1,721,245 research outputs found
Secondary metabolites "in planta" and "in vivo" cultures of Camptotheca acuminata" Decaisne
No full textOptimization of the production process of dried unripe olives (Olea europaea L.) as a nutraceutical ingredient naturally rich in phenolic compounds
Full text linkPhenols from Olea europaea L. exert several beneficial effects on human health. Olive fruits, particularly the unripe ones, thanks to very high phenols contents (e.g. oleuropein, 80,000 mg/kg), can become a new source of income for olive oil producers, with only a negligible reduction of olive oil production. Aim of this research was to define the best process for obtaining dried unripe green olives very rich in phenols from Olea europaea L. analyzing three typical Tuscan cultivars. Four different freezing methods and different combinations of storage times and temperatures were applied to olives before lyophilization for selecting the best procedure to preserve the native phenols. Moraiolo harvested seven-ten days before complete stone lignification showed to be the most suitable cv for production of the ingredient, with oleuropein 100 g/kg and total phenols 178 g/kg. The application of liquid nitrogen immediately followed by lyophilization gave the best results, while other approaches led to losses of oleuropein of at least 68%. As far as storage before lyophilization, the best performance was for olives frozen in liquid nitrogen kept at −80 °C, with a loss of phenols less than 20% after five months, and oleuropein contents still higher than 80,000 mg/k
New isobaric lignans from refined olive oils as quality markers for virgin olive oils.
Full text linkHerein we describe the influence of olive oil refining processes on the lignan profile. The detection of new
isobaric lignans is suggested to reveal frauds in commercial extra-Virgin Olive Oils. We analyzed five
commercial olive oils by HPLC-DAD-TOF/MS to evaluate their lignan content and detected, for the first
time, some isobaric forms of natural (+)-pinoresinol and (+)-1-acetoxypinoresinol. Then we analyzed
partially and fully-refined oils from Italy, Tunisia and Spain. The isobaric forms occur only during the
bleaching step of the refining process and remain unaltered after the final deodorizing step. Molecular
dynamic simulation helped to identify the most probable chemical structures corresponding to these
new isobars with data in agreement with the chromatographic findings. The total lignan amounts in commercial
olive oils was close to 2 mg/L. Detection of these new lignans can be used as marker of undeclared
refining procedures in commercial extra-virgin and/or Virgin Olive Oils
Ulvan as a source of oligosaccharides for biological applications: enzymatic hydrolysis in a biocompatible medium
Full text linkPhenolic content and in vitro antifungal activity of unripe grape extracts from agro-industrial wastes
No full textThe antifungal activity of unripe grape extracts from agro-industrial wastes has been evaluated against several strains of Candida spp. and dermatophytes. All the extracts tested showed antifungal activity. The geometric mean MIC ranged from 53.58 to 214.31 μg/mL for Candida spp. and from 43.54 to 133.02 μg/mL for dermatophytes. The chemical analyses have been carried out using Liquid Chromatograph equipped with a DAD and MS detectors. Flavan-3-ols were the main metabolites within all samples ranged from 3.3 to 6.8 mg/g fresh weight. For Candida spp. highest negative significant correlation has been found between MICs and polymeric flavan-3-ols (r = −0.842; p < 0.001) and for dermatophytes between MICs and caffeoyl derivatives (r = −0.962; p < 0.01). The results indicate that total extracts obtained from unripe grapes, a large source of waste material derived from the wine industry, could be used as a cheap source of value-added products
In depth study of phenolic profile and PTP-1B inhibitory power of cold-pressed grape seed oils of different varieties
Full text link
GRAPE SEED EXTRACTS AS PROTECTIVE AGENTS AGAINST THE DAMAGES FROM RADIATIONS
No full textThe research developed in this work is part of the PAPARD Project [1] aimed to evaluate the ability of different phenolic extracts to counteract the dangerous effects due to the radiation exposure. The intake of antioxidant extracts can help to protect the astronauts during the space missions but also the common citizens during medical therapies that include exposure to dangerous radiations. The project involved the selection of several herbal extracts or “botanicals” containing different phenols and flavonoids well known for their antioxidant properties and health benefits [2]. Here the results regarding procyanidins in grape seed extracts (GSEs) are reported. The samples were hydroalchoolic extracts of Vitis vinifera cvs Michele Palieri and Italia (cultivated in Italy in 2014 and 2015) and related formulations with inulin or maltodextrins. All the extracts and formulations were irradiated with 140 cSv and their chemical profiles, evaluated in terms of procyanidin content, before and after the radiation treatment, by HPLC-DAD and HPLC-MS-TOF analyses. At the same time, an in vitro evaluation of the antioxidant properties was carried out using the ABTS•+ cation applying pre-selected methods [3,4] after little modifications.
The main findings can be summarized as follow: i) the ratio between the monomeric compounds (catechin and epicatechin) and polymeric flavan-3-ols with (Polimeric Degree ≥ 4) was different in the two cultivar; ii) the treatment with a high dose of radiation induced not significant differences in the composition of the extracts; iii) the antioxidant evaluation confirmed that the radiation treatment does not alter the antioxidants capability of the extract of both Vitis vinifera cultivars.
These findings can be an useful support to design new dietary supplements for helping the reduction of the adverse effects correlated to ionizing radiation treatment in humans
Characterisation of Chlorogenic acid by HPLC-DAD-ESI-MS and HPLC-ESI-MS/MS in Cold Brew and Cold Drip Coffee
No full textA total of 14 different CGAs compounds, in particular five CQAs, one p-CoQA, one FQC, four CQLs, and three diCQAs, were detected. The extraction temperature increase the efficiency of extraction for several constituents from coffee powder more in the cold drip than in the cold brew method
- …
