112 research outputs found

    Selection of frost-tolerant cell lines from cell cultures of Solanum tuberosum L.

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    Fourteen hydroxyproline-resistant cell lines were selected by plating 7 days old cell suspensions of Solanum tuberosum L. cvs. Desiree and Maris Piper on a cell plating medium containing 5 or 10 mM hydroxyproline (hyp). Cell suspensions were either plated directly on selective media or after mutagenic treatment with gamma rays at a dose of 20 Gy or after freezing to –6°C. The frequency of resistant colonies varied from 0.15 to 0.35 x 10-6. Almost all the selected lines possessed increased levels of frost tolerance as compared to their non-selected controls except one indicating that hyp resistance and frost tolerance are not necessarily linked

    Selection of frost-tolerant cell lines from cell cultures of Solanum tuberosum L.

    No full text
    Fourteen hydroxyproline-resistant cell lines were selected by plating 7 days old cell suspensions of Solanum tuberosum L. cvs. Desiree and Maris Piper on a cell plating medium containing 5 or 10 mM hydroxyproline (hyp). Cell suspensions were either plated directly on selective media or after mutagenic treatment with gamma rays at a dose of 20 Gy or after freezing to –6°C. The frequency of resistant colonies varied from 0.15 to 0.35 x 10-6. Almost all the selected lines possessed increased levels of frost tolerance as compared to their non-selected controls except one indicating that hyp resistance and frost tolerance are not necessarily linked

    Selection of frost-tolerant cell lines from cell cultures of Solanum tuberosum L.

    No full text
    Fourteen hydroxyproline-resistant cell lines were selected by plating 7 days old cell suspensions of Solanum tuberosum L. cvs. Desiree and Maris Piper on a cell plating medium containing 5 or 10 mM hydroxyproline (hyp). Cell suspensions were either plated directly on selective media or after mutagenic treatment with gamma rays at a dose of 20 Gy or after freezing to –6°C. The frequency of resistant colonies varied from 0.15 to 0.35 x 10-6. Almost all the selected lines possessed increased levels of frost tolerance as compared to their non-selected controls except one indicating that hyp resistance and frost tolerance are not necessarily linked

    EFFECT OF VARIOUS CALCIUM SALTS ON RIPENING OF MANGO FRUITS

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    Abstract: Green mature fruits of mango cv. SS-1 (Kala Chaunsa) were immersed for 10 minutes in 2.5, 5.0 or 7.5% calcium chloride (CaCl 2 .2H 2 O), calcium sulphate (CaSO 4 .2H 2 O) or calcium ammonium nitrate {Ca(NH 4 NO 3 ) 2 } solutions. A control was also included in which fruits were dipped in fresh water for 10 minutes. The fruits were ripened at ambient temperature (25 ± 3 o C) in boxes lined and covered with newspaper. Calcium chloride delayed the fruit ripening about 3 days as compared to control and resulted in better aroma of the fruits, however, it induced skin shrivelling. Calcium sulphate treatments resulted in improved pulp colour. The increase in concentration of calcium salts resulted in delayed ripening but had negative effect on fruit quality by increasing skin shrivelling and lowering flavour and taste of the fruits. Calcium chloride at 5.0% delayed the ripening for 4 days and resulted in better skin and pulp colour but with increased skin shrivelling and poor flavour and taste, indicating poor eating quality

    USE OF CONDUCTIVITY MEASUREMENTS FOR THE DETERMINATION OF GROWTH RATE OF SOLANUM TUBEROSUM L. CELL CULTURES

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    The suitability of medium conductivity measurements for determining growth in plant cell culture was tested using the suspension cultures of potato (Solanum tuberosum L.) cvs. Desiree and Maris Piper. A perfect positive relationship between packed cell volume and cell number was found during the growth of cells in suspension culture. A perfect negative correlation between increase in packed cell volume and decrease in medium conductivity was observed from the experiment. The measurement of cell concentration by cell counting also showed a negative correlation with the conductivity of culture medium in both cultivars tested. Regression analysis was used to know the rate change in the dependent variable subject to a unit change in explanatory variabl

    Assessment of Different Conventional and Biofortified Wheat Genotypes Based on Biology and Damage Pattern of Rhyzopertha dominica and Trogoderma granarium

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    The lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and khapra beetle, Trogoderma granarium E. (Coleoptera: Dermestidae) are primary stored-grain insect pests. Differences in certain biological and physical parameters of both pest species and wheat genotypes were investigated under laboratory conditions. Zinc (Zn)-biofortified (Zincol-2016 and Akbar-2019) and conventional (Arooj-2022, Nawab-2021, Dilkash-2021, Bhakkar Star-2019) wheat genotypes were used in this study. Zn-biofortified genotypes outperformed the conventional ones, with significant differences observed in fecundity, percent adult emergence, total developmental duration, percent grain damage, and weight loss of both insect species. The results further revealed that the fecundity of R. dominica and T. granarium were lowest on Akbar-2019 in both the free-choice test (42.50 and 33.17) and no-choice test (35.50 and 32.50), respectively. Similarly, percent adult emergence of both insect species was also lowest on Akbar-2019 in both the free-choice test (69.78 and 70.28%) and no-choice test (67.38 and 70.71%). The total developmental period also showed significant variation among the tested genotypes. The longest developmental period was recorded in Akbar-2019, i.e., 44.33 and 58.83 days, for R. dominica and T. granarium, respectively. Similarly, percent grain damage (13.23 and 10.33%) and weight loss (3.62 and 2.12%) were found to be minimum in Akbar-2019 for both pest species, respectively. Additionally, a positive correlation was observed between grain moisture content and damage parameters, suggesting that the higher moisture content may aggravate the percent grain damage and weight loss. These findings indicate that the nutritional qualities of Zn-biofortified wheat genotypes negatively affected the development of both insect species; thus, it can be an efficacious approach not only for ensuring food security but also for protecting grains against storage pests
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