond of cereulide, were synthesized using liquid phase fragment condensation method starting from commercially available amino acids. The chemical structure of hydrolysates was verified tetradepsipeptide L-O-Val-L-Val-D-O-Leu-D-Ala and dodecadepsipeptide (D-O-Leu-D-Ala-L-O-Val-L-Val)3 using LC-TOFMS. Quantitative analysis revealed the time course profile of cereulide production and degradation. Production in the stationary phase and decomposition in the death phase of cereulide were revealed. The increase in tetradepsipeptide continued after the stationary phase until decomposition occurred
Data for: Chemical structure of hydrolysates of cereulide and their time course profile
No full textThe data provided in this article supplements the data information provided in “Chemical structure of hydrolysates of cereulide and their time course profile”. The generation of the data considered synthesis procedure of depsipeptides and elucidation of natural products using HPLC-TOFMS. Hydrolysates of cereulide which is an emetic toxin were found in the broth of Bacillus cereus. Two tetradepsipeptides and two dodecadepsipeptides which were cleaved ester bond of cereulide, were synthesized using liquid phase fragment condensation method starting from commercially available amino acids. The chemical structure of hydrolysates was verified tetradepsipeptide L-O-Val-L-Val-D-O-Leu-D-Ala and dodecadepsipeptide (D-O-Leu-D-Ala-L-O-Val-L-Val)3 using LC-TOFMS. Quantitative analysis revealed the time course profile of cereulide production and degradation. Production in the stationary phase and decomposition in the death phase of cereulide were revealed. The increase in tetradepsipeptide continued after the stationary phase until decomposition occurred
Aphaenogaster gamagumayaa Naka & Maruyama 2018, sp. nov.
No full textAphaenogaster gamagumayaa Naka & Maruyama, sp. nov. Type series. Holotype, worker, Nakagusuku-son, Okinawa-jima, Japan, 10 IX 2017, T. Naka (The Institute of Tropical Agriculture, Kyushu University = KUM, no MMANT001). Paratypes, 7 workers, the same locality, collected between 31 VIII - 10 IX 2017 (5 in KUM, nos. MMANT002-006; 2 in MCZC = Museum of Comparative Zoology, Harvard University, Cambridge, MA, USA, nos. MMANT007,008). Diagnosis. This species is distinguished from the other East Asian species by having the most elongate body, the longest antennae and legs, and the most reduced eyes. Among the Japanese species, it is most similar to A. irrigua Watanabe & Yamane, 1999 described from Ryukyu Archipelago. It differs from A. irrigua in lighter color, smaller eyes (EL 0.19 x TmL vs. 0.38 x TmL), basal margin of mandible with weaker serration, and scapes more elongate and slim (SL 2.28 x HW vs. 1.53 x HW). Measurements. Workers (n = 6). HL, 1.499 ± 0.047 (1.439–1.566); HW, 1.118 ± 0.030 (1.073–1.152); TmL, 0.790±0.038 (0.743–0.843); GL, 0.808±0.027 (0.778–0.856); SL, 2.429±0.053 (2.350–2.509); EL, 0.143±0.006 (0.134–0.149); EW, 0.119±0.005 (0.114–0.128); ML, 2.563±0.074 (2.463–2.659); PSL, 0.363±0.017 (0.348– 0.388); SDL, 0.252±0.010 (0.242–0.270); HTL, 2.121±0.047 (2.046–2.176); PL, 0.753±0.026 (0.702–0.769); PPL, 0.480±0.017 (0.465–0.513); PH, 0.389±0.008 (0.380–0.399); PPH, 0.356±0.034 (0.330–0.423); PNW, 0.748±0.022 (0.713-0.775); PW, 0.271±0.009 (0.260–0.283); PPW, 0.327±0.007 (0.317–0.338). Description. Body (Figs. 1–4) almost entirely yellowish. Head and mesosoma yellow, but mandibles and antennae darker, and base of head and anterior area of prothorax brown; legs light yellow but bases of femora, tibiae and tarsi darker. Gaster light yellow, but basal constriction brown, and posterior 1/2 slightly darker. Head (Figs. 2, 3) oval, without basal constriction or neck. Anterior margin of clypeus with weak transverse wrinkles and shallowly concave. Eyes very small, 0.19 times as long as length of tempora. Scapes elongate and slim, 2.28 times as long as width of head, at base 1.7 times as wide as at apex, gradually widened, straight, only apex slightly bent down with slight preapical constriction. Surface of scape shiny, uniformly covered with short and sparse adherent setae. Scape straight, only apex slightly bent down with shallow preapical constriction. Funicle elongate and thin, 1.38 times as long as scape, first funicular segment elongate, 3.17 times as long as wide at apex, 1.74 times as long as second segment, relative lengths of segments, 100:57:68:75:76:76:92:150:145:143:213, apical segment 3.1 times as wide as first segment. Pronotum (Figs.1, 4) elongate, 1.37 times as long as wide, regularly convex in profile. Propodeum (Figs. 1, 4) almost as long as wide, propodeal spines short, needle-like, obliquely directed upwards. Petiole (Fig. 1,4) elongate with long peduncle, its anterior face deeply concave, node globular and strongly convex. Ventral margin of petiole with low, short carina around middle. In dorsal view, petiole gently widened postriorly before petiolar node. Postpetiole (Figs. 1, 4) short, 0.68 times as long as petiole, in profile regularly rounded, its node slightly lower than petiole. In dorsal view postpetiole 1.5 times as long as wide with regularly rounded sides. Mandible (Fig. 3) elongate, with outer edge straight, dorsal surface with distinct striation, inner margin with 7 or 8 small teeth. Lateral portion of clypeus (Fig. 3) with 2–3 thin oblique rugae, central part without sculpture, shiny. Frontal carinae short, not extending to line connecting anterior margin of eyes, subparallel; interantennal area deeply impressed, smooth and shiny, frontal triangle shiny, with a few, shallow longitudinal wrinkles. Anterior portion of frons with thin longitudinal rugae, mesal area between eyes glabrous and shiny, posterior portion around vertex without rugae but with distinct microreticulation (Fig. 3). Pronotum (Figs. 1, 4) with microreticulation, but with smooth areas postero-laterally, with 8 short setae. Top of mesonotum (Figs. 1, 4) covered with strong longitudinal rugae; mesopleuron with distinct granulate sculpture, matte. Propodeum (Figs. 1, 4) with slightly granulate sculpture, below spiracles with two short, thin, longitudinal rugae with distinct transverse wrinkles, and dorsal surface of both mesonotum and propodeum appears slightly matte. Entire petiole and postpetiole covered with fine microreticulation, without rugae. appearing slightly matte, covered with several sparse setae. Gaster (Fig. 1) smooth, shiny, without microreticulation except in basal area, tergites with sparse, suberect setae much shorter than propodeal spines. Legs (Fig. 1) very long, hind femora 1.13 times as long as mesosoma, hind tibiae 0.77 times as long as hind femora, hind tarsi 1.19 times as long as hind femora. Surface of legs shiny, fore tarsi only on ventral surface covered with very short, appressed pubescence; femora and mid- and hind tibiae completely without pubescence. Queens and males are unknown. Etymology. The specific epithet is a Ryukyuan dialect “ gamagumayaa ” (= cave-dwelling hermit), referring to the habitat of the new species. Biological notes. The type series of Aphaenogaster gamagumayaa is based on workers probably from a single nest, collected in a limestone cave on the island of Okinawa. All specimens were found in a guano hall (Fig. 5), an area of approximately 25 m 2 (2–3 m in height), approximately 20 m from the cave entrance. The hall is completely dark, and during the study period (August to October 2017), it was consistently cooler (<25°C during the day) than the exterior of the cave (28–32°C). The cave contains no pools or streams but is generally wet, and the substrate is clay soil. The ants were moving around an approximately 4-m2 area in which there were many small holes (Fig. 6) and cracks in the cave floor. Ants were observed entering these holes. Although one of the authors, TN, did not dig into the holes, they were surmised to be associated with a core part of the nest. TN observed a maximum of 12 individual workers, most likely nest mates; no aggression was seen between them. Most of them were solitary foragers and observed on the cave floor or on the lower part of the cave wall. However, on one occasion two ants were observed walking together (Fig. 7) for some time. On two occasions ants were seen carrying balls of guano into a hole (Fig. 8). Another individual was observed carrying a small white object that did not appear to be guano. Upon perceiving human movement, the ants stopped moving and hid. While motionless, they waved their antennae, most likely to assess the situation. Gaster bending behavior, which is often observed in other Aphaenogaster species (Terayama et al. 2014), has not been observed in A. gamagumayaa.Published as part of Naka, Takeru & Maruyama, Munetoshi, 2018, Aphaenogaster gamagumayaa sp. nov.: the first troglobiotic ant from Japan (Hymenoptera: Formicidae: Myrmicinae), pp. 135-141 in Zootaxa 4450 (1) on pages 136-139, DOI: 10.11646/zootaxa.4450.1.10, http://zenodo.org/record/144455
House in Ushita Naka
No full text[ES] Arquitectos / Architects: Kazunori Fujimoto Architect & Associates. Kazunori Fujimoto (principal responsable / principal-in-charge), Tomoyasu Hogaki. · Localización / Location: Higashi-ku, Hiroshima, Japan · Redacción de proyecto / Project Planning: 2004 · Período de construcción / Construction term: 2005 · Superficie de la parcela / Site area: 100.1 m2 · Superficie del edificio / Building area: 109.2 m2 · Ocupación en planta / Total floor area: 60 m2 · Ingeniero estructural / Structural engineer: Ike Structural Consultants · Dirección de obra / Construction Team: Alf Co.,Ltd. · Fotografía / Photography: Kazunori FujimotoFujimoto, K.; Hogaki, T. (2017). Casa en Ushita Naka. EN BLANCO. Revista de Arquitectura. 9(22):44-51. https://doi.org/10.4995/eb.2017.7600SWORD445192
Magnetic susceptibility and electrical resistivity of a mesoporous carbon CMK-1
No full textThe magnetic susceptibility of the title material follows a Curie-Weiss law at high temperatures, and a peak of the susceptibility appears around 4 K associated with remarkable hysteresis below 10 K, which is reminescent of a spin-glass behavior. The temperature. dependence of the electrical resistivity is proportional to exp (T-1/3), which may reveal a two-dimensional variable range hopping
Letter, [Author unclear] to Paulina T. Merritt
No full textHandwritten letter to Paulina Merritt from an unknown author, October 1, 1876.
Information sharing between process and engineering design activity in CAD environment
No full textRecently chemical process industry has focused on information technology that can treat various types of information through lifecycle activities. In this study, facility model is proposed to share information between process and engineering design activity. Facility model is supported by feature and, parametric approach to deal with attribute and geometrical information that happens in the design activities. And a prototype based on CAD environment is shown as an integrated information management system
Expression of NCC, NaKa, and MR in kidney homogenates from wild-type (WT) and Vpr transgenic (Tg) mice.
No full text(A) Protein expression of total NCC was markedly suppressed in kidneys from Tg mice compared with WT littermates. n = 6. **P t test. (B) Protein expressions of NaKa and MR were similar in WT and Tg mice. NaKa, Na+/K+-ATPase. n = 3. n.s., not significant, Student’s t test. (C) Immunohistochemical analysis of NCC distribution in kidney cortex sections demonstrated that NCC is located in the apical cellular regions of the renal distal convoluted tubule, with similar distribution in both WT and Tg mice. NCC immunostained tubules were scanned and NCC protein expression was found to be lower in Tg than WT mice both before and after salt depletion. n = 3–4. n.s., not significant; **P P < 0.001, ANOVA, Bonferroni correction.</p
Handwritten biographical information on Paulina T. McClung Merritt
No full textA handwritten biography of Paulina T. McClung Merritt by an unknown author, 1892.
Heterogeneous and tissue-specific regulation of effector T cell responses by IFN-gamma during Plasmodium berghei ANKA infection.
No full textIFN-γ and T cells are both required for the development of experimental cerebral malaria during Plasmodium berghei ANKA infection. Surprisingly, however, the role of IFN-γ in shaping the effector CD4(+) and CD8(+) T cell response during this infection has not been examined in detail. To address this, we have compared the effector T cell responses in wild-type and IFN-γ(-/-) mice during P. berghei ANKA infection. The expansion of splenic CD4(+) and CD8(+) T cells during P. berghei ANKA infection was unaffected by the absence of IFN-γ, but the contraction phase of the T cell response was significantly attenuated. Splenic T cell activation and effector function were essentially normal in IFN-γ(-/-) mice; however, the migration to, and accumulation of, effector CD4(+) and CD8(+) T cells in the lung, liver, and brain was altered in IFN-γ(-/-) mice. Interestingly, activation and accumulation of T cells in various nonlymphoid organs was differently affected by lack of IFN-γ, suggesting that IFN-γ influences T cell effector function to varying levels in different anatomical locations. Importantly, control of splenic T cell numbers during P. berghei ANKA infection depended on active IFN-γ-dependent environmental signals--leading to T cell apoptosis--rather than upon intrinsic alterations in T cell programming. To our knowledge, this is the first study to fully investigate the role of IFN-γ in modulating T cell function during P. berghei ANKA infection and reveals that IFN-γ is required for efficient contraction of the pool of activated T cells
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