170,148 research outputs found
Preliminary pathological results on chondrodysplastic dwarfism in Tyrolean Grey cattle due to deletion in the EVC2 gene
No full textPreliminary pathological results on chondrodysplastic
dwarfism in Tyrolean Grey cattle due to deletion
in the EVC2 gene
C Benazzi*, KE Dittmer†, KG Thompson†, C Drögemüller‡, A
Gentile*, LV Muscatello*, L Murgiano‡, C Piffer#, M Bolcato*
and B Brunetti*
*Department of Veterinary Medical Sciences, University of Bologna, 40064
Ozzano dell’Emilia, Italy. Email: [email protected]
†Institute of Veterinary, Animal and Biomedical Sciences, Massey University,
Private Bag 11222, Palmerston North 4442, New Zealand
‡Institute of Genetics, Vetsuisse Faculty, University of Bern, 3001 Bern,
Switzerland.
#Gesundheitbezirk Bozen, Laura Conti Strasse 4, 39100 Bozen, Italy.
During July–November 2013 breeders reported the birth of
Italian Tyrolean Grey calves with abnormally short limbs. Seven calves, aged 2–5 months, were referred to the Department of Veterinary Medical Sciences, Bologna, Italy. Whole genome resequencing of an affected calf detected a single candidate causal mutation in the Ellis van Creveld syndrome 2 (EVC2) gene. All animals had difficulties in assuming or maintaining a quadrupedal stance, with deterioration in this ability with increased growth.
The limbs were disproportionately short and bulky, variably
rotated and arched in a dumbbell-like position. At necropsy, the limbs (in particular the femur and humerus) were rotated and significantly shortened. Histologically the growth plates of long bones and vertebrae were irregular and prematurely closed. The reserve zone showed variable thickness at the expense of proliferative and hypertrophic zones, which were disorganised with multifocal loss
of the normal columnar arrangement. In one calf reduced development of the heart valves was noted. In three female calves the genital tracts appeared fully mature, with numerous follicle-like structures
on the ovaries. The uteri had multiple polypoid structures
La paranoïa et ses environs (sur les traces de D. Cargnello)
No full textSulle tracce del pensiero psicopatologico di D. Cargnello, riteniamo che il concetto di paranoia costituisca, per certe psicosi che si segnalano per la fredda grandiosità e la coerente strutturazione difensiva dell'Io delirante, una categoria nosografica assolutamente non eludibile.
OSSESSIONE E DELIRIO. DUE MOMENTI DI UNA STESSA CRISI DELL'IDENTITA' DELL'IO.
No full textGli autori affrontano il problema della continuità strutturale e tematica fra ossessione e deliri
GLYCOGEN RICH CARCINOMA OF CANINE MALE AND FEMALE MAMMARY GLAND
No full textGLYCOGEN RICH CARCINOMA OF CANINE MALE AND FEMALE MAMMARY GLAND
Muscatello L.V.*[2], Sarli G.[2], Beha G.[2], Benazzi C.[2], Millanta F.[1], Poli A.[1], Asproni P.[1], Giudice C.[3], Levi M.[2],
Brunetti B.[2]
[1]Dip. Scienze Veterinarie ~ Pisa, [2]DIMEVET ~ Bologna, [3]Dip. Scienze Veterinarie Sanità Pubblica ~ Milano
Glycogen rich clear cell carcinoma (GRCC) is a rare subtype of human invasive mammary gland (MG)
carcinoma, in which at least 90% of the neoplastic cells have clear cytoplasm containing glycogen(1). The
aim of this study was to describe the histological, histochemical and immunohistochemical (IHC) features of
GRCC of canine MG.
Serial formalin fixed paraffin embedded tissue sections of two GRCC canine mammary carcinoma, one
female (case I) and one male (case II) were stained with H&E, PAS, PAS diastase (dPAS), and Alcian Blue
(AB). IHC was performed with anti-ER, -PR, -cerbB2, -CK19, -CK14, -CK5/6, -p63, -vimentin, -SMA, -calponin,
-S100, -EGFR, -c-KIT, -E-cad and -ki67 antibodies. Sudan III was carried out on formalin-fixed frozen tissue in
case II.
Histologically, 90% of neoplastic cells showed sharply distinct borders, clear or finely granular cytoplasm
and low N:C ratio. In case II, residual 10% of the neoplastic cells had lipid-like vacuolated cytoplasm. In both
tumors, the cytoplasm resulted strongly positive with PAS. Treatment with diastase abolished PAS
reactivity. Case I showed PAS+ and dPAS- staining also in lymph node metastasis. In case II, 10% of
neoplastic cells were positive to Sudan III. No stain with AB was obtained. Case I showed positivity for CK19
and CK5/6, negativity for ER, PR and c-erbB2, resulting a basal-like phenotype in primary tumor and lymph
node metastasis. They were both positive for EGFR, E-cad, c-KIT, and weakly for calponin. Case II was a
basal-like phenotype, presenting CK 19, E-cad, c-KIT, weak CK14 and strong vimentin positivity. The
proliferative ki67 index was 26.75% in case I and 8.2% in case II.
Based on the morphology, typical features of human MG GRCC are the “fried eggs appearance”, clear
cytoplasm and small dark punctate nuclei (2).On the best of our knowledge, this is the first report regarding
GRCC in canine MG. The diagnosis was confirmed by PAS+ of intracytoplasmic glycogen granules and lack of
stain with dPAS. Case II was considered a GRCC with lipid rich differentiation. Both cases had a basal-like
phenotype and the expression of EGFR and c-KIT was suggestive of an association of cell proliferation with
signal transduction of surface molecules (3). GRCC can be considered a new rare histological subtype of
canine mammary tumors, with clear cytoplasm, PAS+ and dPAS-, expressing the triple negative phenotype,
a tumor with clinical aggressive behavior that should be differentiated from lipid rich carcinoma.
1. Tavassoli, Deville. Lyon: IARC Press, 2003
2. Rosen, Philadelphia. LWW press, 2001
3. Kim et al., Yonsei Med J 53:1142-1146, 2012
ANATOMIA PATOLOGICA
Canine, Mammary carcinoma, Glycogen ric
VARIANTI ANATOMICHE DEL PROCESSO UNICINATO (UP): INCIDENZA NELLE RICOSTRUZIONI CORONALI MPR ALLA TC MULTIDETETTORE
No full textTHE EFFECT OF GLYCEROL ON SHORT-TERM EXPERIMENTAL ENDOLYMPHATIC HYDROPS
No full textThe aim of this paper was to evaluate the effect of one month of treatment with different dosages of glycerol on experimental endolymphatic hydrops produced by obliteration of endolymphatic sac and duct through an extradural approach. Forty-two guinea pigs were used. The animals, divided into six groups, received 0.5-2 gr/kg body weight of the drug per day for one month. The activity of glycerol was determined by statistical analysis of volumetric changes of scala media. Glycerol demonstrated the effect of reducing endolymphatic hydrops. The decrease in hydrops was influenced by the dosage suggesting a stria metabolic response
LA PSICOPATOLOGIA INTERROGA LA FENOMENOLOGIA. ANTICHI PROBLEMI E NUOVE PROSPETTIVE.
No full textGli autori tracciano un profilo del modello antropofenomenologico in psichiatri
An Na-23 and P-31 NMR investigation of sonicated Cardiolipin sodium salt in aqueous medium
No full textThe diagnostic reliability of urinary cytology: A retrospective study.
No full textThe aim of this study was twofold. The first aim was to estimate the diagnostic reliability of urinary cytology for detection and management of urothelial neoplasms by using a specific preserving fluid for sample collection, and the liquid-based thin layer method for specimen preparation, the estimate was based on the correlation between the cytological findings of 10,000 non-hospitalized patients, and their histological diagnoses. A second aim was to compare the reliability of two instruments for thin-layer preparation, i.e., TP2000, TP3000, capable of processing the specimens at very different rates. The preservation of cell structure is ameliorated by the procedure of sample collection and treatment here described. This allows a more accurate reading of LBC slides as shown by: (a) the significant concordance between cytological and histological diagnosis (92%); (b) the significant number of low-grade urothelial carcinomas (20.5%) revealed by urinary cytology and validated by histologic diagnosis; (c) the low rate (8%) of misjudgement of cytological diagnosis reached in this study. The quality of performances of the two instruments tested for thin-layer preparation, i.e., TP2000 and TP3000, is statistically comparable. We recommend the procedure that makes use of preserving fluid for sample collection (cytolytTM) and treatment (preservcyt TM) as here described. We also recommend the use of thin-layer method for specimen preparation since it allows a more uniform distribution of the cells on the support with reduction of overlapping phenomena. Finally, economic considerations suggest the preferential use of Thin Prep 3000. Diagn. Cytopathol. 2011;. © 2011 Wiley-Liss, Inc
- …
