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Inibitori del "re-uptake" della serotonina nel trattamento dei disturbi dell'umore: sottotipi e comorbidità con altra patologia
DAPI and Confocal Laser-Scanning Microscopy for In Vivo Imaging of Phytoplasmas
As phytoplasmas are located inside the phloem tissue, always surrounded by numerous layers of other cells,
they can result difficult candidates for microscopical investigations. Moreover, the necessity to kill the plant
tissues for microscopy observations causes instantaneous and irreversible modifications in the sieve elements,
leading to misleading information and erroneous interpretations. Phytoplasmas were here investigated
in intact Vicia faba host plants using DAPI as fluorescent probe and confocal laser scanning
microscopy. The described nondestructive technique may be applied for the imaging of phytoplasmas
and of different pathogen-related responses in planta
Phytoplasmas: An Introduction
Phytoplasmas are among the most recently discovered plant pathogens. They are wall-less prokaryotes
restricted to phloem tissue, associated with diseases affecting several hundred plant species. The impact of
phytoplasma diseases on agriculture is impressive and, at the present day, no effective curative strategy has
been developed. The availability of rapid and sensitive techniques for phytoplasma detection as well as the
possibility to study their relationship with the host plants is a prerequisite for the management of
phytoplasma-associated diseases
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