112,098 research outputs found
Modificazioni dell'attività profilerativa nel tubo neurale toracico dell'embrione di pollo conseguente a trapianto di un abbozzo di coda
No full text
Cardiotoxicity of the Helicobacter pylori lipopolysaccharide to the embryo
No full textHelicobacter (H.) pylori is the causative agent of the peptic ulcer disease and a co-factor in the development of gastric malignancies. Recently, it has been maintained that chronic H. pylori infections in adults are linked to a higher risk of coronary heart diseases. In this respect, the acute toxic effects of the H. pylori lipopolysaccharide (LPS) on embryonal cardiomyocytes at different developmental stages was evaluated. White Leghorn chick embryos and smooth (S)--form NCTC 11637 strain H. pylori organisms were used. Both whole heath-killed H. pylori suspensions (3.10(6) bacteria/egg) and isolated S-LPS (500 ng/egg) or S-Lipid A (500 ng/egg) were non-lethal to 4-day embryos, becoming moderately lethal (5% to 30%) to 6- and 8-day embryos and highly lethal (> 90%) to 10- to 17-day embryos. The contractile activity of isolated atrial fragments from 10-day embryos was completely inhibited, within 5 min, following treatments with heath-killed H. pylori (3 x 10(6)/ml), or S-LPS (500 ng/ml), or S-Lipid A (500 ng/ml); the block determined by S-LPS and S-Lipid A was irreversible, while the block by bacterial suspensions was completely reversible upon withdrawal. Following a 24-hour treatment with S-LPS or S-Lipid A of single-cell cultures of cardiomyocytes (isolated from 10-day embryos) a dose-dependent cell loss was observed, as assessed by total protein dosage and direct counting of adherent cells. Propidium Iodide/Annexin V FACS-analysis confirmed the occurrence of cellular necrosis, but did not show any evidence of apoptotic processes. The release of superoxide anion radicals by cultured cardiomyocytes was as follows: S-Lipid A (25 micrograms/ml) > S-LPS (25 micrograms/ml) > heath killed H. pylori suspensions (3 x 10(6)/ml); control cultures did not release detectable amounts of superoxide anion radicals. Furthermore, cultured cardiomyocytes produced increased amounts of NO (N-monomethylarginine-inhibitable) following stimulation with S-LPS (25 micrograms/ml) or S-Lipid A (25 micrograms/ml) (but not heath killed H. pylori 3 x 10(6)/ml suspensions). Under all the above experimental conditions S-polysaccharide proved to be non-toxic. Concluding, H. pylori LPS is relatively non-toxic to the less differentiated cardiomyocytes; cardiomyocytes which are more advanced in their biochemical differentiation become highly sensitive to LPS and produce ROS and NO. ROS are probably responsible for the early toxic actions, while both ROS and NO are likely to be involved in the later degenerative/necrotic effects
Inhibition of protein kinase C delta cleavage following treatment of human monocytic cell line U-937 with lipophosphoglycan of Leishmania infantum
No full textInhibition of caspase 3 in human monocytic cell line U 937 in vitro infected with Leishmania infantum
No full textI Charge, Therefore I Drive: Current State of Electric Vehicle Charging Systems
No full textI charge, therefore I drive. Is this the new paradigm? The ambitious goals set by governments worldwide to phase out gasoline-powered cars are the driving force behind the upgrade of electric vehicle (EV) charging infrastructures. The EV industry and roadways departments may be asked to invest more in wireless charging, that is, without the need for any cable. Transmitter pads that can charge the EV in the garage, or even briefly at traffic lights, may convey the stationary wireless charge, whereas coils embedded into roadways may power EVs as they drive overhead. The standardized wireless charge appears to be the optimum solution to placate the anxiety of drivers
Mutation, selection, and functional repair in formyl peptide receptor genes: a view on the selection processes occurring in this gene subfamily.
No full text- …
