1,411 research outputs found
Peer Interview Script, Danielle Mitchell, Spring 2020
No full textDanielle Mitchell is a rising senior from Compton, California majoring in anthropology and sociology. She is a gifted writer who conducted very special interviews in SIS Seminar
In the Garden, Danielle Mitchell, Spring 2020
No full textDanielle Mitchell is a rising senior from Compton, California majoring in anthropology and sociology. She is a gifted writer who conducted very special interviews in SIS Seminar
Hall Street, Danielle Mitchell, Spring 2020
No full textDanielle Mitchell is a rising senior from Compton, California majoring in anthropology and sociology. She is a gifted writer who conducted very special interviews in SIS Seminar
Excerpts of Interviews with Peers, Danielle Mitchell, Spring 2020
No full textDanielle Mitchell is a rising senior from Compton, California majoring in anthropology and sociology. She is a gifted writer who conducted very special interviews in SIS Seminar
Orchestration of Adaptive T Cell Responses by Neutrophil Granule Contents
No full textNeutrophils are the most abundant leukocytes in peripheral blood, and respond rapidly to danger, infiltrating tissues within minutes of infectious or sterile injury. Neutrophils were long thought of as simple killers, but now we recognise them as responsive cells able to adapt to inflammation and orchestrate subsequent events with some sophistication. Here, we discuss how these rapid responders release mediators which influence later adaptive T cell immunity, through influences on DC priming and directly on the T cells themselves. We consider how the release of granule contents by neutrophils – through NETosis or degranulation - is one way in which the innate immune system directs the phenotype of the adaptive immune response.<br/
How to write a novel - four fiction writers on Danielle Steel's insane working day
No full textFirst paragraph: She might be the world’s most famous romance writer, nay the highest selling living author bar none, but there’s little room for flowers and chocolates in Danielle Steel’s writing regime. In a recent interview she laughed at the idea of young people insisting on a work-life balance, and has claimed she regularly writes for 20 to 22 hours a day, and sometimes 24. The result: 179 books in under 50 years, selling about 800m copies.https://theconversation.com/how-to-write-a-novel-four-fiction-writers-on-danielle-steels-insane-working-day-11715
The Outcome of Neutrophil-T Cell Contact Differs Depending on Activation Status of Both Cell Types
No full textNeutrophils and T cells exist in close proximity in lymph nodes and inflamed tissues during health and disease. They are able to form stable interactions, with profound effects on the phenotype and function of the T cells. However, the outcome of these effects are frequently contradictory; in some systems neutrophils suppress T cell proliferation, in others they are activatory or present antigen directly. Published protocols modelling these interactions in vitro do not reflect the full range of interactions found in vivo; they do not examine how activated and naïve T cells differentially respond to neutrophils, or whether de-granulating or resting neutrophils induce different outcomes. Here, we established a culture protocol to ask these questions with human T cells and autologous neutrophils. We find that resting neutrophils suppress T cell proliferation, activation and cytokine production but that de-granulating neutrophils do not, and neutrophil-released intracellular contents enhance proliferation. Strikingly, we also demonstrate that T cells early in the activation process are susceptible to suppression by neutrophils, while later-stage T cells are not, and naïve T cells do not respond at all. Our protocol therefore allows nuanced analysis of the outcome of interaction of these cells and may explain the contradictory results observed previously
Innate modifiers of T cell behaviour during inflammatory disease
No full textNeutrophils are the most abundant leukocyte in mammals and represent one of the first lines
of defence against invading microorganisms. In recent years, it has become clear that
neutrophils are not only responsible for the killing of pathogens, but that they also play an
important role in shaping adaptive immune responses. The aim of this project was to
determine the role of the neutrophil-derived host defence peptide, cathelicidin, in the
generation of Th17 responses during inflammation. Following inoculation with heat-killed
Salmonella typhimurium, cathelicidin knockout mice cannot produce IL-17 and show
increased IFNγ, whereas other cytokines are produced normally. Ex vivo, I show that
cathelicidin is a novel Th17/Tc17 differentiation enhancing factor, which acts directly on both
CD4+ and CD8+ T cells to increase their activation status, protect them from death and
concentration-dependently upregulate IL-17 production. Gene expression analysis revealed
that cathelicidin downregulates the expression of several Th1-related genes and upregulates
the aryl hydrocarbon receptor (AHR), a known regulator of Th17 differentiation. The addition
of an AHR antagonist to our in vitro cultures abolishes the boost to IL-17 production normally
induced by cathelicidin. I provide further evidence that suggests lymph node neutrophils are
the cellular source of cathelicidin, which are responsible for amplifying type-17 responses
during inflammation. These data contribute to our understanding of how lymph node
neutrophils influence developing adaptive immune responses with sophistication and
specificity
Conversations with Danielle Cronin, Philip Howard and Julian Thomas
No full textThis chapter focuses on the expanding civic role and challenges for investigative journalists using digital and social media. The chapter includes conversations with Danielle Cronin (national deputy editor of the Australian Broadcasting Corporation), as well as Professor Philip Howard (director of the Oxford Internet Institute), along with Distinguished Professor Julian Thomas (director of the ARC Centre of Excellence at RMIT University). They share their insights into setting an agenda of priorities for research and practice about public interest journalism. This chapter is an edited transcription of their conversations with the author, Dr Caryn Coatney, for a panel session sponsored by the Australian and New Zealand Communication Association.
- This chapter provides new material about the impact of social media, online audiences and automation on investigative journalism
Adding Spice to the Slog: Humanities in Medical Training.
No full textWriting from personal experience, physician and author Danielle Ofri asks what evidence is needed to justify trying to humanize medical training via the power of literature
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