17 research outputs found
KAJIAN SOSIOLOGI SASTRA DAN NILAI TOLERANSI PADA NOVEL KAMBING DAN HUJAN KARYA MAHFUD IKHWAN SERTA RELEVANSINYA DENGAN MATERI AJAR APRESIASI SASTRA DI SMA KELAS XII
Get PDFAbstrak: Penelitian ini bertujuan mendeskripsikan: (1) latar sosial novel Kambing dan Hujan, (2) latar belakang sosial pengarang, (3) tanggapan pembaca berdasarkan latar belakang sosialnya, (4) nilai toleransi, (5) relevansi novel Kambing dan Hujandengan materi ajar apresiasi sastra di SMA. Penelitian ini merupakan penelitian kualitatif dengan pendekatan sosiologi sastra.Teknik pengumpulan data yang digunakan adalah analisis dokumen dan wawancara. Analisis data yang digunakan adalah model interaktif.Simpulan penelitian ini adalah:(1) Aspek-aspek latar sosial dalam novel: kebiasaan hidup, adat istiadat, tradisi, cara berpikir dan bersikap, keyakinan, dan pandangan hidup; (2) latar belakang sosial pengarang: lahir di Lamongan, Jawa Timur pada 7 Mei 1980. Tinggal di Yogyakarta dan berprofesi sebagai penulis novel, editor, dan fasilitator di Bengkel Menulis Gerakan Literasi Indonesia. NovelKambing dan Hujan ditulis berdasarkan pengalaman hidupnya saat di Lamongan; (3)tanggapan pembaca, novel tersebut bagus dan menarik serta memberikan pengaruh positif; (4) nilai toleransi dalam novel: nilai toleransi terhadap kebiasaan, keadaan, pandangan, dan kelakuan; (5) novel Kambing dan Hujan relevan dengan materi ajar apresiasi sastra di SMA karena telah memenuhi syarat identifikasi materi ajar pembelajaran dan sesuai dengan materi ajar dalam silabus mata pelajaran bahasa Indonesia Kurikulum 2013 edisi revisi tahun 2016.
Kata kunci: sosiologi sastra, nilai toleransi, materi ajar, novel Kambing dan Hujan.
Abstract: This study aimed to describe: (1) social background novel Kambing dan Hujan, (2) the social background of the author, (3) a response from readers based on social background, (4) the value of tolerance, (5) the relevance of novel Kambing dan Hujan with teaching materials appreciation of literature in high school. This study is a qualitative research approach of sociology of literature. The data collection technique used is the analysis of documents and interviews. Analysis of the data used is an interactive model. The conclusions of this study are: (1) aspects of social background in the novel: habits, customs, traditions, ways of thinking and behaving, beliefs, and outlook on life; (2) social background author: born in Lamongan, East Java, on May 7, 1980. Living in Yogyakarta and work as a novelist, editor, and facilitator in Bengkel Menulis Gerakan Literasi Indonesia. Novel Kambing dan Hujan written based on the experience of author when living in the region of origin; (3) a response from the reader, the novel nice and attractive and provide a positive influence; (4) the value of tolerance in the novel: the value of tolerance to the habit, circumstances, views, and behavior; (5) novel Kambing dan Hujan relevant with teaching materials appreciation of literature in high school because having already qualified identification of teaching material learning and in accordance with the teaching materials in the syllabus Indonesian subjects Curriculum 2013 revised edition 2016.
Keywords: sociology of literature, the values of tolerance, material teaching, novel Kambing dan Hujan
STILISTIKA DAN NILAI PENDIDIKAN KARAKTER NOVEL SEORANG LAKI-LAKI YANG KELUAR DARI RUMAH
Get PDFAbstract:This research aims to describe the use of diction,the figure of speech, the use of image andcharacter education valuesin novel Seorang Laki-Laki Yang Keluar Dari Rumah by Puthut EA. This research employed descriptive qualitative approach with content analysis method. The data of research was contained in novel Seorang Laki-Laki Yang Keluar Dari Rumah and the result of interview with some informants. Technique of collecting data used was library and interview techniques. In this study, the author employed flow model of analysis.The results of research were as follows. Firstly, diction used in novel Seorang Laki-Laki Yang Keluar Dari Rumah consisted of six types. Secondly, there were four figure of speeches used in novel Seorang Laki-Laki Yang Keluar Dari Rumah. Ellipsis as the part of linking figure of speech is used most widely by author. Thirdly, the image used in novel Laki-Laki Yang Keluar Dari Rumah consisted of six types visual images were used most widely. Fourthly, there were ten character education values in novel Seorang Laki-Laki Yang Keluar Dari Rumah. Curiosity education character value was the one used most dominantly. Keywords: diction, figure of speech, image, character education Abstrak:Penelitian ini bertujuan untuk mendeskripsikan penggunaan diksi, gaya bahasa citraan dan nilai-nilai pendidikan karakter dalam novel Seorang Laki-Laki Yang Keluar Dari Rumah karya Puthut EA. Penelitian ini menggunakan pendekatan deskriptif kualitatif dengan metode analisis isi. Data penelitian ini terdapat dalam novel Seorang Laki-Laki Yang Keluar Dari Rumah dan hasil wawancara dengan beberapa informan. Teknik pengambilan sampel yang digunakan dalam penelitian ini adalah purposive sampling.Teknik pengumpulan data dalam penelitian ini menggunakan teknik pustaka dan teknik wawancara dengan model analisis mengalir (flow model of analysis). Berdasarkan hasil penelitian dapat disimpulkan bahwa (1) diksi yang digunakan dalam novel Seorang Laki-Laki Yang Keluar Dari Rumah terdapat enam jenis dan Kata serapan merupakan jenis diksi yang paling banyakdigunakan; (2) gaya bahasa yang digunakan dalam novel Seorang Laki-Laki Yang Keluar Dari Rumah terdapat empat jenis dan elipsis sebagai bagian dari gaya bahasa pertautan paling banyak digunakan; (3) citraan yang digunakan dalam novel Seorang Laki-Laki Yang Keluar Dari Rumah terdapat enam jenis dan citraan penglihatan merupakan jenis citraan yang paling banyak digunakan; (4) nilai pendidikan karakter dalam novel Seorang Laki-Laki Yang Keluar Dari Rumah terdapat sepuluh nilai dan nilai pendidikan karakter rasa ingin tahu yang paling banyak mendominasi. Kata Kunci: diksi, gaya bahasa, citraan dan pendidikan karakter
NOVEL DALAM MIHRAB CINTA KARYA HABIBURRAHMAN EL SHIRAZY (KAJIAN SOSIOLOGI SASTRA DAN NILAI PENDIDIKAN)
Get PDFAbstract: The purposes of research is to describes: (1) element intrinsic which was found in novel Dalam Mihrab Cinta by Habiburrahman El Shirazy; (2) factors behind the creation of novel Dalam Mihrab Cinta by Habiburrahman El Shirazy;(3) education value in novel Dalam Mihrab Cinta by Habiburrahman El Shirazy; and (4) reader conception in novel Dalam Mihrab Cinta by Habiburrahman El Shirazy. This research method was using descriptive qualitative, using the approach of literary sociology. Data sources used were document and informant. The results of this research are: 1) element intrinsic which was found in novel Dalam Mihrab Cinta by Habiburrahman El Shirazy: plot, theme, character, setting, point of view, message; 2) factors behind the creation of novel Dalam Mihrab Cinta by Habiburrahman El Shirazy; author wanted to show the proverbial “becik ketitik ala kethara” that truth will appear and crime will appear later; 3) education value in novel Dalam Mihrab Cinta: religion value, social value, moral value, and esthetic value; 4) In response to readers of the novel Dalam Mihrab Cinta, they generally feel carried away when reading the novel. Keywords: novel, literary sociology, educational value, element intrinsic, response reader Abstrak: Penelitian ini bertujuan untuk mendeskripsikan: (1) unsur intrinsik yang terdapat dalam novel Dalam Mihrab Cinta karya Habiburrahman El Sirazhy, (2) faktor yang melatarbelakangi penciptaan novel Dalam Mihrab Cinta karya Habiburrahman El Sirazhy, (3) nilai pendidikan dalam novel Dalam Mihrab Cinta karya Habiburrahman El Shirazy, (4) tanggapan pembaca mengenai novel Dalam Mihrab Cinta karya Habiburrahman El Shirazy. Penelitian ini berbentuk deskriptif kualitatif, dengan menggunakan pendekatan sosiologi sastra. Sumber data yang digunakan adalah dokumen dan informan. Simpulan penelitian ini adalah: (1) unsur intrinsik yang terdapat dalam novel Dalam Mihrab Cinta karya Habiburrahman El Shirazy: alur, tema, karakter, setting, sudut pandang, pesan, (2) faktor yang melatarbelakangi penciptaan novel Dalam Mihrab Cinta, yaitu adanya “becik ketitik ala kethara” bahwa yang baik akan terlihat dan yang tidak baik akan tampak nantinya, (3) nilai pendidikan dalam novel Dalam, Mihrab Cinta: nilai agama, nilai sosial, nilai moral, dan nilai estetika, dan (4) tanggapan pembaca mengenai novel Dalam Mihrab Cinta, pada umumnya mereka merasa terbawa suasana ketika membaca novel tersebut. Kata kunci: novel, sosiologi sastra, nilai pendidikan, unsur intrinsik, tanggapan pembac
The genome-wide impact of cadmium on microRNA and mRNA expression in contrasting Cd responsive wheat genotypes
Get PDF© 2019 The Author(s). Background: Heavy metal ATPases (HMAs) are responsible for Cd translocation and play a primary role in Cd detoxification in various plant species. However, the characteristics of HMAs and the regulatory mechanisms between HMAs and microRNAs in wheat (Triticum aestivum L) remain unknown. Results: By comparative microRNA and transcriptome analysis, a total three known and 19 novel differentially expressed microRNAs (DEMs) and 1561 differentially expressed genes (DEGs) were found in L17 after Cd treatment. In H17, by contrast, 12 known and 57 novel DEMs, and only 297 Cd-induced DEGs were found. Functional enrichments of DEMs and DEGs indicate how genotype-specific biological processes responded to Cd stress. Processes found to be involved in microRNAs-associated Cd response include: ubiquitin mediated proteolysis, tyrosine metabolism, and carbon fixation pathways and thiamine metabolism. For the mRNA response, categories including terpenoid backbone biosynthesis and phenylalanine metabolism, and photosynthesis - antenna proteins and ABC transporters were enriched. Moreover, we identified 32 TaHMA genes in wheat. Phylogenetic trees, chromosomal locations, conserved motifs and expression levels in different tissues and roots under Cd stress are presented. Finally, we infer a microRNA-TaHMAs expression network, indicating that miRNAs can regulate TaHMAs. Conclusion: Our findings suggest that microRNAs play important role in wheat under Cd stress through regulation of targets such as TaHMA2;1. Identification of these targets will be useful for screening and breeding low-Cd accumulation wheat lines
Optimizing expression and activity of chimeric transposase proteins in human cells
No full text<p><b>Copyright information:</b></p><p>Taken from "Site-directed transposon integration in human cells"</p><p></p><p>Nucleic Acids Research 2007;35(7):e50-e50.</p><p>Published online 7 Mar 2007</p><p>PMCID:PMC1874657.</p><p>© 2007 The Author(s)</p> () Upper panel, schematic overview of transposition assay. Lower panel, fusion protein activity in human cells. Fusion proteins containing variable numbers () of a flexible inter-domain (GGS) peptide linker were tested for transpositional activity in HeLa cells. The (Gly–Gly–Ser) linker supporting the highest level of integration activity was designated L5 for the sake of simplicity. The average number of integration events obtained from three independent transfections is shown (mean ± st.dev.). () Western blot analysis of fusion protein expression. Transfected HeLa cells were harvested two days post-transfection, lysed and subjected to immunoblot analysis using a polyclonal antibody against the transposase. The right panel shows an attempt to normalize HSB5 and fusion protein expression in the cell by transfecting diminishing amounts of the HSB5 plasmid (1×, 0.1× or 0.05×) relative to fusion protein constructs. () Excision activity of chimeric transposases. HeLa cells were transfected with a neomycin-marked (neo) transposon plasmid together with plasmids encoding GFP, HSB5 transposase, or selected fusion proteins. Hirt DNA samples were prepared 2 days later and used as template in a series of nested PCR reactions. Transposon excision and subsequent DNA repair by the host enables the amplification of a diagnostic 253 bp PCR excision-and-repair product. () Sequence analysis of transposon excision and repair products generated from HSB5- and DBD-SB-mediated transposition. Target TA dinucleotides are capitalized. Number of events of each type are shown in parentheses
Fusion protein strategy to achieve site-directed transposon integration
No full text<p><b>Copyright information:</b></p><p>Taken from "Site-directed transposon integration in human cells"</p><p></p><p>Nucleic Acids Research 2007;35(7):e50-e50.</p><p>Published online 7 Mar 2007</p><p>PMCID:PMC1874657.</p><p>© 2007 The Author(s)</p> () Reporter assay for monitoring DNA-binding specificity of candidate DNA-binding domains (DBD). Prospective DBDs were fused to the VP16 activation domain (AD) and expressed from the strong CMV promoter. These activator plasmids were co-transfected into HeLa cells together with a reporter plasmid containing a luciferase gene, a minimal promoter element, and five upstream binding sites for the DBDs (XXXXX) such that co-delivery of an appropriate activator and reporter plasmid results in activation of the downstream luciferase reporter gene. , a synthetic polydactyl zinc-finger protein that recognizes the e2c target site; , the DNA-binding domain from the Gal4 protein that binds to upstream activator sequences (UAS); , the transposase N-terminal 123 amino acid DNA-binding domain that binds to the transposon IDR (inner direct repeat). () DNA-binding specificity of independent protein domains within the context of human cells. Each graph displays luciferase activity relative to transfection with empty vector (pAD). Bars represent the average (mean ± st.dev.) obtained from three independent transfection experiments
