123,111 research outputs found
DNA- and RNA-binding ability of oligoDapT, a nucleobase-decorated peptide, for biomedical applications
No full textDomenica Musumeci,1,2 Valentina Roviello,3 Giovanni N Roviello1 1CNR-Institute of Biostructure and Bioimaging, Naples, Italy; 2Department of Chemical Sciences, University of Naples Federico II, Naples, Italy; 3Analytical Chemistry for the Environment and Centro Servizi Metereologici Avanzati, University of Naples Federico II, Naples, Italy Background: Nucleobase-bearing peptides and their interaction with DNA and RNA are an important topic in the development of therapeutic approaches. On one hand, they are highly effective for modulating the nucleic-acid-based biological processes. On the other hand, they permit to overcome some of the main factors limiting the therapeutic efficacy of natural oligonucleotides, such as their rapid degradation by nucleases. Methods and results: This article describes the synthesis and characterization of a novel thymine-bearing nucleoamino acid based on the l-diaminopropionic acid (l-Dap) and its solid phase oligomerization to α-peptides (oligoDapT), characterized using mass spectrometry, spectroscopic techniques, and scanning electron microscopy (SEM) analysis. The interaction of the obtained nucleopeptide with DNA and RNA model systems as both single strands (dA12, rA12, and poly(rA)) and duplex structures (dA12/dT12 and poly(rA)/poly(rU)) was investigated by means of circular dichroism (CD) and ultraviolet (UV) experiments. From the analysis of our data, a clear ability of the nucleopeptide to bind nucleic acids emerged, with oligoDapT being able to form stable complexes with both unpaired and double-stranded DNA and RNA. In particular, dramatic changes in the dA12/dT12 and poly(rA)/poly(rU) structures were observed as a consequence of the nucleopeptide binding. CD titrations revealed that multiple peptide units bound all the examined nucleic acid targets, with TLdap/A or TLdap/A:T(U) ratios >4 in case of oligoDapT/DNA and ~2 in oligoDapT/RNA complexes. Conclusion: Our findings seem to indicate that Dap-based nucleopeptides are interesting nucleic acid binding-tools to be further explored with the aim to efficiently modulate DNA- and RNA-based biological processes. Keywords: nucleopeptides, nucleic acid interaction, poly(rA) binding, circular dichrois
"La fine è nota". Osservazioni a prima lettura alla sentenza n. 162 del 2014 della Corte costituzionale sul divieto di fecondazione eterologa
No full textIl lavoro esamina la complessa motivazione della sentenza n. 162/2014 della Corte costituzione con cui è stata dichiarata l'illegittimità della norma della legge n. 40/2004 che vietava la pratica della fecondazione eterologa in Itali
Amiet (Robert). — Rituale augustanum. Monumenta liturgica ecclesiae augustanae t. XII et XIII, Aoste, Musumeci, 1991
No full textDevos Roger. Amiet (Robert). — Rituale augustanum. Monumenta liturgica ecclesiae augustanae t. XII et XIII, Aoste, Musumeci, 1991. In: Le Monde alpin et rhodanien. Revue régionale d'ethnologie, n°3-4/1993. pp. 141-142
Synthetic approaches to nucleopeptides containing all four nucleobases, and nucleic acid-binding studies on a mixed-sequence nucleo-oligolysine
No full textIn this article we describe two solid-phase synthetic routes to obtain a nucleo-oligolysine α-peptide containing all four natural nucleobases. The first one is based on the oligomerization of the nucleobase-containing monomers, easily synthesized as herein described. The second strategy has the advantage of avoiding the solution synthesis of the monomeric building blocks, leading to the final nucleopeptide by direct solid-phase couplings of the suitably protected nucleobases with the free amino groups on the growing peptide chain still anchored to the resin. Both strategies are general and can be applied to the synthesis of nucleopeptides having backbones formed by any other diamino acid moiety decorated with the four nucleobases. We also report the CD and UV studies on the hybridization properties of the obtained nucleopeptide, containing all four nucleobases on alternate lysines in the sequence, towards complementary DNA and RNA strands. The nucleo-oligolysine with a mixed-base sequence did not prove to bind complementary DNA, but was able to recognize the complementary RNA forming a complex with a higher melting temperature than that of the corresponding RNA/RNA natural duplex and comparable with that of the analogous PNA/RNA system
Christillin (J.JJ. — Légendes et récits recueillis sur les bords du Lys. Aoste, Editions Musumeci, 1970
No full textJoisten Charles. Christillin (J.JJ. — Légendes et récits recueillis sur les bords du Lys. Aoste, Editions Musumeci, 1970. In: Le Monde alpin et rhodanien. Revue régionale d'ethnologie, n°1/1973. p. 100
Synthesis and supramolecular assembly of 1,3-bis(1′-uracilyl)-2- propanone
No full textSIRE(opens in a new window)|View at Publisher|
Export
| Download | Add to List | More...
RSC Advances
Volume 4, Issue 54, 2014, Pages 28691-28698
Synthesis and supramolecular assembly of 1,3-bis(1′-uracilyl)-2- propanone (Article)
Roviello, G.N.a , Roviello, G.b, Musumeci, D.c, Capasso, D.d, Di Gaetano, S.a, Costanzo, M.d, Pedone, C.d
a Istituto di Biostrutture e Bioimmagini-CNR, Via Mezzocannone 16, 80134 Napoli, Italy
b Department of Science and Technology, University of Naples Parthenope, Centro Direzionale, 80143 Napoli, Italy
c Department of Chemical Science, University of Naples Federico II, Via Cinthia, 80126 Napoli, Italy
View additional affiliations
View references (26)
Abstract
In this research work, we investigated the properties of a novel heterocyclic molecule, named by us U2CO, whose structure consists of two uracil rings connected by a 2-propanone moiety. We studied by UV the spectroscopic characteristics of U2CO as a function of temperature and ionic strength of the solution. Furthermore, dynamic light scattering (DLS) studies conducted on samples containing the uracil-containing derivative indicated the formation of non-covalent supramolecular networks based on multiple U2CO units. These aggregates contained hydrophobic cavities able to encapsulate drugs such as doxorubicin, as evidenced by fluorescence studies. This property, together with the good stability in human serum of U2CO, determined by HPLC analysis, could be an interesting feature in developing new drug delivery systems. Interestingly, UV studies suggested that U2CO is able to form complexes with copper(ii) cations, which is a useful characteristic in view of potential anti-oxidant approaches
Il diritto allo studio universitario fra Stato, Regioni e Università. Note a margine al decreto legislativo 29 marzo 2012, n. 68
No full textL'esame dei problemi connessi alla fruibilità del diritto allo studio universitario dopo l'entrata in vigore del d.lgs n. 68 del 201
Recherches sur l'ancienne liturgie d'Aoste et les usages religieux et populaires valdôtains. Aoste, Imp. Marguerettaz-Musumeci, 1959-1971
No full textDevos Roger. Recherches sur l'ancienne liturgie d'Aoste et les usages religieux et populaires valdôtains. Aoste, Imp. Marguerettaz-Musumeci, 1959-1971. In: Le Monde alpin et rhodanien. Revue régionale d'ethnologie, n°1/1973. pp. 99-100
Università e diritto allo studio
No full textIl diritto allo studio universitario ha uno specifico riconoscimento in Costituzione sia in ragione dell'istituzione che lo impartisce, l'Università, sia per la qualificazione culturale dei soggetti che vi accedono, tanto da meritare una qualificazione del tutto diversa dal più generale diritto all'istruzione. Il saggio ricostruisce la struttura e la fruibilità del diritto allo studio universitario dopo la riforma dell'ordinamento universitario posto in essere dalla legge n. 240 del 2010, al fine di dimostrare come l'intrecciato rapporto tra autonomia finanziaria degli atenei e plurimi livelli di governo, Stato e regioni, si stia rivelando sempre più penalizzante per il carattere sociale del diritto
- …
