39,023 research outputs found
Infectious structures and response of maize plants to invasion by Exserohilum turcicum in compatible and incompatible host pathogen systems
No full textUse of repetitive extragenic palindromic (REP), enterobacterial repetitive intergenic consensus (ERIC) and BOX sequences to fingerprint Exserohilum turcicum isolates
No full textRace Typing and Evaluation of Aggressiveness of Exserohilum turcicum Isolates of Kenyan, German and Austrian Origin
No full textA review of the Amplified Fragment Length Polymorphism (AFLP) technique in genotyping and DNA fingerprinting studies [Review Article]
No full textABSTRACT Amplified fragment length polymorphism (AFLP) is a marker based on polymerase chain reaction amplification of restricted fragments ligated to synthetic adaptors and amplified using primers which carry selective nucleotides at their 3' ends. The technique generates highly reproducible markers from DNA of any organism and allows high resolution genotyping. AFLP has broad applications and has been used to investigate genomes of different complexity from microbes to higher organisms for purposes of species, strains and varieties identification, systematics, pathotyping, population genetics, simple and complex trait mapping, population genetics, construction of linkage and physical maps. In addition, it is being used in medical diagnostics, forensic analysis and microbial typing. AFLP is superior compared to other markers in that it has time efficiency, generates more information, is highly reproducible and has a wide range of applications. The marker has a drawback in that it generates dominant rather than co-dominant markers and can also be expensive if automated systems are used
Production, purification and separation of phytotoxins from Exserohilum turcicum isolates of Kenyan, German and Austrian origin
No full textRace Typing and Evaluation of Aggressiveness of Exserohilum turcicum Isolates of Kenyan, German and Austrian Origin
No full textEvaluation of Genetic Variability of Kenyan, German and Austrian Isolates of Exserohilum turcicum using Amplified Fragment Length Polymorphism DNA Marker
No full textEighty nine Exserohilum turcicum isolates comprising 56 Kenyan, 26 German and 7 Austrian isolates were isolated from diseased maize plants and cultured on complete liquid medium to generate mycelium for DNA extraction. DNA extraction was done following the CTAB method, DNA purified using spermidin and fingerprinting conducted using Amplified fragment length polymorphism (AFLP) procedure. NTSYSpc, pop gene and Arlequin programs were used to analyze the data and to generate the dendograms. The number of amplified bands and polymorphism varied with the different primer combinations with primer combinations E-ACA/T-CCA, E-ACA/T-CAC, E-ACA/T-CGA, E-ACA/T-CTA revealing a high (79%) level of polymorphism. Cluster analysis of the 607 polymorphic bands from these primer combinations using UPGMA algorithms generated dendograms with 7 main AFLP groups with isolates from different localities grouping together with only two outliers. Pair wise similarity matrix derived with SIMQUAL program showed a wide variation in the AFLP fingerprint of the E. turcicum isolates. Nei’s genetic distance matrix showed that the three populations of E. turcicum isolates differed genotypically with the Kenyan isolates being more genetically related to Austrian isolates (genetic identity of 0.9998) whereas the isolates from Germany and Austria were more diverse (genetic identity of 0.9978). This study showed that AFLP marker is useful in the study of genetic variation of E. turcicum and the pathogen has a high level of genetic diversity
Faust : Romance Of The Secret Tribunals / By George W. M. Reynolds, Author Of "The Mysteries Of London", "Pickwick Abroad", &c. Illustrated In The First Style Of The Art
No full textFAUST : ROMANCE OF THE SECRET TRIBUNALS / BY GEORGE W. M. REYNOLDS, AUTHOR OF "THE MYSTERIES OF LONDON", "PICKWICK ABROAD", &C. ILLUSTRATED IN THE FIRST STYLE OF THE ART
Faust : Romance Of The Secret Tribunals / By George W. M. Reynolds, Author Of "The Mysteries Of London", "Pickwick Abroad", &c. Illustrated In The First Style Of The Art (1)
Cover (1)
Umschlagtitel (8)
Titelseite (9)
Contents (10)
Illustrations / Prologue (11)
Prologue (12)
Chapter I.-XCV. (14)
Epilogue (119
Dr. Duane M. Jackson, Morehouse College, July 2011
No full textThis video is a conversation with Dr. Duane M. Jackson. Dr. Jackson talks about his paper, "Recall and the Serial Position Effect: The Role of Primacy and Recency on Accounting Students' Performance." Jackie Daniel, AUC Woodruff Library, is the interviewer
Perspektywy rozwoju biotechnologii w Polsce
No full textWażnym czynnikiem rozwoju biotechnologii w Polsce są nowoczesne badania naukowe. Artykuł Perspektywy rozwoju biotechnologii w Polsce zwraca uwagę na to, że wiedza z zakresu nauk biologicznych i medycznych przyczynia się do większej konkurencyjności zarówno ośrodków naukowych i badawczych, jak i przedsiębiorstw. Biotechnologiczne projekty naukowo–badawcze realizowane w Polsce nie tworzą jeszcze podstaw do wytwarzania produktów biotechnologicznych w takim zakresie, by polskie przedsiębiorstwa mogły konkurować na rynku międzynarodowych. Jedną z przyczyn, którą analizuje Autor w artykule, jest niewystarczające inwestowanie w rozwój nauki i wsparcie dla małych i średnich firm w sektorze biotechnologii. MSP są najważniejszym elementem rozwoju tego sektora, ponieważ koncentrują się na tworzeniu i rozwoju nowoczesnych produktów biotechnologicznych. Artykuł został podzielony na dwie części: Szanse rozwoju nowoczesnej gospodarki w Polsce bez biotechnologii oraz Edukacja i projekty B+R. Autor finalizuje rozważania, pokazując pozytywny scenariusz dla rozwoju sektora biotechnologicznego w Polsce.Biotechnology uses biological processes in the development of technology or manufacture of a product. It is forecasted that Polish biotechnology industry will exceed very quickly. The technology and science parks in Poland have invested millions Euros to build new laboratories. Polish market is fuelled by increased R&D funding, central and regional governments initiatives. The article on “Polish perspectives of biotechnology development” identifies significant factors for biotechnology project development. It also indicates the examples of government biotechnology initiatives in the world. The author focuses on the statistical analysis of the research and development projects conducted by Polish scientist in scientific laboratories. His main conclusion is that biotechnology will be very important in developing Poland, Polish universities, research and development organizations.Udostępnienie publikacji Wydawnictwa Uniwersytetu Łódzkiego finansowane w ramach projektu „Doskonałość naukowa kluczem do doskonałości kształcenia”. Projekt realizowany jest ze środków Europejskiego Funduszu Społecznego w ramach Programu Operacyjnego Wiedza Edukacja Rozwój; nr umowy: POWER.03.05.00-00-Z092/17-00
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