2,482 research outputs found
Abstract 147: Identification of cell sub-populations in Neuroblastoma cell lines with single-cell-sequencing analysis
No full textAbstract
Neuroblastoma is the most frequent extracranial tumor in neonatal patients. Engineering SKNBE2 Myc-N amplified Neuroblastoma cell lines we generated individual clones expressing NDM29 non-coding-RNA at different levels. Using these cell models we documented a specific role of NDM29 in tumor cell differentiation leading to the identification of novel genes to be pharmacologically targeted for an efficient control of tumor malignancy (CA9 and MCM2 genes). Further studies taking advantage of the same models suggested the possible co-existence of different cell sub-populations in the apparently homogeneous cell culture with possible different roles in tumor maintenance and growth. Here we test this hypothesis by performing single-cell sequencing analysis and identified different sub-populations on the base of their gene expression patterns. The characterization of their specific profiles of membrane markers allowed an efficient separation of the individual populations by FACS analysis. Our preliminary results show the co-existance of cells with a marked neural phenotype with other two populations one VEGF secreting and the other VEGF recipient/responder. These preliminary findings suggest further detailed investigations of possible paracrine loops that might be at the base of tumor cell nodule growth and maintenance.
Citation Format: Michele Tomanelli, Davide Ceresa, Adriana Bajetto, Paola Modesto, Paolo Malatesta, Tullio Florio, Aldo Pagano. Identification of cell sub-populations in Neuroblastoma cell lines with single-cell-sequencing analysis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 147
Filologia editoriale, Roberto Calasso in dialogo con Paola Italia e Francisco Rico
No full textPaola Italia e Francisco Rico intervengono sul libro di Roberto Calasso, presidente e fondatore di Adelphi Edizioni, L'impronta dell'editore, e discutono di problemi di filologia delle forme editoriali, dal punto di vista dell'autore, del lettore e dell'editore.Paola Italia and Francisco Ricos interview Roberto Calasso, Publisher, Writer, and Founder of Adelphi Edizioni, about his book: L'impronta dell'editore, talking about philology, publishing and editing, from the author, the reader and the publisher's point of view
Paola Gianturco: Women Who Light the Dark
No full textPaola Gianturco is a photographer, author, and advocate for women\u27s rights world-wide. For the past thirteen years, she has worked as a photojournalist, documenting women’s lives in forty countries. She has published four acclaimed photo books which bring together inspiring stories with gorgeous photographs to motivate her readers to engage with, learn from and support women around the world. All of Gianturco’s books are philanthropic projects, for which she donates her royalties to carefully selected nonprofit organizations that relate to each book\u27s content. Paola\u27s most recent book, Women Who Light the Dark, tells the story of local women around the world who are helping one another tackle the problems that darken their lives—including violence, poverty, illiteracy and disease. Gianturco is giving 100% of her author royalties for this book to The Global Fund for Women.https://thekeep.eiu.edu/humanitiescenter_meaningfulwork1011/1002/thumbnail.jp
Identification of Bifidobacteria by the Phosphoketolase Assay
No full textBifidobacteria are commensal bacteria, which naturally colonize the gastrointestinal tract of a large number of animals, including humans, contributing to their health and well-being. An important taxonomic marker for the identification of members of the bifidobacterial group is the presence of the fructose-6-phosphate phosphoketolase (F6PPK) activity. The F6PPK enzyme is involved in the bifidus shunt based on the ability of F6PPK to split fructose-6-phosphate into erythrose-4-phosphate and acetyl phosphate. Here, we describe the two main methods utilized to detect the presence of F6PPK activity, that is, the enzymatic assay and the presence of the D-xylulose-5-phosphate/fructose-6-phosphate phosphoketolase bifidobacterial gene
Resistance to freezing and freeze-drying storage processes of probiotic bifidobacteria.
No full textThe resistance to freezing at –135 °C and freeze-drying of potentially probiotic
bifidobacteria strains isolated from human habitat and from commercial probiotic products
already present in the Italian market were tested. The analysis was conducted in two
different cryoprotectives, sucrose and skim milk. The strains of Bifidobacterium animalis, the only species found in the commercial probiotic product, were resistant to freezing and freeze-drying in both of the cryoprotectives used. Bifidobacteria from human habitat, B 1501 and B 632 Bifidobacterium breve strains and B 600/2, B 669/3 and B 2416 Bifidobacterium catenulatum strains, resulted as the most resistant strains in the storage processes in both cryoprotectives. These strains are potentially useful for industrial applications. In the strains tested the percentage of viable cells were higher after freezing than after freeze drying. The performance of the cryoprotectives analysed were strain-dependent
«Yo soy de Destierrolandia»: Fernando Arrabal y el límite
No full textFernando Arrabal is a Spanish author - actually, no, he isn't. He is a francophone writer, or, more accurately, a Spanish expatriate. Or, on the contrary, he is a traitor, an «afrancesado». In this paper Fernando Arrabal's case will be used as an example of the difficulties that an author has to face when he leaves his own country and moves to another different from a spatial, cultural, and linguistic point of view. Besides, I will analyze the unusual position of the critic in the reception and interpretation of a corpus that, while being obviously connected to its author, cannot refer to a precise national context
Approcci integrati a base di probiotici e sostanze naturali per la salute del nostro microbiota. Studi in vitro e in vivo.
No full textE’ noto come il corpo umano sia colonizzato da un gran numero di microrganismi che coesistono pacificamente con il l’organismo ospite e si accentrano principalmente nel colon, dove si stima la presenza del 70% dei microrganismi presenti in tutto il corpo.
Una vasta letteratura mostra come il microbiota sia in grado di: influenzare e mantenere l’omeostasi del sistema immunitario, agire come barriera per l’invasione di patogeni e sostenere l’ospite grazie alla produzione di composti nutrizionali. La variazione della composizione del microbiota, generalmente nota come disbiosi, può distruggere questa interazione mutualistica e influenzare negativamente la fisiologia dell’ospite compromettendone lo stato di salute. Disbiosi intestinali sono strettamente legate ad importanti patologie umane incluso le patologie infiammatorie e autoimmuni, come le IBD (Intestinal Bowel Disease) e IBS (Intestinal Bowel Syndrome), metaboliche, tra cui l’obesità e il diabete, e neurologiche. Pertanto, durante la vita di ciascun individuo, il microbiota può essere soggetto a insulti in grado di portare ad alterazioni importanti della fisiologia dell’organismo e quindi dello suo stato di salute.
Negli ultimi anni, il nostro gruppo ha condotto linee di ricerca tese a valutare l’efficacia di interventi a base di sostanze naturali nel riequilibrio del microbiota vaginale e intestinale. Quanto detto al fine di aiutare l’organismo a ristabilire il corretto equilibrio, e conseguentemente le fisiologiche difese dell’organismo, per contrastare patologie croniche fungine, come le candidiasi recidivanti vaginali (CVVR), e gastro-intestinali, come le IBD.
I nostri risultati mostrano come approcci terapeutici combinati, caratterizzati da probiotici e ovuli vaginali a base di tea tree oil, siano interessanti per contrastare le CVVR e come, in un futuro prossimo, sostanze naturali quali gli oli essenziali del genere Citrus e/o gli idrolati di Monarda spp, Citrus spp e Vitis vinifera potranno essere considerate nel tentativo di ristabilire il corretto equilibrio microbico intestinale in patologie come le IBD
Introduzione, a Mario Tobino, Il Clandestino
No full textL'introduzione presenta il libro più ambizioso di Mario Tobino, Il Clandestino, dedicato al racconto della sua esperienza con i gruppi clandestini della Resistenza Viareggina, libro con cui l'autore vinse il Premio Strega, nel 1962, imponendosi all'attenzione del grande pubblico dopo il successo dei libri manicomiali. Una nuova edizione in cui Paola Italia valorizza i materiali inediti dell'Archivio Tobino conservato presso l'Archivio Contemporaneo A. Bonsanti del Gabinetto GP Vieusseux di Firenze,Paola Italia presents an Introduction to the new edtion of Il Clandestino, the most ambitious of Mario Tobino's novels, dedicated to the story of his experience with the groups of the Tuscan Resistance when he was a psichiatric doctor ar Lucca. With this book the author won the Strega Prize, in 1962, attracting the attention of a wide public after the success of his psichiatric books. A new edition where Paola Italia enhances the unpublished material of Tobino Archive preserved in A. Bonsanti Contemporary Archive of G.P. Vieusseux Cabinet of Florence
Gut microbiota and its pivotal role in the welfare of the animals
No full textThe gut microbiota has been reported to have a pivotal role in physiological functions in vertebrates. Many studies discuss the functional implications of the gut microbiota not only on immunity, growth, and metabolism but also on brain development and behavior. However, while the influence of the microbiota-gut-brain axis on behavior is documented in rodents and humans, data on wild and zoo-housed animals are scarce. There are few studies regarding
animals’ health and welfare status concerning the gut microbiota (higher alpha diversity, beneficial composition, and positive functions). The gut microbiota is effectively influenced by health characteristics, immunity, behaviors, and stress response in animals, which would provide a novel approach to measure and evaluate the health status and welfare of animals. It is concluded that the gut microbiota can be scientifically used to assess and improve the
welfare of animals. The gut microbiota is a promising tool to evaluate and improve animal welfar
Expanding our knowledge on bifidobacteria in primates
No full textThe aim of th epresent study is to describe the bifidobacterial species biodiversity in non-human primates
in gut of: marmoset, tamarins, saimiri, saki, gelada, cercopitecae, macaque and lemur
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