5 research outputs found
Extraction and Estimation of Protein from Ginger (Zingiber officinale) and its Interaction with Glucose Molecule
No full textAim: To evaluate the protein composition in the ginger rhizome and its interaction with glucose molecule.
Place and Duration of Study: Department of Biochemistry, Sokoto State University, Sokoto, Nigeria, between April 2021 and May 2021.
Methodology: Protein was extracted from the rhizome of ginger using 0.1 M phosphate buffer. The protein concentration of the sample was estimated using Biuret method while using xanthoproteic test, the presence of aromatic amino acids was ascertained. The crude protein sample was interacted with glucose using UV interaction study.
Results: The protein concentration of the sample (2 g) was found to be 1.702 mg/ml, it was identified that ginger rhizome contains aromatic amino acids. UV interaction study between the crude protein sample and glucose molecule showed an increase in absorbance at the range of 280 nm.
Conclusion: The interaction of ginger with glucose shows that it possesses a hypoglycemic effect
Comparison of Antidiabetic Effect of Ethanolic Leaves Extract of Mangifera indica and Moringa oleifera on Alloxan Induced Diabetic Rats
No full textBackground: Diabetes mellitus (DM) is one of the leading metabolic disorder as well as among the major cause of death in developing countries. Several plants were investigated as a possible remedy for the management of DM, however, Moringa oleifera (MO) is one of the widely used plants. Thus, the high demand and scarcity of MO in certain places necessitate an alternative plant for management of DM.
Aim: The aim of this study is to compare the antidiabetic effects of Mangifera indica (MI), MO and combinatorial formulation of ethanolic extract of both plants (MOMI).
Methods: Diabetes was induced by intraperitoneal injection of 100 mg alloxan per kg body weight. Diabetes was confirmed in experimental animals three days after the injection. MI, MO and MOMI (a mixture of both) were administered to groups of animals receiving MI, MO and MOMI respectively. Blood glucose level was estimated three weeks after treatment and one week after withdrawal of treatment.
Results: The blood glucose of animals of all groups reduced significantly (P < 0.01) compared to diabetic control (DC) group. A significant increase in blood glucose (P < 0.01) in animals of MI group was observed one week after withdrawal of treatment whereas, the increase in MO and MOMI groups were statistically insignificant. Furthermore, a significant increase in body weight (P < 0.01 and P < 0.05) was observed in treated groups (especially MOMI) compared to DC group.
Conclusion: The results of the study showed MO has a more antidiabetic effect compared to MI. Combination of both at 1:1 increases the antidiabetic effect of MI. Increase in body weight could not be a direct influence of the leaves. Hence mixing MO and MI may be a good alternative for managing DM.
 
Impact Survey Results after SAFE Strategy Implementation in 15 Local Government Areas of Kebbi, Sokoto and Zamfara States, Nigeria.
Get PDFPURPOSE: To determine prevalence of trachoma after interventions in 15 local government areas (LGAs) of Kebbi, Sokoto and Zamfara States, Nigeria. METHODS: A population-based impact survey was conducted in each LGA using Global Trachoma Mapping Project (GTMP) protocols. In each LGA, 25 villages were selected, except in Arewa LGA, where we selected 25 villages from each of four subunits to obtain finer-resolution prevalence information. Villages were selected with probability proportional to size. In each village, 25 households were enrolled and all consenting residents aged ≥1 year were examined by GTMP-certified graders for trachomatous inflammation-follicular (TF) and trachomatous trichiasis (TT). Information on sources of household water and types of sanitation facilities used was collected through questioning and direct observation. RESULTS: The number of households enrolled per LGA ranged from 623 (Kware and Tangaza) to 2488 (Arewa). There have been marked reductions in the prevalence of TF and TT since baseline surveys were conducted in all 15 LGAs. Eight of the 15 LGAs have attained TF prevalences <5% in children, while 10 LGAs have attained TT prevalences <0.2% in persons aged ≥15 years. Between 49% and 96% of households had access to water for hygiene purposes within 1 km of the household, while only 10-59% had access to improved sanitation facilities. CONCLUSION: Progress towards elimination of trachoma has been made in these 15 LGAs. Collaboration with water and sanitation agencies and community-based trichiasis surgery are still needed in order to eliminate trachoma by the year 2020
