8 research outputs found
Study on the Beneficial Effects of Garlic, Tribulus Terrestris, and Vitamin E Supplementation on Semen Quality, Fertility, and Hatchability of Desi Male Chicken
This study evaluated the effects of dietary supplementation with garlic, Tribulus terrestris, and vitamin E on semen quality parameters in desi male chickens maintained under local farming conditions in Sindh, Pakistan. A total of twenty-four non-descriptive desi chickens (8 males and 16 females, aged 19–20 weeks) were allocated to four dietary groups for 42 days: G1 (basal diet with 150 g/kg garlic), G2 (basal diet with Tribulus terrestris at 1 capsule/liter of water), G3 (basal diet with vitamin E at 1 capsule/liter of water), and G4 (control with basal breeding ration only). Semen samples collected from treated males were analyzed for volume, mass motility, individual motility, live sperm, and dead sperm counts. Results demonstrated significant improvements in the garlic group (G1), with semen volume (0.36 ± 0.02 ml), mass motility score (4.40 ± 0.16), and individual motility (83.00 ± 2.55), outperforming the control group (0.21 ± 0.02 ml, 2.60 ± 0.16, and 62.60 ± 1.67 respectively). Tribulus terrestris (G2) and vitamin E (G3) groups also showed improved semen characteristics relative to control, though garlic supplementation consistently yielded the highest values. These findings indicate that targeted inclusion of garlic, Tribulus terrestris, or vitamin E in breeding rations can significantly enhance semen quality traits in desi chickens, offering practical, low-cost strategies for improving reproductive efficiency and supporting sustainable poultry production in resource-limited settings
Impact of Chilling, Freezing, and Repeated Freeze–Thaw Cycles on Physical Quality of Buffen, Chevon, and Chicken Meat
Chilling, freezing, and repeated freeze–thaw cycles are common meat preservation methods; however, they can alter key physicochemical properties. Comparative data across different species under standardized conditions are limited. To compare the effects of chilled storage, frozen storage, and repeated freeze–thaw cycles on pH, water-holding capacity (WHC), thaw loss, cooking loss, and drip loss in buffen (buffalo), chevon (goat), and chicken meat. Longissimus dorsi muscles from buffalo and goat, and breast meat from broiler chickens, were stored under chilled (2–4 °C), frozen (–10 to –12 °C), or repeated freeze–thaw conditions. Measurements were performed at defined intervals of chilling, freezing and freeze thaw cycles, and data were analyzed by one-way ANOVA with Tukey’s HSD (P ≤ 0.05). The pH declined significantly (P ≤ 0.05) in all treatments, with the steepest reductions in repeatedly thawed samples. WHC decreased from 63.32 %, 69.98 %, and 68.00 % in fresh buffen, chevon, and chicken, respectively, to 45.94 %, 48.61 %, and 47.64 % after 15 days of chilled storage. Thaw loss and cooking loss increased markedly over time, especially in freeze–thaw treatments, reaching 26.35 %, 25.77 %, and 26.15 % for thaw loss and 64.66 %, 61.19 %, and 65.44 % for cooking loss at the final intervals. Drip loss rose progressively, with chilled and repeatedly thawed samples showing the highest increases. Extended storage and repeated freeze–thaw cycles significantly reduce meat quality across all species, with repeated thawing causing the most pronounced deterioration. Minimizing freeze–thaw cycles are essential for preserving physicochemical quality during meat handling, distribution, and retail
Fixed-time artificial insemination technology in buffaloes: a review
Buffalo occupies a leading position as a major livestock commodity and is the primary milk-producing animal in many countries like Italy, China, India, Pakistan, Bangladesh, and Nepal. Buffalo farming emphasizes the significance of effective reproductive strategies. Among effective reproductive strategy, artificial insemination has a significant influence on herd's genetic progress. Nonetheless, buffaloes exhibit unique reproductive behavior, which complicates the insemination process. These animals demonstrate inconsistent periods (ranging from 6–48 h) of mounting acceptance. Therefore, timed artificial insemination (TAI) has surfaced as a useful technique for advancing buffalo breeding initiatives and omits the need for heat detection. TAI enhances reproductive management and genetic progress in buffaloes by synchronizing estrus and optimizing insemination timing. This review focuses on examining buffalo reproductive physiology, particularly emphasizing estrus synchronization protocols, ovulation, and TAI. We also provide a brief description of the factors influencing TAI success, such as hormonal treatments and environmental conditions. This review underscores TAI's importance identifies areas for further research and development and reinforces its central role in sustainable buffalo farming
Trypanosomiasis and its Diagnostic Techniques in Camel: a Comprehensive Review
Trypanosomiasis, a parasitic disease caused by protozoan parasites of the genus Trypanosoma, poses a significant threat to camel populations worldwide. This study designed for the diagnostic techniques used for detecting Trypanosoma infections specifically in camels. The disease, commonly known as camel trypanosomiasis or surra, adversely impacts the health and productivity of camels, thereby affecting livelihoods dependent on these animals. Various diagnostic methods are employed for the detection of Trypanosoma infection in camels, including microscopic examination of blood smears, serological tests like ELISA (Enzyme-Linked Immunosorbent Assay), and molecular techniques such as PCR (Polymerase Chain Reaction). Each method has its strengths and limitations in terms of sensitivity, specificity, and practicality in field settings. Efforts to control and manage camel trypanosomiasis rely heavily on accurate and timely diagnosis. Early detection facilitates prompt treatment and prevents the spread of the disease within camel herds. Additionally, the development and optimization of diagnostic tools tailored specifically for camels are crucial for effective disease surveillance and control. This study underscores the importance of ongoing research and innovation in diagnostic methodologies to combat trypanosomiasis in camel populations efficiently
Prevalence of Abortifacient Disorders in Small Ruminants: a Comprehensive Review
Abortifacient disorders in small ruminants present a serious problem for livestock farmers, leading to financial losses and lower production rates. This review examines both infectious and non-infectious factors that cause abortions in goats (Capra hircus) and sheep (Ovis aries), which are crucial animals in rural farming practices. Infectious causes, such as bacteria (Brucella melitensis, Chlamydia abortus, Coxiella burnetii), viruses (bluetongue, border disease), and parasites (Toxoplasma gondii), are major factors in reproductive health problems, resulting in considerable livestock losses. Additionally, non-infectious factors like poor nutrition, toxins, and genetic issues also contribute to these reproductive challenges. Diagnosing the causes of abortion often requires complex testing of tissue samples, emphasizing the importance of advanced diagnostic tools. Preventing these disorders through vaccinations, better management practices, and health programs is key to minimizing their impact. This is particularly important in countries such as Pakistan, where small ruminants are essential to sustaining the agricultural economy
Brucellosis and its Diagnostic Techniques in Animals: a Comprehensive Review
Brucellosis is a highly zoonotic infection found in animals and human, it has a high death ratio and lifetime sterility rate. The rates of intra- and interspecific infections have dramatically increased in recent years as a result of inadequate management and scarce resources particularly in developing nations. In cattle, reduced milk yield and elevated body temperature are the primary indicators, whereas in humans, soaring fever, overall malaise, and arthritis are the hallmarks of late-term abortion. Despite the fact that this diseases in animals and humans found unclear clinically, it will leads towards the hindrance in diagnosing this infectious illness serologically, PCR is an only way to diagnose this illness accurately. In developing nations, brucellosis poses a significant risk to livestock and public health because of its intricate nature. With an emphasis on developing nations specifically, we outlined the most recent research, noteworthy developments, and difficulties in the management and immunization against brucellosis in this review
Study on Predisposing Factors and Control Measures against Cryptosporidiosis in Buffalo Calves at Cattle Colony Hyderabad, Sindh, Pakistan
The current study looked at the variables affecting the frequency of Cryptosporidium Parvum in buffalo calves at the Hyderabad Cattle Colony. From calves, 100 fecal samples were taken in total, preserved, and analyzed using direct smear and Ziehl-Neelsen staining methods. 74 percent of the samples tested positive for Cryptosporidium Parvum. Disease rates were higher in Nili Ravi calves (86 %) compared to Kundhi calves (62 %), with a statistically significant difference (
A Comprehensive Study on One Health Strategy and Public Health Effects of Salmonella
Salmonella poses a significant public health challenge due to its antibiotic resistance, zoonotic transmission, and diverse clinical manifestations. Over 60 % of human diseases are zoonotic, influenced by ecological dynamics and human activities such as land use changes, population growth, and international travel. Foodborne diseases, particularly those caused by Salmonella spp., have a substantial global impact, especially in low-income countries. Salmonella is divided into two species, Salmonella enterica and Salmonella bongori, with more than 2000 serotypes and six subspecies within S. enterica. This bacterium is highly adaptable, surviving extreme conditions such as drying, high salt concentrations, and acidic environments. Detection methods typically involve pre-enrichment, followed by specific enrichment, plating, and identification through serological and molecular techniques like PCR. Severe cases, especially in vulnerable populations, often require antimicrobial treatment. Salmonella’s pathogenicity is driven by its ability to invade, persist, and replicate within host cells, facilitated by Salmonella pathogenicity islands (SPIs) and type III secretion systems. Epidemiological data reveal a global distribution of enteric fever, with high incidence and mortality rates in Africa, Asia, and South America, while lower rates are reported in developed regions, often linked to international travel. This study adopts a One Health approach, examining Salmonella\u27s resistance, zoonotic transmission, and public health impact, while suggesting innovative strategies for detection and control to mitigate its global effects
