1,721,019 research outputs found
Bulk RNA sequencing of murine intestinal organoids treated with the factors NRG1, SPP1 or a combination of both
No full textMurine intestinal organoids treated with the factors NRG1, SPP1, a combination of both or with only intestinal medium for 48 hours. Three days after, organoids were collected for RNA extraction and bulk RNA sequencing
Bulk RNA sequencing of sorted intestinal epithelial cells from Wt and Spp1 KO mice upon radiation injury.
No full textMurine intestinal epithelial cells isolated from non-irradiated and irradiated mice Wt or Spp1 KO mice , sorted and processed for RNA extraction following for bulk RNA sequencin
Bulk RNA sequencing of murine intestinal organoids from co-culture of intestinal organoids-macrophages
No full textMurine intestinal organoids co-cultured with pro-inflammatory (M1), anti-inflammatory (M2) and non-polarized (M0) macrophages for 48 hours. On day 3, PI- CD45- EpCAM+ cells from co-culture were sorted for RNA extraction and bulk RNA sequencing
Single cell RNA sequencing of murine intestinal macrophages during homeostasis and radiation
No full textMurine intestinal macrophages (PI- CD45+ F4/80+ CD11b+) isolated from un-irradiated and irradiated (6 days post irradiation) mice, sorted and processed for single cell RNA sequencin
Zebrafish EVL cells in presence or not of bacteria
No full textThe aim was to test the response of early embryos to bacteria. RNA from FACS sorted epithelial cells from early zebrafish embryos was prepared after injecting or not the embryos with E coli. The expression values of all genes analyzed are reported
Single cell RNA sequencing of murine intestinal epithelial organoids treated with Nrg1+Spp1
No full textMurine intestinal epithelial organoids isolated from non-treated and treated condition with Nrg1+Spp1 for four days, and processed for single cell RNA sequencing
Zebrafish EVL cells in presence or not of bacteria 14 hpf
No full textThe aim was to test the response of early embryos to bacteria. RNA from FACS sorted epithelial cells from 14hpf zebrafish embryos was prepared after injecting or not the embryos with E coli. The expression values of all genes analyzed are reported
Single cell RNA sequencing of murine intestinal epithelial (PI- CD45- Epcam+) sorted cells from un-irradiated (control), irradiated (5dpi) and irradiated with macrophages ablation (5dpi + macrophages ablation)
No full textMurine intestinal epithelial cells (PI- CD45- Epcam+) isolated from un-irradiated, irradiated (5 days post irradiation) and irradiated with macrophages ablation (5dpi + macrophages ablation) mice, sorted and processed for single cell RNA sequencin
Single cell RNA sequencing of murine intestinal epithelial sorted cells from non-irradiated (control) mice
No full textMurine intestinal epithelial cells isolated from non-irradiated mice, sorted and processed for single cell RNA sequencing
Comparative genomics of amino acid tandem repeats
No full textTandem amino acid repeats, also known as homopolimeric tract or homopeptides, are very common features of eukaryotic genomes and are present in nearly one-fifth of human encoded proteins. These structures have attracted much interest in the early 1990s when a number of neurological diseases associated with repeat expansion mutations were discovered in humans. Despite their abundance in coding proteins, little is known about their functional consequences. Two scenarios have been proposed. In one, tandem amino acid repeat is considered a neutral structure generated by slippage event and eventually tolerated in protein as long as it does not disrupt the protein function. However, an increasing number of studies proposed that tandem amino acid repeats may be involved in important functional or structural roles. For instance, tandem amino acid repeats had been found to be especially abundant in transcription factors and developmental proteins, where they can potentially modulate protein-protein interaction, exert an effect on gene transcriptional activity, or act as spacer between different protein domains. In addition, several studies have linked changes in repeat size to modification in developmental processes. Despite the advancement made in the last decade, little is known about the selective forces that shape their evolution. The aim of this thesis has been to gain further insight onto the evolutionary dynamics of tandem amino acid repeats by studying the different types of mutations that occur in the amino acid component of the human proteome, by studying the relationship between variability and abundance of amino acid tandem with the evolutionary constraints operating on the proteins, and by studying their conservation and distribution across various vertebrate genomes in both coding and non-coding sequences. The integration of these approaches enabled us to outline an evolutionary model of these structures.Programa de doctorat en Biomedicin
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