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Analisi di composti organici volatili (VOC) di specie di papilionideae caratteristiche dell’areale mediterraneo
No full textComposition of volatile in micropropagated and field grown aromatic plants from Tuscany Islands
No full textAromatic plant species present in the natural Park of Tuscany Archipelago are used as flavoring agents and spices, as dietary supplements and in cosmetics and aromatherapy. The plants are usually collected in wild field, inducing a depauperization of the natural habitat. Therefore, micropropagation of these aromatic plants can play a role for the protection of the natural ecosystem, can guarantee a massive production and can provide standardized plant materials for further economical purposes. The aim of this study is to compare the volatile organic compounds produced from the wild plants with the in vitro plantlets using headspace solid phase micro-extraction (HS-SPME), followed by capillary gas-chromatography coupled to mass spectrometry (GC-MS). Typical plants of this natural area selected for this work were Calamintha nepeta L., Crithmum maritimum L., Lavandula angustifolia L., Myrtus communis L., Rosmarinus officinalis L., Salvia officinalis L., Satureja hortensis L. Different explants have been used microcuttings with vegetative apical parts, axillary buds or internodes. Sterilization percentage, multiplication rate and shoot length, as well as root formation were measured. The volatile aromatic profiles produced from in vitro plantlets were correlated to that of the wild plants, in particular for C. maritimum, R. officinalis, S. officinalis and S. hortensis. This study indicated that the micropropagation technique can represent a valid alternative to produce massive and steril plant material characterised by the same aromatic flavour than the wild grown plant
Estradiol-antagonistic Activity of Phenolic Compounds from Leguminous Plants
No full textNatural flavonoids are currently receiving much attention because of their estrogenic and antiestrogenic properties. Six isoflavones (isoprunetin, isoprunetin 7-O-beta-D-glucopyranoside, isoprunetin 4',7-di-O-beta-D-glucopyranoside, genistein, genistein 7-O-beta-D-glucopyranoside, daidzein), four flavones (luteolin, luteolin 7-O-beta-D-glucopyranoside, luteolin 4'-O-beta-D-glucopyranoside, licoflavone C), isolated from Genista morisii and G. ephedroides (two Leguminosae plants of the Mediterranean area) together with two structurally related pterocarpans, bitucarpin A and erybraedyn C, isolated from Bituminaria bituminosa (Leguminosae), were tested for the antagonist activity by a yeast based estrogen receptor assay (Saccharomyces cerevisiae RMY326 ER-ERE). Most compounds inhibited the estradiol-induced transcriptional activity in a concentration dependent manner. In particular, for the flavone luteolin 77% inhibition of the induced beta-galactosidase activity was observed. Interestingly, licoflavone C exhibited a dose-dependent antagonistic activity at concentrations up to 10(-4) M, but stimulated beta-galactosidase expression at higher concentrations resulting in a U-shaped-like dose-response curve
In vitro cultures of Bituminaria bituminosa: pterocarpan, furanocoumarin and isoflavone production and cytotoxic activity evaluation
No full textBituminaria bituminosa L. is known for producing several compounds with considerable pharmaceutical interest, such as phenylpropanoids, furanocoumarins and pterocarpans. In vitro cultures of seedlings, shoots, and callus have been produced to obtain plant materials useful for the production of these metabolites. The secondary metabolite profile was evaluated by HPLC-DAD. The extracts of all the in vitro material contained the flavonoid daidzein, while plicatin B, erybraedin C and bitucarpin A were found only in the extracts of the in vitro shoots and in wild shoots. The furanocoumarins angelicin and psoralen were found in in vivo and in vitro plants, but in the callus were not detectable. The extracts were also tested for cytotoxic activity in HeLa cell culture; the highest level of cytotoxicity was found in in vitro shoot extracts
Volatile constituents as complementary tools to characterize seven sardinian Genista species
No full textThe aroma fingerprints of seven endemic Sardinian populations of Genista species (Genistabocchierii, Genista arbusensis, Genista cadasonensis, Genista corsica, Genista morisii, Genista pichisermolliana, Genista sulcitana) were defined for the first time in order to investigate the relationship between their chemotaxonomic classification and discriminating volatiles. It is the first time that the inter-species compositional variations of non-terpenoidic constituents and target volatiles such as linalool, limonene, 2,6-nonadienal, 2-pentylfuran, (E)-2-(2-pentenyl)-furan, ß-caryophyllene and caryophyllene oxide were related by Principal Components Analysis to the actual chemotaxonomic classification of the selected Sardinian Genista species. Although they are not considered marketable aromatic plants, these original results have shown the potential role of volatiles not only as new further important chemotaxonomic markers of Genista species, but also as potential ecological factors in the chemodiversity of some endemic Mediterranean Genista or, more in general, in vegetation changes and reforestation processes
“ ESTROGEN ANTAGONIST ACTIVITY OF SOME PHYTOCHEMICALS FROM LEGUMINOSAE PLANTS OF THE MEDITERRANEAN AREA”
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