4,968 research outputs found
Motivi letterari nei libri per l’infanzia di Paolo Di Paolo
No full textThe article, organised in the form of a discussion, aims to analyse the themes and motifs of Paolo Di Paolo’s works that are addressed to young audiences. The author has recently published a large number of volumes directed just at younger readers. When beholding the titles that constitute the author’s bibliography, the reader is struck by Di Paolo’s predisposition to transform literary classics: as much in Giacomo il signor bambino as in the edition of the Divina Commedia, the author’s goal is to try to reach his new readers. Therefore, Di Paolo proves to be a prolific author of the genre; in fact, in his bibliography, volumes of fairy tales with a classic slant, such as La mucca volante, are listed as well.L’articolo contiene un’analisi di temi e di motivi delle opere di Paolo Di Paolo dirette al pubblico più piccolo. L’autore negli ultimi anni ha pubblicato un cospicuo numero di volumi indirizzati, infatti, proprio ai lettori più giovani. Ciò che stupisce scorgendo i titoli che costituiscono la bibliografia dell’autore, è la predisposizione a trasformare i classici della letteratura: tanto in Giacomo il signor bambino quanto nell’edizione della Divina Commedia l’obiettivo è cercare di raggiungere i lettori più giovani. Di Paolo si dimostra, quindi, un autore prolifico del genere, tant’è che nell’elenco non mancano volumi fiabeschi dal taglio classico come La mucca volante. Analizzare le opere giovanili dello scrittore costituisce un fatto inedito
Signaling and transcriptomics at the degenerating-regenerating neuromuscular junction
No full textThe neuromuscular junction (NMJ) is a specialized tripartite synapse that allows the transmission of an electrical impulse travelling along the axon to the muscle. It is composed of the motor axon terminal (MAT), covered by perisynaptic Schwann cells (PSCs), and the muscle fibre (MF), which are separated by a basal lamina. The NMJ is not protected by anatomical barriers: it can be therefore exposed to traumas, to the attack of many pathogens including neurotoxins, and affected by many neuromuscular diseases such as amyotrophic lateral sclerosis and immune-mediated disorders, such as the Guillain-Barré and Miller Fisher syndromes. For these reasons and for its essential role in life and survival the NMJ has retained throughout vertebrate evolution an intrinsic ability for repair and regeneration, differently from central synapses. After nerve injury the glial cells of the NMJ, the PSCs, acquire a regenerative phenotype and release a series of factors that act on the stump of the MAT, providing several cues to promote neuronal regeneration. Following peripheral nerve injury, many changes taking place at the NMJ have been reported so far, but the inter- and intra-cellular signaling that occur during MAT degeneration and, more importantly, those governing the ensuing regeneration are not completely understood.
We have recently established a model to study NMJ degeneration and regeneration in mice based on the specific action of -latrotoxin, a presynaptic neurotoxin isolated from the venom of the black widow spider, which targets specifically the presynaptic terminal causing its complete degeneration. Following intoxication and the subsequent clearing of MAT debris by PSCs, the axon stump regrows in few days in mice allowing complete NMJ recovery. This toxin represents therefore a simple and controlled method to induce an acute, localized and reversible nerve terminal degeneration not blurred by inflammation, and can help to identify molecules involved in the intra- and inter-cellular signalling governing NMJ regeneration.
In the search of candidate molecules involved in triggering and sustaining nerve recovery we choose to perform a transcriptomic analysis of the mouse NMJs at different time points after injection of -latrotoxin. This approach has been very challenging: to our knowledge a transcriptomic analysis of the sole NMJ was never reported before. We succeeded in collecting a number of NMJs suitable for RNA isolation and sequencing of both coding and non–coding RNAs. Among the coding transcripts we selected a series of messenger RNAs (mRNAs) that are expressed at low level in controls, at higher levels during regeneration, and then return to basal when substantial regeneration is attained and we selected the mRNA encoding for the chemokine CXCL12. We found that CXCL12 is produced by PSCs during nerve degeneration, and that intraperitoneal injection of a neutralizing antibody for CXCL12 slows down the regeneration process. Moreover, the exposure of primary motor neurons to the recombinant chemokine stimulates neurite growth.
These data suggest that CXCL12 is an important factor released by PSCs with a crucial role in the nerve terminal regeneration process.
Parallely, we looked for molecules released by injured neurons that could activate SCs and stimulate nerve regeneration. We found that ATP released by intoxicated neurons activates a series of intracellullar signaling pathways in SCs including Ca2+, adenylate cyclase, ERK 1/2 and CREB, that are of fundamental importance for the recovery of nerve function. We propose ATP as an important alarm signal partecipating in the cross-talk between degenerating nerve terminals and adjacent PSCs not only in a model of degeneration by a spider toxin, but also in different forms of neurodegeneration of the presynaptic nerve terminal
Informe preliminar del rescate del sitio Gallucci departamente de El Cuy (Pcia. de Río Negro)
No full textFil: Béguelin, Marien. Universidad Nacional de Río Negro. Río Negro, Argentina.Fil: Vazquez, Romina Clara. Universidad Nacional de Río Negro. Río Negro, Argentina.Fil: Otero, Feliipe. Universidad Nacional de Río Negro. Río Negro, Argentina.Fil: Citton, Paolo. Universidad Nacional de Río Negro. Río Negro, Argentina.Fil: Caselli, Alberto Tomás. Universidad Nacional de Río Negro. Río Negro, Argentina.Fil: Otero, Estanislada. Universidad Nacional de Río Negro. Río Negro, Argentina.En el presente informe se indican las actividades vinculadas al trabajo de rescate arqueológico
desarrolladas en la margen sur del río Negro a la altura de Fernández Oro, el día 23 de octubre de 2020.
Se exhumaron los restos óseos humanos hallados y denunciados por el Sr. Cristian Denis S. Gallucci el
día 12 de octubre del mismo año.
A modo de reconocimiento por la acción del denunciante, con la cual se favorece el cuidado, la
protección y el estudio del patrimonio provincial, el equipo decidió nombrar al lugar del hallazgo “Sitio
Gallucci”.
Participaron de este trabajo:
Coordinación y Organización General: Marien Béguelin
Excavación y registro: Romina Vazquez (Dirección); Felipe Otero; Paolo Citton; Estanislada Otero;
Alberto Caselli
Las tareas fueron solicitadas por la Secretaría de Cultura de la Provincia de Río Negro luego de que el
Cuerpo de Investigación Forense de la Cuarta Circunscripción Judicial -Cipolletti- determinara que el
hallazgo no revestía interés forense (Pericia N°A-4CI-709-CIF2020) y se realizaron siguiendo lo
previsto por la Ley Provincial Nº 3041/03
Proceedings of EAEE WG15 Summer Workshop
No full textThe Summer Workshop of the European Association of Earthquake Engineering (EAEE) Working Group 15 (WG15) was intended to provide a forum for the ad hoc assembly of knowledge to produce State-of-the-Art papers for dissemination at the European level. Based on the reported activities of the WG15 members and
their collaborators, visions have been developed based on promising integrated techniques for environmental refurbishment and seismic retrofitting. The state-of-the-art results collected have been compared with the outcomes from each WG sub-group and will be used to develop a technical position paper for the scientific community, practitioners, and policymakers. The position paper will provide a comprehensive overview of the state-of-the-art results, identify key gaps in the current research, and make recommendations for future research and policy decisions. The contribution of the experts will ensure that the position paper is based on
current evidence and best practices. More than 30 experts have contributed their work to the workshop. This was ultimately successful in creating an understanding of the current research conducted in different seismic-prone regions, as well as potential areas of future development. The proceedings include the presentations and final discussion from the workshop
Chronic migraine plus medication overuse headache. two entities or not?
No full textChronic migraine (CM) represents migraine natural evolution from its episodic form. It is realized through a chronicization phase that may require months or years and varies from patient to patient. The transition to more frequent attacks pattern is influenced by lifestyle, life events, comorbid conditions and personal genetic terrain, and it often leads to acute drugs overuse. Medication overuse headache (MOH) may complicate every type of headache and all the drugs employed for headache treatment can cause MOH. The first step in the management of CM complicated by medication overuse must be the withdrawal of the overused drugs and a detoxification treatment. The goal is not only to detoxify the patient and stop the chronic headache but also to improve responsiveness to acute or prophylactic drugs. Different methods have been suggested: gradual or abrupt withdrawal; home treatment, hospitalization, or a day-hospital setting; re-prophylaxes performed immediately or at the end of the wash-out period. Up to now, only topiramate and local injection of onabotulinumtoxinA have shown efficacy as therapeutic agents for re-prophylaxis after detoxification in patients with CM with and without medication overuse. Although the two treatments showed similar efficacy, onabotulinumtoxinA is associated with a better adverse events profile. Recently, the Phase III Research Evaluating Migraine Prophylaxis Therapy (PREEMPT) clinical program proved that patients with CM, even those with MOH, are the ones most likely to benefit from onabotulinumtoxinA treatment. Furthermore, it provided an injection paradigm that can be used as a guide for a correct administration of onabotulinumtoxinA
- …
