4 research outputs found
Happiness as a harmonising path for bringing higher education towards sustainability
No full textThis paper is a reflection of an innovative project that complements existing Higher Education for Sustainable Development (HESD) approaches within universities by providing an alternative path to embed sustainable lifestyles within the student population. It presents the SLEUTH project; an initiative born out of the need to reduce energy consumption of students at university but that, due to its systemic design, transforms and goes beyond purely energy reduction and brings Happiness as an approach to build sustainable lifestyles. This is the distinct feature of this initiative, proposing it as adding value to existing HESD approaches. The paper describes and discusses the collaborative building process, implementation and results in detail. The experiences gathered during the project are used and analysed through ‘participant observation’, ‘documents’, ‘questionnaires’, and ‘theme-ing coding’ techniques as a means to understand the richness and diversity of the data resulting from a combination of formal and informal learning settings. Through empirical evidence, this analysis presents ‘real life’ evidence to suggest, firstly the initiative’s design success, its impact on energy consumption and contribution to happiness and sustainable lifestyles. Secondly a successful example for the contribution to existing HESD frameworks that moves away from indoctrination and instead leans towards pro-activeness, self-organisation, and voluntary collective action
sj-docx-1-inq-10.1177_00469580231171014 – Supplemental material for A Cross-Sectional Study on Attitude and Barriers to Interprofessional Collaboration in Hospitals Among Health Care Professionals
No full textSupplemental material, sj-docx-1-inq-10.1177_00469580231171014 for A Cross-Sectional Study on Attitude and Barriers to Interprofessional Collaboration in Hospitals Among Health Care Professionals by Sana Jabbar, Hafiza Shabnum Noor, Ghazal Awais Butt, Syeda Mariyam Zahra, Aleena Irum, Saadia Manzoor, Tahreem Mukhtar and Muhammad Rahil Aslam in INQUIRY: The Journal of Health Care Organization, Provision, and Financing</p
Phospho-dependent modulation of potassium chloride co-transporter KCC2
No full textThe neuronal-specific potassium chloride co-transporter 2, KCC2, is a major
chloride extruder in brain. The expression of KCC2 during neuronal
development is fundamental to the switch of GABAergic response from
excitatory to inhibitory. Malfunction of KCC2 can cause impairment of
chloride homeostasis in neurons and is implicated in neurological disorders
such as epilepsy.
To date the role of protein phosphorylation in the regulation of KCC2 remains
elusive. In this thesis, direct phosphorylation of KCC2 by PKC and Src
tyrosine kinase was shown in vitro and in cultured neurons using the
radioactive isotope 32P. Single mutation of serine residue at position 940 in the
intracellular domain of KCC2 (Ser940) to alanine (S940A) blocked the
phosphorylation of KCC2 under PKC activation. However, tyrosine
phosphorylation of KCC2 was shown to not affect Tyr1087, the putative tyrosine
kinase phosphorylation site. To better understand phosphorylation of KCC2 at
Ser940, a phospho-specific antibody against this residue - namely p-S940 - was
developed. Interestingly, agents inhibiting PKC and phosphatases altered signal
of p-S940, indicating involvement of PKC, phosphatase-1 (PP1) and
phosphatase-2A (PP2A) in the regulation of Ser940 phosphorylation. In an in
vitro method using p-S940, it was shown that PP1 and PP2A dephosphorylated
KCC2
