1,721,360 research outputs found
Dataset for: High-Speed Single-Cell Dielectric Spectroscopy
No full textDataset for: Spencer, Daniel and Morgan, Hywel. (2020) High-Speed Single-Cell Dielectric Spectroscopy. ACS Sensors. DOI: https://doi.org/10.1021/acssensors.9b02119</span
AC and phase sensing of nanowires for biosensing
No full textData supporting the paper "Crescentini, Marco, Rossi, Michele, Ashburn, Peter, Lombardini, Marta, Sangiorgi, Enrico, Morgan, Hywel and Tartagni, Marco (2016) AC and phase sensing of nanowires for biosensing. Biosensors, 6, (2), 1-14. (doi:10.3390/bios6020015)"</span
A low-noise transimpedance amplifier for BLM-Based ion channel recording
No full textData for the paper "Crescentini, Marco, Bennati, Marco, Saha, Shimul C., Ivica, Josip, de Planque, Maurits R.R., Morgan, Hywel and Tartagni, Marco (2016) A low-noise transimpedance amplifier for BLM-based ion channel recording. Sensors, 16, (5, 709), 1-20. (doi:10.3390/s16050709)."</span
Dataset supporting the publication "Concentration–polarization electroosmosis for particle fractionation"
No full textThis dataset supports the publication in the journal Lab on a Chip entitled “Concentration Polarization Electrophoresis for particle fractionation” (2024) DOI: https://doi.org/10.1039/D4LC00081A
This is the original data used to create Figures 2 and 4 in the above paper. We also include an example of original unprocessed video recording used to evaluate the fractionation of S. Aureus bacteria from 3 micron carboxylate particles, showing both populations flowing at the end of the devices in a 1.5 mS/m KCl electrolyte. Particles are subjected to an AC electric field of 100 kV/m amplitude and a frequency of 1.7 kHz</span
Microfluidic impedance cytometry-measuring single cells at high speed
No full textHigh throughput single cell microfluidic analysis platforms offer the ability to characterize large numbers of individual cells (or more generally particles) at high speed. Miniature flow cytometers offer new methods for the rapid analysis of single cells. Impedance analysis of single cells provides information on cell size (volume), membrane and cytoplasmic characteristics. The technology has developed rapidly and offers the prospects of new approaches for counting and differentiating cells with applications from basic research to point of care diagnostics
AC electrokinetic particle manipulation in microsystems
No full textLab-on-Chip systems integrate multiple functionalities on a single platform. Automated or remote manipulation and analysis of particles and fluids is a key element in microfluidic devices. Microelectrodes can be integrated into these devices to generate large electric fields and field gradients using low voltages. Electrokinetics is an attractive method for integrating particle manipulation and separation within such systems. The electrokinetic forces are easy to control by designing optimum electrode structures and choice of field and frequency. In this chapter, the theory of AC electrokinetics is reviewed and example applications for manipulation of particles are provided. The use of dielectrophoresis (DEP) for manipulating micro particles is then described, together with a discussion on scaling issues
Single cell microfluidic impedance cytometry – a review
No full textLab on chip technologies are being developed for multiplexed single cell assays. Impedance offers a simple non-invasive method for counting, identifying and monitoring cellular function. A number of different microfluidic devices for single cell impedance have been developed. These have potential applications ranging from simple cell counting and label-free identification of different cell types or detecting changes in cell morphology after invasion by parasites. Devices have also been developed that trap single cells and continuously record impedance data. This technology has applications in basic research, diagnostics or non-invasively probing cell function at the single-cell level. This review will describe the underlying principles of impedance analysis of particles. It then describes the state of the art in the field of microfluidic impedance flow cytometry. Finally future directions and challenges are discussed
Microparticle encoding technologies for high-throughput multiplexed suspension assays
No full textThe requirement for analysis of large numbers of biomolecules for drug discovery and clinical diagnostics has driven the development of low-cost, flexible and high-throughput methods for simultaneous detection of multiple molecular targets in a single sample (multiplexed analysis). The technique that seems most likely to satisfy all of these requirements is the multiplexed suspension (bead-based) assay, which offers a number of advantages over alternative approaches such as ELISAs and microarrays. In a bead based assay, different probe molecules are attached to different beads (of a few tens of microns in size), which are then reacted in suspension with the target sample. After reaction, the beads must be identifiable in order to determine the attached probe molecule, and thus each bead must be labelled (encoded) with a unique identifier. A large number of techniques have been proposed for encoding beads. This critical review analyses each technology on the basis of its ability to fulfil the practical requirements of assays, whilst being compatible with low-cost, high-throughput manufacturing processes and high-throughput detection methods. As a result, we identify the most likely candidates to be used for future integrated device development for practical applications
Single cell impedance spectroscopy
No full textCellular analysis requires a combination of biophysical and biochemical approaches for counting, manipulation and characterization of biological cells. In recent years, considerable attentions have been paid to single cell analysis based on Lab-On-a-Chip (LOC) technology, which offers the characterization of a large amount of cells one by one [1-3]. Electrical impedance spectroscopy (EIS) provides a high speed, non-invasive and label-free technique for single cell analysis. We have fabricated microfluidic chips with integrated microelectrodes inside the microchannel to perform a differential impedance measurement, as shown in figure 1. Two pairs of parallel facing electrodes define a detection and reference volume. AC excitation voltages at mixed frequencies are applied to the microelectrodes, generating electric field in the channel. As a cell passes by, it modifies the current lines through each of the two detection volumes in turn. A positive and negative peak variation in the measured differential current signal can be observed. Due to differences in the dielectric properties and sizes of various cells, specific information can be obtained from a single cell membrane, cytoplasm or nucleus at distinct frequencies
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