20,123 research outputs found
SDS-PAGE gel showing the expression levels of PRC1-DD WT, mutant PRC1-DD-N+4 and PRC1-DD-N+His.
No full textEach lane shows the eluted protein profile with induction time labeled on top of each lane. The expression system in E.Coli/Rosseta (DE3). The molecular weight of PRC1-DD is around 15kD. (PDF)</p
DD-Akt(E40K) is inducible in a neuronal context.
No full text(A) Western immunoblot analysis of DD-Akt(E40K) induction by TMP in neuronal PC12 cells. Neuronal PC12 cells were infected as indicated with a lentiviral vector expressing either eGFP alone (pWPI) or the DD-Akt(E40K) fusion gene and eGFP. Four days post-infection, cells were treated with or without 10 μM TMP as indicated for 24 hr and then harvested for western immunoblotting. Blots were probed as indicated for total Akt, pAkt(pT308), pAkt(pS473) and GAPDH (loading control). A representative blot is shown. (B) Average fold induction of DD-Akt forms by TMP treatment in neuronal PC12 cultures. N = 4–6 independent experiments with duplicate or triplicate wells per condition. Graph shows means with SEM. *p ≤ 0.05, paired t-test, +TMP vs.–TMP levels. (C) The relative expressions of induced DD-pAkt(E40K) and endogenous pAkt levels in neuronal PC12 cells quantified and calculated as a percent ratio. (D) Western immunoblot analysis of total and phospho-S473 forms of DD-Akt(E40K) induction by TMP in cultured rat cortical neurons. Cultured rat cortical neurons were infected as indicated with a lentiviral vector expressing either eGFP alone (pWPI) or the DD-Akt(E40K) fusion gene and eGFP. Four days post-infection, cells were treated with or without 10 μM TMP as indicated for 24 hr and then harvested for western immunoblotting. Blots were probed as indicated for total Akt, pAktT308, pAktS473 and GAPDH (loading control). Assessment of GFP expression indicated an infection efficiency of about 50% of the neurons. A representative blot is shown. (E). Average fold induction of total and DD-pAkt(E40K-pS473) by TMP treatment in cortical neuron cultures. N = 2 samples per condition. Values are means with SEM. *p<0.05, **p<0.01, unpaired t-test, +TMP vs -TMP.</p
DD-Akt(E40K) induction is dose-responsive to TMP treatment and reversible.
No full text(A) Western immunoblotting analysis of dose-response of DD-Akt(E40K) induction by TMP. HEK293 cells were transfected with eGFP-only expressing vector (EV) or DD-Akt(E40K) fusion protein expression vector and treated with indicated doses of TMP for 24 hr before analysis by western immunoblotting with the indicated probes. (B) Quantification of DD-Akt(E40K) induction (as determined by western immunoblotting) by various doses of TMP. Values means with SEM. N = 3–4 independent experiments. (C) Time course of DD-Akt(E40K) induction by TMP. HEK293 cells were transfected with DD-Akt(E40K) expression vector, treated with 10 μM TMP for the indicated times and then assessed for total DD-Akt(E40K) levels by western immunoblotting as in (A). Values are means with SEM. N = 2–4 independent experiments. (D) Reversal of DD-Akt(E40K) induction after TMP washout. HEK293 cultures transfected with DD-Akt(E40K) as above were treated with 10 μM TMP for 24 hr, washed free of TMP and harvested for western immunoblotting at the indicated time points. Total DD-Akt(E40K) levels were determined by western immunoblotting as in (A). Data shown are from one experiment.</p
Račun opservabli nt – nt, dd – pt i dd – dd raspršenja primjenom realističnih međudjelovanja
No full textThe four-body equations of Alt, Grassberger and Sandhas are solved for a system of four nucleons, using realistic NN interactions in channels 1S0, 3S1−3D1, 1P1, 3P0, 3P1 and 3P2. The results of the calculation are compared with data for the reactions dd → dd, dd → p 3H and n3H → n 3H. The calculations indicate that the nucleon-nucleon p-waves have a strong effect on 4N observables, but one finds some disagreement with data that indicates the need for a 3N force or new 2N+3N force models.Rješavaju se jednadžbe Alta, Grassbergera i Sandhasa za četiri tijela za sustav četiri nukleona uz primjenu realističnih pretpostavki o NN-međudjelovanju u kanalima 1S0, 3S1−3D1, 1P1, 3P0, 3P1 i 3P2. Ishodi računa uspoređuju se s podacima za reakcije dd → dd, dd → p 3H i n3H → n 3H. Računi pokazuju da nukleon – nukleon p-valovi snažno utječu na 4N opservable, ali neka neslaganja s podacima ukazuju na mogućnost pogreške u poznavanju stvarnih međudjelovanja
Račun opservabli nt – nt, dd – pt i dd – dd raspršenja primjenom realističnih međudjelovanja
No full textThe four-body equations of Alt, Grassberger and Sandhas are solved for a system of four nucleons, using realistic NN interactions in channels 1S0, 3S1−3D1, 1P1, 3P0, 3P1 and 3P2. The results of the calculation are compared with data for the reactions dd → dd, dd → p 3H and n3H → n 3H. The calculations indicate that the nucleon-nucleon p-waves have a strong effect on 4N observables, but one finds some disagreement with data that indicates the need for a 3N force or new 2N+3N force models.Rješavaju se jednadžbe Alta, Grassbergera i Sandhasa za četiri tijela za sustav četiri nukleona uz primjenu realističnih pretpostavki o NN-međudjelovanju u kanalima 1S0, 3S1−3D1, 1P1, 3P0, 3P1 i 3P2. Ishodi računa uspoređuju se s podacima za reakcije dd → dd, dd → p 3H i n3H → n 3H. Računi pokazuju da nukleon – nukleon p-valovi snažno utječu na 4N opservable, ali neka neslaganja s podacima ukazuju na mogućnost pogreške u poznavanju stvarnih međudjelovanja
Regulating GDNF expression using DD.
No full text<p>(A) Histogram showing the levels of GDNF produced from DD-YFP vector, wild type GDNF, GDNF-DD and DD-GDNF, as measured by GDNF ELISA. C-terminal fusion of the DD to GDNF did not result in any GDNF production, while N-terminal DD-GDNF fusion resulted in TMP-induced GDNF production. Furthermore, the DD-GDNF was functional in that it induced tyrosine hydroxylase expression in the TGW cell line as shown in (B).</p
Global Compilation of Published Modern Sedimentary Wax dD
No full textDatabase of dD values of published sedimentary plant n-alkanes and n-acids, with dD of OIPC-modeled annual precipitation and dD of lake water where availabl
The Influence of Intellectual/Developmental Disabilities (I/DD) Curriculum Content on Career Interests for Social Work Students
No full textThis study examines how curriculum content on Intellectual/Developmental Disabilities (I/DD) impacts the career interests of social work students. With the growing demand for social workers proficient in I/DD services, it is crucial to understand how educational experiences shape career choices. Using a QUANT→Qual mixed-method approach, this quasi-experimental pre/post-test survey combined quantitative data (N 66 [experimental group]; N =103 [control group] and open-ended survey questions (N = 66 [experimental group]; N =103 [control group] from social work students.
The author-constructed survey assessed student attitudes, perceptions, preparedness, ability to identify I/DD, knowledge of I/DD, and willingness to practice with individuals with I/DD before and after exposure to I/DD-focused curriculum content over a 15-week period. The quantitative findings indicated that exposure to I/DD-focused curriculum content significantly improved students’ preparedness, ability to identify I/DD, knowledge about I/DD, and willingness to work with this population. Qualitative findings indicated that students who completed the I/DD courses reported increased confidence and interest in pursuing careers involving I/DD roles. The study findings emphasize the importance of including dedicated I/DD content in social work education to better equip future practitioners
Clinical Management of Pregnant Transplant Recipients: Utilization of Fetal Fraction and dd-cfDNA
No full textPurpose: One of many benefits of kidney transplant (KT) is restoration of fertility in women allowing the option to conceive. However, pregnancy in this population poses significant maternal and fetal challenges. Tools to accurately monitor for allograft rejection during pregnancy are non-specific and biopsy of the allograft can in some cases lead to unintended complications. Immunosuppression management is also complicated due to vast physiologic changes during pregnancy. Utilization of fetal and donor-derived cell-free DNA (dd-cfDNA) may provide better insights for management. Methods: This is a prospective, multi-center, observational study of KT patients undergoing dd-cfDNA (AlloSure, CareDx) monitoring monthly during pregnancy. All KT patients undergo dd-cfDNA surveillance as standard of care. The samples were interrogated for different genomes to quantify fetal fraction (FF) and ddcfDNA. The Kruskal-Wallis test was performed to analyze the dd-cfDNA and FF across time points. Results: Total of 7 KT recipients were included in this analysis. Of those, 5 had live births, 1 had a miscarriage, and 1 is ongoing. Complications during pregnancy included pre-eclampsia (2), post-partum bleeding (2), and allograft rejection (1). One patient was treated for clinical rejection during pregnancy due to rise in serum creatinine (1.3 to 1.7), however, the correlating dd-cfDNA remained stable at 0.23% indicating lack of rejection. Overall, FF significantly increased from 1stto 3rdtrimester (p\u3c0.0003 (figure1a)) as expected due to increase in fetal mass. The dd-cfDNA did not differ across 3 trimesters (Figure 1b) indicating lack of injury to kidney allograft. The evolution of FF and dd-cfDNA from pre- to post-pregnancy is described in figure 1c. Clinical parameters correlated with pregnancy related changes in transplant population rather than allograft health (figure 1d). Conclusions: Utilization of dd-cfDNA and fetal fraction provides specific insights into kidney allograft function. This can lead to optimized noninvasive monitoring and management of pregnancy in this patient population. CITATION INFORMATION: Khoury N., Jittirat A., Fu Y., Gulbahce N., Woodward R., Sulejmani N. Clinical Management of Pregnant Transplant Recipients: Utilization of Fetal Fraction and dd-cfDNA AJT, Volume 23, Issue 6, Supplement 1. DISCLOSURES: N.Khoury: n/a. A.Jittirat: n/a. Y.Fu: n/a. N.Gulbahce: Employee;; CareDX. R.Woodward: Employee;; CareDx, Stock Shareholder;; CareDx. N.Sulejmani: Employee;; CareDx. [Figure presented
DD for migratory locust development at Baiquan (BQ), Chengde (CD), Tumotezuoqi (TM), Wenan (WA), Rongan (RA), Qiongzhong (QZ), and Qiongshan (QS) were recalculated by the improved DD model.
No full text<p>The lowercase letter ‘n’ represents the theory generations of <i>L. m. manilensis</i> at each location. Actual DD = Total DD-(Overflow DD + Ineffective DD for egg-laying). Generations completed as follow: (n-1)+[actual DD -700*(n-1)]/700DD.</p
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