1,721,020 research outputs found
Inmunohistochemistry in bovine fetal tissues for the diagnosis of campylobacter fetus spp
No full textTesis presentada para optar al título de Especialista en Salud Animal, de la Universidad Nacional de Mar del Plata, en diciembre de 2021La campylobacteriosis bovina es una enfermedad infecciosa que produce infertilidad y abortos en los rumiantes. Las principales especies implicadas son Campylobacter fetus spp. venerealis (CFV), Campylobacter fetus spp. fetus (CFF) y Campylobacter jejuni. El diagnóstico del aborto por campylobacteriosis en los fetos se realiza mediante el aislamiento de la bacteria en cultivos especiales y por inmunofluorescencia directa (IFD) a partir del contenido de abomaso y pulmón. La inmunohistoquímica (IHQ) es otra herramienta diagnóstica que permite realizar estudios retrospectivos y asociar de manera precisa el antígeno con las lesiones. Asimismo, esta técnica es relevante cuando los fetos se encuentran depredados, autolíticos y/o contaminados, ya que el cultivo bacteriológico es difícil o imposible de realizar. En este trabajo se realizó un análisis retrospectivo mediante IHQ en 19 muestras de pulmón e hígado, en cada caso, de fetos bovinos con diagnóstico previo de C. fetus por cultivo y/o IFD y con lesiones histopatológicas compatibles. Se empleó un kit de IHQ de alta sensibilidad y dos métodos de recuperación antigénica. Las lesiones histopatológicas más relevantes en los fetos fueron bronconeumonía supurativa o mixta (16/19), serositis no supurativa a nivel del intestino y pericardio (14/19), hepatitis periportal (14/19) y meningitis no supurativa (10/19). La inmunomarcación fue positiva a C. fetus en la totalidad de los pulmones (19/19) y negativa en todos los hígados (19/19) procesados; independientemente del método de recuperación antigénica. En base a estos resultados, suponemos que algún factor inherente al hígado fetal estaría actuando en detrimento de la IHQ para la identificación de C. fetus. Se demuestra que el pulmón es el órgano de elección para la inmunomarcación de C. fetus en los fetos bovinos abortados.Bovine campylobacteriosis is an infectious disease that causes infertility and abortions in ruminants. The main species involved are Campylobacter fetus spp. venerealis (CFV), Campylobacter fetus spp. fetus (CFF) and Campylobacter jejuni. Diagnosis of abortion due to campylobacteriosis in fetuses is made by isolation of the bacteria in special cultures and by direct immunofluorescence (DFAT) from abomasal content and lung. Immunohistochemistry (IHC) is another diagnostic tool that allows retrospective studies and precise association of the antigen with the lesions. Likewise, this technique is relevant when fetuses are depredated, autolytic and/or contaminated, since bacteriological culture is difficult or impossible to perform. In this work, a retrospective analysis by IHC was performed on 19 lung and liver samples, in each case, from bovine fetuses with previous diagnosis of C. fetus by culture and compatible histopathological lesions. In contrast to previous studies, a highly sensitive IHQ commercial kit and two antigenic recovery methods were used. The most relevant histopathological lesions in the fetuses were suppurative or mixed bronchopneumonia (16/19), non-suppurative serositis at the level of the intestine and pericardium (14/19), non-suppurative periportal hepatitis (14/19) and meningitis (10/19). Immunolabeling was positive for C. fetus in all lungs (19/19) and negative in all livers (19/19) processed; regardless of the antigenic recovery method and IHC kit employed. Based on these results, we assume that some factor inherent to the fetal liver would be acting to the detriment of the IHC technique for the identification of C. fetus. It is demonstrated that the lung is the organ of choice for C. fetus immunolabeling in aborted bovine fetuses.EEA BalcarceFil: Scioli, Maria Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentin
Failure to establish infection with Tetratrichomonas sp. in the reproductive tracts of heifers and bulls
Full text linkExperimental infection of the reproductive tracts of heifers and bulls with Tetratrichomonas sp. isolated from preputial smegma of virgin bulls was attempted. Nine heifers and four bulls were challenged by inoculation of 7×106 Tetratrichomonas sp. into the vaginal lumen and preputial cavity, respectively. Vaginal mucus and preputial smegma samples were collected and cultured for Tetratrichomonas sp. Heifers were slaughtered in groups of three at 2, 9 and 21 days after inoculation. Two heifers and two bulls infected with Tritrichomonas foetus and two uninfected heifers were used as controls for the model infection. Tetratrichomonas sp. were only isolated in vaginal mucus of 7/9 inoculated heifers at 6 h post-inoculation, and genital secretions taken at slaughter time from vagina, uterus and oviduct were cultural negative. Bulls challenged with Tetratrichomonas sp. remained cultural negative. Since Tetratrichomonas sp. survived only a few hours in the female genitalia and did not survive in the male genitalia after experimental challenge, Tetratrichomonas sp. did not colonize the genital tract. These were likely trichomonads from the digestive tract. Collection of clean samples without fecal contamination from the reproductive tract is proposed as a measure to avoid Tetratrichomonas sp. transitory genital infection.Fil: Cobo, Eduardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Canton, German. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Cano, Dora. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentin
Spontaneous outbreak of Pascalia glauca poisoning in sheep in Argentina
No full textPascalia glauca is a native weed and one of the most common hepatotoxic plant affecting cattle in Argentina. Although experimental P. glauca poisoning have been reported in sheep, no spontaneous cases have been reported in this species. This work describes an outbreak of intoxication after spontaneous consumption of P. glauca, affecting 20% (6/30) sheep of a commercial flock. Affected sheep were ataxic, depressive, with mucous nasal discharge, cough and abdominal breathing. During post mortem examination, liver was swollen and a diffuse enhancement of the reticular pattern (“nutmeg liver”) was visible. Histopathological examination reveals severe and extensive acute diffuse centrilobular hepatic necrosis with hemorrhage. The presence of the toxic plant, the clinical and pathological findings allows us to confirm the etiology of this outbreak.EEA BalcarceFil: Liboreiro, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Flores Olivares, Carlos. Universidad Pedro de Valdivia, Medicina Veterinaria; Chile.Fil: Armendano, Joaquín. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Schild, Carlos. Instituto Nacional de Investigación Agropecuaria (INIA); Uruguay.Fil: Fernández, Eduardo Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Odriozola, Ernesto Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Resumen Sanitario: Servicio de Diagnóstico Veterinario Especializado [4° Trimestre 2018]
No full textDesde fines de septiembre hasta fines de diciembre del 2018 ingresaron al Servicio de Diagnóstico Veterinario Especializado (SDVE) del INTA EEA Balcarce, 157 protocolos que incluyeron muestras enviadas por asesores y laboratorios de diagnóstico privados, así como viajes de diagnóstico (14 en total, recorriendo 9400 km). Se realizaron 30 necropsias. A continuación se resumen los casos de diagnóstico más relevantes registrados por el SDVE durante este periodo.EEA BalcarceFil: Acuña, Joaquin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; ArgentinaFil: Canton, German Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; ArgentinaFil: Fanti, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; ArgentinaFil: Garcia, Joaquin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; ArgentinaFil: Gómez, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; ArgentinaFil: Llada, Ignacio Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; ArgentinaFil: Lobo, Juan Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; ArgentinaFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; ArgentinaFil: Soteras, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; ArgentinaFil: Urtizbiria, Facundo Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Servicio de Diagnóstico Veterinario Especializado; Argentin
Enteric listeriosis in grazing steers supplemented with spoiled silage
Full text linkAn outbreak of enteric listeriosis in steers that were fed spoiled silage is reported. The outbreak started 2 days after ~200 animals in a single paddock were given a supplement of spoiled silage. Forty animals (20%) were affected, and 13 (6.5%) died over a period of 10 days. Affected animals were recumbent, depressed, and had diarrhea with mucus and fibrin. Gross and microscopic findings in 3 animals that were subjected to autopsy included excess peritoneal fluid, congestion and edema of abomasum, suppurative enteritis and colitis, and suppurative mesenteric lymphadenitis. Two strains of Listeria monocytogenes were isolated, one of serotype 1/2c from the gallbladder and one of serotype 1/2b from the spoiled silage. Listeria monocytogenes was detected in the mesenteric lymph nodes and intestinal wall of 1 animal by immunohistochemistry (IHC). Clinical history and signs, gross and microscopic findings, bacterial isolation, and IHC results confirmed a diagnosis of enteric listeriosis. The source of infection was likely the spoiled silageEEA BalcarceFil: Garcia, Juan Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina.Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Salud Animal; ArgentinaFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Salud Animal; ArgentinaFil: Odriozola, Ernesto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Salud Animal; ArgentinaFil: Moreira, Ana Rita. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Salud Animal; Argentin
Systemic granulomatous disease in dairy cattle from Argentina
Full text linkAn outbreak of systemic granulomatous disease of unknown etiology was diagnosed in a dairy herd from Argentina. Eleven out of 211 cows manifested hyperthermia, depression, alopecia, pruritus, decreased milk production and death in most cases. During necropsy, multifocal petechial hemorrhages in glottis and vulva, white multifocal nodules in the liver and splenomegaly with subcapsular petechial hemorrhages were observed. Both kidneys were diffuse pale and enlarged. Systemic granulomatous hepatitis, myocarditis, pancreatitis and nephritis were observed. Water and food samples tested negative for Arsenic and T2 toxin, respectively. Fusarium equiseti was isolated from alfalfa hay samples. Vicia spp. was not consumed by the affected herd and no other cause of vetch-like disease was registered. Other causes of granulomatous lesions (Mycobacterium spp. and fungal infections) were discarded. The systemic granulomatous disease was suggestive of a type IV hypersensitivity reaction. Although the sensitizing agent was not determined, two components of the ration were suspected: cotton seed and bone ash. Both of them were introduced one month prior to the detection of the first affected cow and the disease resolved since they were removed from the diet.EEA BalcarceFil: Odriozola, Ernesto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Dorsch, Matías. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Caffarena, Rubén Darío. Instituto Nacional de Investigación Agropecuaria (INIA). Plataforma de Investigación en Salud Animal. Universidad de la República. Facultad de Veterinaria; Uruguay.Fil: Moreira, Ana Rita. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Fernández, Eduardo Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Dictiocaulosis en vacas de cría en la provincia de Buenos Aires, Argentina: descripción de dos brotes = Dictyocaulosis in beef cows in Buenos Aires province, Argentina: description of two outbreaks
No full textLa dictiocaulosis o bronquitis verminosa es ocasionada por Dictyocaulus viviparus, un nematode de ciclo
de vida directo, y está asociada, mayormente, a brotes de enfermedad clínica en bovinos jóvenes en su
primera temporada de pastoreo. El presente trabajo describe dos brotes de bronquitis verminosa que
provocó la mortandad de entre el 13,6 % y el 15,7 % de dos rodeos de vacas de cría de establecimientos de la
provincia de Buenos Aires, Argentina. En ambos brotes, los hallazgos clínicopatológicos en los animales
afectados y la presencia de nematodes pulmonares, en algunos casos solo observados en el estudio
histopatológico, permitieron confirmar este diagnóstico.
Dictyocaulosis or parasitic bronchitis is caused by Dictyocaulus viviparus, a direct life cycle nematode, and
is most frequently associated with clinical disease in young cattle during their first grazing season. This
work describes two outbreaks of dictyocaulosis, in two cowcalf farms in Buenos Aires province, Argentina
that caused the death of 13.6 % and 15.7 % of animals, respectively. In both outbreaks, clinical and
pathological findings, and the presence of nematodes in respiratory tract, in some cases only observed in
the histopathological analysis, allowed diagnosis confirmation.EEA BalcarceFil: Llada, Ignacio Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Sicalo Gianechini, Leonor. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Lloberas, María Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Odriozola, Ernesto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Canton, German Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentin
Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
Full text linkBovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the basis of histopathological, immunohistochemical, lectinhistochemical and polymerase chain reaction (PCR) techniques. The most frequent foetal lesion was neutrophilic bronchopneumonia and interstitial pneumonia. Other commonly observed lesions included non‐suppurative interstitial enteritis, hepatitis, pericarditis, myositis, myocarditis, and meningitis. In this study, C. fetus fetus was phenotypically classified in all bovine foetuses from lungs and abomasal fluids. Immunohistochemistry (IHC) staining revealed positive stained Campylobacter organisms with typical morphology. Lectin binding patterns not showed great differences between the infected and the non‐infected groups. The most important changes were a minor peanut agglutinin (PNA) and DBA binding in the alveolar cells of the lungs and DBA globet cells in some of the C. fetus–positive foetuses. Individual variations in each lectin binding pattern complicate the evaluation of the lectins results. All foetuses positive to IHC were positive by PCR. Better efficiency of PCR was obtained from abomasal fluids than from lung tissues. The association of culture and phenotypic techniques with histopathology, IHC and PCR allowed a better characterization and description of BC.EEA BalcarceFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; ArgentinaFil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentin
Investigating the mechanism of action and effects of polyacrylamide hydrogel (Arthramid Vet) on the joints of Thoroughbred horses
No full text© 2025 Eleonora Lidia MorrellOsteoarthritis (OA) is responsible for a considerable number of lameness cases in horses, mainly in the metacarpophalangeal and carpal joints; causing chronic pain, poor performance, and early retirement from racing. In racing Thoroughbreds, as well as OA being a common problem, osteochondral disease is very commonly found in the metacarpophalangeal (fetlock) and carpal joints (fetlock osteochondral disease is known as palmar or plantar osteochondral disease, POD). Synovial changes are an important factor in the pathophysiology of OA, but the relationship between osteochondral disease and synovitis in racing Thoroughbreds is unclear. Intra-articular corticosteroids are commonly used in the treatment of equine OA, but now polyacrylamide hydrogel (PAHG) has received significant recent interest as a novel treatment in horses; however, the mechanism of action remains unclear. The aims of this study were to characterise and grade the histologic changes in the synovium of the fetlock, carpal and tarsal joints of Thoroughbred racehorses; and to examine their association with gross lesions including the presence of osteochondral disease. Regarding PAHG, the objectives were to characterize the synovial changes associated with administration of PAHG in joints with OA and determine the likely mechanism(s) by which PAHG may reduce joint pain and/or inflammation.
A retrospective study was performed on post mortem samples from horses euthanased for reasons unrelated to the present study. The fetlock, carpal and tarsal joints of 54 Thoroughbred racehorses were examined post mortem and gross changes including the presence of POD. Synovial membranes of the same joints were examined histologically and a semi-quantitative scoring and grading system was developed. The sensory nerves and type A synoviocytes in the synovium of joints from horses administered PAHG were examined to investigate changes to inflammatory cell populations and look for any reduction of sensory nerves. Furthermore, the direct and indirect effects of PAHG on cultured neurons and macrophages were examined.
The main gross lesion in the forelimb fetlock joints of Thoroughbred horses at post mortem was POD, including cartilage loss and cartilage wear lines, while the main gross changes in the hindlimb fetlock joints were the presence of cartilage linear fissures and cartilage loss. The total histopathological score of the synovium from carpal joints was significantly higher than the total score of the forelimb fetlock joints. The total synovitis score was not associated with the total gross score for POD in the forelimb fetlock joints. Where horses had received PAHG treatment, intimal thickening and macrophage infiltration into the subintima was observed. Between 2-9 months post treatment, PAHG was no longer observed in the synovium. There was no correlation between number, size or quantity of synovial sensory nerves and time since PAHG injection. To confirm that there were no direct or indirect toxic effects of PAHG on neurons, equine and rat neurons were cultured in vitro and co-incubated with PAHG and also supernatants from macrophages treated with PAHG. No toxic effects of PAHG on neurons were observed. Since macrophages were seen to play a key role in the tissue responses to PAHG injections in vivo, the effects of this compound were examined more closely in cultured equine macrophages in vitro. PAHG induced equine macrophages to produce the anti-inflammatory cytokine, IL-10, consistent with the adoption of the M2 phenotype.
These findings suggest that while synovitis may play an important role in OA, and osteochondral disease may eventually lead to OA, synovitis is not an inherent feature of osteochondral disease, including POD. PAHG does not appear to cause direct toxic effects to neurons. But after it is taken up into the synovium by macrophages and type A synoviocytes, this material may induce the anti-inflammatory M2 phenotype with the release of IL-10 and other anti-inflammatory factors, which reduces the stimulation of sensory nerves. Overall, these findings may have implications for the treatment and management of OA and osteochondral disease in horses, as well as understanding the causes of pain and lameness. Further studies are required to confirm the mechanism of action of PAHG in vivo; and investigate comparisons between PAHG and intra-articular corticosteroids in different presentations of OA and joint pain
Spongy Myelinopathy in Newborn Beef Calves Associated with Consumption of Corn Infected with Stenocarpella maydis
No full textStillbirth and perinatal mortality with neurological signs and lesions were diagnosed in two calves following ingestion by their dams of corn infected with Stenocarpella maydis during the third trimester of gestation. Grossly, the brain and spinal cord were unremarkable. Microscopically, diffuse severe status spongiosis of the white matter was detected in the cerebral hemispheres, brainstem, spinal cord and cerebellum. To the best of our knowledge this is the first pathological description of congenital disease in calves associated with the consumption of S. maydis-infected corn; the findings resemble those reported for the naturally occurring and experimentally induced disease in lambs.EEA BalcarceFil: Odriozola, Ernesto Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Armién, Aníbal Guillermo. University of Minnesota. Veterinary Diagnostic Laboratory; Estados UnidosFil: Cora Ibarra, Juan Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Llada, Ignacio Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Erreguerena, Ignacio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
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