3,703 research outputs found
Solid-State NMR Determination of Protein Secondary Structure
No full textThis work focused on developing solid-state NMR pulse sequence to determine the protein secondary structure. Chapter 1 gives a basic view of using solid-state NMR to obtain the structure information. The main thesis was discussed in four parts: Ch.2: R-TOBSY: isotropic polarization transfer in biological solids. Ch.3: MCSP: Multiple Chemical Shift Precession for selective polarization transfer. Ch.4: psi angle determination: using correlation between C' CSA and Ca−Ha tensors.
Ch.5: CONGEE: Correlation Of Non-Gamma Encoded Experiment.
Each part is self-contained. The successful experiments of R-TOBSY (Chapter 2) and MCSP (Chapter3) in biological solids provide a crucial step for the psi angle determination in Chapter 4 and Chapter 5. Chapter 4~5 discussed the spin physics of the RACO experiment originally introduced by Ishii and co-workers in 1996. Through the analytical analysis, the optimal RACO experiment was designed and was employed for determination of the dihedral angles of L-alanine and DL-NAV. Brief conclusion and outlook were summarized in chapter 6.Chapter 1 Structure Determination by Solid-State NMR 1
1.1 Solid-State NMR versus Solution-State NMR 1
1.2 Pulse Sequence in Static Solid-State NMR 2
1.3 Magic Angle Spinning (MAS) 3
1.4 Pulse Sequence under MAS 4
1.5 Structure Information Determined by SSNMR 4
1.5.1 Chemical-shift based conformation determination 4
1.5.2 Distance constraints 5
1.5.3 Angular constraints 5
1.6 Summary 9
Chapter 2 R-TOBSY without proton decoupling 13
2.1 Introduction 13
2.2 Brief Review of TOBSY-Like Sequences 14
2.3 Simulations on No Decoupling R-TOBSY 16
2.4 Experimental 19
2.4.1 Sample preparation 19
2.4.2 Solid-state NMR 19
2.5 Results and Discussion 20
2.6 Conclusion 24
Chapter 3 Selective Polarization Transfer Based on
Multiple Chemical Shift Precession 28
3.1 Introduction 28
3.2 Theory 29
3.2.1 Two-spin system 29
3.2.2 Three-spin system 32
3.3 Experimental 32
3.4 Results and Discussion 34
Chapter 4 Psi Angle Determination by RACO 42
4.1 Introduction 42
4.2 Theory 43
4.2.1 Correlation analysis 44
4.2.2 Numerical simulations 46
4.2.3 General consideration 47
4.2.4 Sensitivity to Psi angle 52
4.2.5 Experimental consideration 53
4.3 Experimental 54
4.3.1 Sample preparation 54
4.3.2 NMR experiments 54
4.3.3 Numerical simulations 55
4.3.4 Data process 56
4.4 Results 56
4.4.1 1D spectra 56
4.4.2 2D spectra 56
Chapter 5 Correlations of Non-Gamma Encoded Experiments 62
5.1 Introduction 62
5.2 Correlation Analysis 63
5.3 Rotor-phase Encoded Experiments 68
5.4 Magnetization-Phase Encoded Experiments 76
5.5 Discussion of Rotor-Phase Encoded and Magnetization
Encoded Experiments 79
5.6 Supercycle-Phase Encoded Experiments 82
Chapter 6 Conclusions and Outlook 8
A letter from Mou Zuoyun to Dr. Edward Steitz (January 20, 1979)
No full textThis is a copy of a letter sent from Mou Zuoyun (牟作云), Springfield College grad of 1947 and, at the time, head of the All-China Federation and the Chinese National Basketball teams, dated January 20, 1979. In the letter he talks a bit about his father-in-law, John Ma (Ma Yuehan) and apologizes for not being about to arrange a visit to China for the Springfield College Men's Basketball team for this year.Mou Zouyun (牟作云) was born in 1913 in Wuqing district, Tianjin. In 1934, while he was still in high school, Mou was selected to Chinese National Basketball team for the 10st Far East Asian Olympic Games. Mou had a nickname “Mo Zhe Yun”, which means, “touch the clouds”. Mou also participated in track and field in Far East Asian Games in 1934. Later in 1934, He was admitted to Beiping Normal University under Shouyi Dong and became one of the “Five Tigers”. The “Five Tigers” won the National Championships in China, and the players, including Mou, became members of the Chinese National Basketball Team in 1936 Berlin Olympic games.
Mou married John Ma’s second daughter, Ma Peilun, in April 1944. He then came to Springfield College for a two-year graduate program along with Ma Qiwei, John Ma’s son, in June 1946. While here, he was known as James (Jimmy) T. Y. Mao. Mr. Mou received a Bachelor degree from Springfield College in 1947. Mr. Mou expected to continue his graduate program, when he was appointed as the basketball coach of Chinese Olympic team for the 1948 Olympic, so he had to cut off his graduate study and return to China to take up his role in June 1947.
Mou is regarded as a pioneer of professional basketball of the People’s Republic of China. In recognition for his achievement, Mou was conferred the title of Lifetime Honorary President of the Asian Basketball Association and was made appointed a lifetime Honorary Committee position on FIBA. The championship title of the Chinese Basketball Association League was named as “Mou Zuoyun Cup” in 2005. And he was praised by Yao Ming in his induction speech to the Basketball Hall of Fame ceremony in 2016.
Mou passed away in 2007.
For biographical information on Edward S. Steitz, see: https://springfield.as.atlas-sys.com/agents/people/110
Corrigendum to “Double-quantum NMR spectroscopy based on finite pulse RFDR”[Solid State NMR 27 (2005) 266–270]
No full textEfficient spin–spin scalar coupling mediated C-13 homonuclear polarization transfer in biological solids without proton decoupling
No full textDetermination of the backbone torsion psi angle by tensor correlation of chemical shift anisotropy and heteronuclear dipole–dipole interaction
No full textA memo and attachments to Vernon Cox from Edward Steitz about Mou Zuoyun (Nov. 20, 1978)
No full textThis is a copy of a memorandum to Springfield College professor Vernon Cox from Dr. Edward Steitz, Athletic Director of Springfield College, about Mou Zouyun (牟作云), known to Springfield College as Mu Tsu-Yun in the documentation here. The memo has a few notes made by Vernon Cox on Mou and other Springfield alum in China. The attachments are copies of an All-China Sports Federation that has a photo of Mou and information on the the delegation that came over from China in 1978.Mou Zouyun (牟作云) was born in 1913 in Wuqing district, Tianjin. In 1934, while he was still in high school, Mou was selected to Chinese National Basketball team for the 10st Far East Asian Olympic Games. Mou had a nickname “Mo Zhe Yun”, which means, “touch the clouds”. Mou also participated in track and field in Far East Asian Games in 1934. Later in 1934, He was admitted to Beiping Normal University under Shouyi Dong and became one of the “Five Tigers”. The “Five Tigers” won the National Championships in China, and the players, including Mou, became members of the Chinese National Basketball Team in 1936 Berlin Olympic games.
Mou married John Ma’s second daughter, Ma Peilun, in April 1944. He then came to Springfield College for a two-year graduate program along with Ma Qiwei, John Ma’s son, in June 1946. While here, he was known as James (Jimmy) T. Y. Mao. Mr. Mou received a Bachelor degree from Springfield College in 1947. Mr. Mou expected to continue his graduate program, when he was appointed as the basketball coach of Chinese Olympic team for the 1948 Olympic, so he had to cut off his graduate study and return to China to take up his role in June 1947.
Mou is regarded as a pioneer of professional basketball of the People’s Republic of China. In recognition for his achievement, Mou was conferred the title of Lifetime Honorary President of the Asian Basketball Association and was made appointed a lifetime Honorary Committee position on FIBA. The championship title of the Chinese Basketball Association League was named as “Mou Zuoyun Cup” in 2005. And he was praised by Yao Ming in his induction speech to the Basketball Hall of Fame ceremony in 2016.
Mou passed away in 2007.
For biographical information on Edward S. Steitz, see: https://springfield.as.atlas-sys.com/agents/people/110
Jia ru ju he wu dui jun yun tuan liu ji jun yun tuan liu dui liu de ying xiang
No full textWong, Chai Kwok = 加入聚合物對均勻湍流及均勻湍流對流的影響 / 黃濟國.Thesis M.Phil. Chinese University of Hong Kong 2013.Includes bibliographical references (leaves 89-91).Abstracts also in Chinese.Title from PDF title page (viewed on 01, November, 2016).Wong, Chai Kwok = Jia ru ju he wu dui jun yun tuan liu ji jun yun tuan liu dui liu de ying xiang / Huang Jiguo
Heyuanninae Yun, 2019, clade. nov
No full textBarsbold (1981) erected Ingenia yanshini Barsbold, 1981 for a nearly complete oviraptorid skeleton from the Upper Cretaceous (Campanian-Maastrichtian) Barun Goyot Formation in Ömnögov, southern Mongolia. Moreover, he erected a subfamily-level clade Ingeniinae Barsbold, 1981 to distinguish Ingenia yanshini from other oviraptorids such as Oviraptor philoceratops Osborn, 1924 (Barsbold, 1981). When Barsbold first erected the subfamily, he considered this subfamily as monotypic (Barsbold, 1981) but now this subfamily comprisesmultiple taxa such as Machairasaurus leptonychus Longrich, Currie, and Dong, 2010 and Heyuannia huangi Lü, 2002. However, the genus name Ingenia is a junior homonym of the tripyloidid nematode genus Ingenia Gerlach, 1957 and according to the Article 23.1 of the International Code of Zoological Nomenclature (ICZN, 1999), Gerlach’s 1957 name has priority. Therefore, the usage of the subfamily name Ingeniinae is problematic as it is based on a junior homonym. Paul (1988) suggested a new combination Oviraptor yanshini for “ Ingenia ” yanshini but this classification has not been supported by any subsequent authors (Easter, 2013). Easter (2013) proposed a new genus name Ajancingenia Easter, 2013 for “ Ingenia ” yanshini, but subsequent phylogenetic analyses have found “ Ingenia ” yanshini and Heyuannia huangi as sister taxa (e.g., Lamanna et al., 2014; Funston and Currie, 2016; Lü et al., 2016; Funston et al., 2018). Therefore, most recent studies have considered Ajancingenia as junior synonym of Heyuannia and used new combination Heyuannia yanshini for “ Ingenia ” yanshini (e.g., Funston et al., 2018; Herne et al., 2018). Thus, Heyuanniais the proper synonym available as a replacement name of the junior homonym. Therefore, the author proposes a new replacement subfamily name Heyuanninae clade. nov. (from Heyuannia and –inae) for “Ingeniinae”. Osmolska et al. (2004) defined “Ingeniinae” as Conchoraptor gracilis, Ingenia yanshini, their most recent common ancestor, and all descendants. Here the author revises this definition for Heyuanninae, using Heyuannia huangi as the specifier. Heyuanninae is a node-based subfamily-level clade that can be defined as the least inclusive clade containing Heyuannia huangi and Conchoraptor gracilis.Published as part of Yun, Chan-Gyu, 2019, Heyuanninae clade nov., a replacement name for the oviraptorid subfamily " Ingeniinae " Barsbold, 1981, pp. 295-296 in Zootaxa 4671 (2) on page 295, DOI: 10.11646/zootaxa.4671.2.11, http://zenodo.org/record/344216
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