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[Anemia in primary hyperparathyroidism. Description of 2 case].
No full textTwo cases of hyporigenerative anaemia in primary hyperparathyroidism are reported. The absence of other causes of anaemia and the correction of hematological disorder after parathyroidectomy, indicates that PTH hypersecretion is responsible for anaemia. The relationships between calcium levels controlling mechanisms and erithropoiesis are discussed
Analysis of apoptosis by propidium iodide staining and flow cytometry
No full textSince its introduction, the propidium iodide (PI) flow cytometric assay has been widely used for the evaluation of apoptosis in different experimental models. It is based on the principle that apoptotic cells, among other typical features, are characterized by DNA fragmentation and, consequently, loss of nuclear DNA content. Use of a fluorochrome, such as PI, that is capable of binding and labeling DNA makes it possible to obtain a rapid (the protocol can be completed in about 2 h) and precise evaluation of cellular DNA content by flow cytometric analysis, and subsequent identification of hypodiploid cells. The original protocol enhanced the capacity for a rapid, quantitative measure of cell apoptosis. For this reason, since its publication, the PI assay has been widely used, as demonstrated by the large number of citations of the original paper and/or the continuous use of the method in many laboratorie
Zeolin is a recombinant storage protein with different solubility and stability properties according to its localization in the endoplasmic reticulum or in the chloroplast.
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