7 research outputs found
Characterization of the Xerophyta humilis desiccation induced-1 (Xhdsi-1voc) gene : a member of the Vicinal Oxygen Chelate (VOC) metalloenzyme superfamily upregulated in X. humilis (BAK) DUR and SCHINZ during desiccation
No full textIncludes abstract.Includes bibliographical references (leaves 172-191).Has accompanying material on CD.The resurrection plant, Xerophyta humilis is used as a model system to identify and characterise genes which play an important role in conferring desiccation tolerance in plants. In this study, the expression of a novel gene named desiccation induced-1 (dsi-1VOC) during desiccation in X. humilis and desiccationsensitive plants is characterised
Understanding physiological responses to pre‐treatment inhibitors in ethanologenic fermentations
No full textRetrieval of Full-Length Functional Genes Using Subtractive Hybridization Magnetic Bead Capture
No full textDiverse Exopolysaccharide Producing Bacteria Isolated from Milled Sugarcane: Implications for Cane Spoilage and Sucrose Yield.
No full textBacterial deterioration of sugarcane during harvesting and processing is correlated with significant loss of sucrose yield and the accumulation of bacterial polysaccharides. Dextran, a homoglucan produced by Leuconostoc mesenteroides, has been cited as the primary polysaccharide associated with sugarcane deterioration. A culture-based approach was used to isolate extracellular polysaccharide (EPS) producing bacterial strains from milled sugarcane stalks. Ribosomal RNA sequencing analysis grouped 25 isolates into 4 genera. This study identified 2 bacterial genera not previously associated with EPS production or sucrose degradation. All isolates produced polysaccharide when grown in the presence of sucrose. Monosaccharide analysis of purified polymers by Gas Chromatography revealed 17 EPSs consisting solely of glucose (homoglucans), while the remainder contained traces of mannose or fructose. Dextranase treatment of polysaccharides yielded full digestion profiles for only 11 extracts. Incomplete hydrolysis profiles of the remaining polysaccharides suggest the release of longer oligosaccharides which may interfere with sucrose crystal formation
Population structure of polymer producing bacterial species.
No full text<p>Condensed neighbour-joining phylogenetic tree of isolates identified in milled sugarcane. Homologies of 1321 bp sequences in the 16S rDNA of <i>Leuconostoc</i>, <i>Weissella</i>, <i>Lactobacillus</i> and <i>Salmonella</i> type strains are displayed along with sugarcane associated isolates.</p
EPS production of the various isolates when grown on glucose, fructose or sucrose supplemented media (upper panels)
No full textThe EPS was purified and subjected to digestion by dextranase enzyme, the product of which was separated by Thin Layer Chromatography. These results are summarised in Table 1.</div
Polysaccharide accumulation and susceptibility to dextranase treatment.
No full text<p>A–Sugar dependent or independent EPS production by bacteria grown on glucose, fructose or sucrose. B—Dextranase treatment of EPS. Full digestion is indicted by motile oligosaccharides in comparison to the undigested control; Partial digestion is indicated by an immobile spot at the origin in addition to motile oligosaccharides; Undigested EPS is indicated by no motile oligosaccharides.</p
