2 research outputs found
ORIGINAL ARTICLE Kluai Homthong Banlad: (Banana Production in Banlad) Production Cost and Economic Rent
No full textABSTRACT An economic rent motivates investors, so objectives of research were to examine and to analyze appropriate production systems of Kluai Homthong in Banlad, to investigate the feasibility of a system of Kluai Homthong farming which makes a high return for growers. The population used as a study group in Banlad district in Tambon Thomrong, Raisathon, Tamlu and Khongkrajed was 81 farming households. The data from a questionnaire was used as a research tool. The cost and benefit ratios, and IRR were used for statistical analysis of the questionnaire. The discount rate bank was of 3% with a three year period of study. The study found that Tambon Thomrong gained higher returns with cost and benefit ratios of 01.46 and IRR of 42.78 %, following Khongkrajed with 01.39 and IRR of 31.42%, whereas Tamlu was 01.15 and IRR of 18.79% respectively. An unproductive production system in Raisathon due to IRR was a negative 00.92 but cost and benefit was 01.06. Organic fertilization, soil maintenance, and selling products through Banlad Agricultural Cooperative improved efficiency of production
Isolation and characterization of chitinase from soil fungi, Paecilomyces sp.
No full textAbstractChitinolytic fungal strains were isolated from soil in Thailand. They were screened as chitinase producers by testing their shrimp shell digestion ability on potato dextrose agar plates. The chitinase activity was tested with colloidal chitin in culture medium C and basal medium. There was greater activity in culture medium C than in the basal medium. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis from the culture filtrate of medium C showed three protein bands at about 40 kDa, 46 kDa and 56 kDa. The chitinase gene was sequenced from genomic DNA. The obtained sequence consisted of 713 bp upstream, a 1499 bp open reading frame that was interrupted by three introns and 1698 bp downstream sequences. The intron lengths were 63 bp, 57 bp and 110 bp, respectively. The sequence was found to be the most similar to the chitinase gene of Paecilomyces lilacinus (EF183511). Pairwise alignment of the 1499 bp and P. lilacinus resulted in 72.5% DNA sequence identity, while alignment of the 1269 bp coding sequence and P. lilacinus resulted in 78.5% cDNA sequence identity and 83.5% amino acid sequence identity. The protein structure contained two conserved domains of the putative substrate binding site (S-I-G-G) and catalytic domain (D-G-I-D-L-D-W-E), suggesting that this fungal chitinase belonged to the glycosyl hydrolases family 18 chitinase (GH18). Phylogenetic analysis of the chitinase gene from the nematopathogenic fungi suggested that this chitinase sequence was class V chitinase
