2,259 research outputs found
An alternative mechanism of clathrin-coated pit closure revealed by ion conductance microscopy
No full textCurrent knowledge of the structural changes taking place during clathrin-mediated endocytosis is largely based on electron microscopy images of fixed preparations and x-ray crystallography data of purified proteins. In this paper, we describe a study of clathrin-coated pit dynamics in living cells using ion conductance microscopy to directly image the changes in pit shape, combined with simultaneous confocal microscopy to follow molecule-specific fluorescence. We find that 70% of pits closed with the formation of a protrusion that grew on one side of the pit, covered the entire pit, and then disappeared together with pit-associated clathrin-enhanced green fluorescent protein (EGFP) and actin-binding protein-EGFP (Abp1-EGFP) fluorescence. This was in contrast to conventionally closing pits that closed and cleaved from flat membrane sheets and lacked accompanying Abp1-EGFP fluorescence. Scission of both types of pits was found to be dynamin-2 dependent. This technique now enables direct spatial and temporal correlation between functional molecule-specific fluorescence and structural information to follow key biological processes at cell surfaces
C.J. Koch (1932 - )
No full textBiographical, bibliographical, and literary historiography of Australian author C.J. Koch
Audio Interview with Mr. C.J. Rayner Whiteley
No full textAudio - Mr. Whiteley recounts the story of delivering the message of the flood of 1904. He took seventeen hours by horse to travel from Perryvale to Edmonton. Billy Loutit took the same message by foot arriving in Edmonton about the same time. Mr. Whiteley discusses early settlers, farming, homesteading and businesses. He has many anecdotes about life and people living in Athabasca at the start of the twentieth century. He freighted for ten years with the Hudson's Bay Company and also discusses early farming prices and technology extensivelyInformative Interview of Mr. C.J. Raymor Whitely In April 1961 on a Reel to Reel tape recorded onto cassette by R. Tanhas March 198
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Dominant components of the Thoroughbred metabolome characterised by 1H‐NMR spectroscopy: a metabolite atlas of common biofluids
No full textSummary
Reasons for performing study: Metabonomics is emerging as a powerful tool for disease screening and investigating mammalian metabolism. This study aims to create a metabolic framework by producing a preliminary reference guide for the normal equine metabolic milieu.
Objectives: To metabolically profile plasma, urine and faecal water from healthy racehorses using high resolution 1H-NMR spectroscopy and to provide a list of dominant metabolites present in each biofluid for the benefit of future research in this area.
Study design: This study was performed using seven Thoroughbreds in race training at a single time-point. Urine and faecal samples were collected non-invasively and plasma was obtained from samples taken for routine clinical chemistry purposes.
Methods: Biofluids were analysed using 1H-NMR spectroscopy. Metabolite assignment was achieved via a range of 1D and 2D experiments.
Results: A total of 102 metabolites were assigned across the three biological matrices. A core metabonome of 14 metabolites was ubiquitous across all biofluids. All biological matrices provided a unique window on different aspects of systematic metabolism. Urine was the most populated metabolite matrix with 65 identified metabolites, 39 of which were unique to this biological compartment. A number of these were related to gut microbial host co-metabolism. Faecal samples were the most metabolically variable between animals; acetate was responsible for the majority (28%) of this variation. Short chain fatty acids were the predominant features identified within this biofluid by 1H-NMR spectroscopy.
Conclusions: Metabonomics provides a platform for investigating complex and dynamic interactions between the host and its consortium of gut microbes and has the potential to uncover markers for health and disease in a variety of biofluids. Inherent variation in faecal extracts along with the relative abundance of microbial-mammalian metabolites in urine and invasive nature of plasma sampling, infers that urine is the most appropriate biofluid for the purposes of metabonomic analysis
Specialty farming in Idaho: Selecting a site
No full textBulletin no. 744 Moscow, Idaho :University of Idaho, College of Agriculture, Cooperative Extension System, 1992-10-01. Author(s): Barney, D.L.; Finnerty, T.L.; Mancuso, C.J
The Actors' Perceptions and Expectations of their Roles in BIM-based Collaboration
No full textThe inter-organisational collaboration with Building Information Modelling (BIM) is one of the hottest topics in construction sector nowadays. The implementation of BIM is a complex inter-organisational process, and the sharing of information among numerous actors from multi-disciplinary backgrounds may affect the actors’ role perception and performance. This study offers insights into the BIM roles of various actors by analysing a BIM-based project carried out by an integrated partnership across many tiers. The analysis identified inconsistencies between the actors' perceptions and their partners’ expectations of their BIM roles. Inconsistencies in BIM roles were more related to soft rather than hard (domain- or technical) skills. Mismatches were found in the architect's role, as it was deemed necessary to be more domain- and BIM-related, contrary to their perceptions. Likewise, the suppliers' role called for an enhanced BIM orientation. The paper concludes with set of suggestions for increasing the joint responsibility and supporting the multi-actor collaboration.Design & Construction ManagementSustainable Housing Transformatio
Strange Fellah! Review of Philip Butterss, An Unsentimental Bloke: The Life and Work of C.J. Dennis
No full textC.J. Dennis, author of The Songs of a Sentimental Bloke (1915), was once considered the unofficial poet laureate of Australia. Throughout his career he was erroneously conflated with his famous character 'the Bloke' in the public imagination. 'Many have imagined him as a sentimental bloke', argues Philip Butterss, 'an easygoing fellow with a naïve and slightly romantic view of the world'(1). Since three previous biographical works have the word 'sentimental' in the title, Butterss' decision to add the 'un' to 'sentimental' immediately signals his intention to examine the darker side of Dennis
) Corresponding author.
No full textwww.elsevier.comrlocatergeoderma 3D reconstruction and quantification of macropores using X-ray computed tomography and image analysis A. Pierret a,) , Y. Capowiez b, L. Belzunces b, C.J. Moran
The One, Two, Threes of Involuntary Bankruptcy: Creditor Numerosity in the Fifth Circuit
No full textIn The One, Two, Threes of Involuntary Bankruptcy: Creditor Numerosity in the Fifth Circuit, C.J. Baker examines how courts interpret and apply the creditor numerosity requirement under § 303(b) of the Bankruptcy Code in involuntary bankruptcy proceedings. The article focuses on the Fifth Circuit’s approach to determining how many qualified creditors must join an involuntary petition, particularly when disputes arise over counting eligible and disqualified creditors. Baker analyzes conflicting judicial interpretations and highlights the practical implications of these inconsistencies for creditors and debtors alike. The author critiques the Fifth Circuit’s reasoning in light of broader statutory and policy considerations, emphasizing the tension between procedural safeguards and creditor remedies. Ultimately, the article advocates for a more uniform and predictable standard to ensure fairness and efficiency in involuntary bankruptcy cases
An investigation of the oceanic skin temperature deviation
No full textSatellite and in-situ radiometric measurements of sea surface temperature (SST) together with conventional SST and meteorological parameters are used to provide a description of the ocean surface skin temperature deviation (skin temperature - bulk temperature, AT) for a transect made across the Atlantic ocean from 50°N 00°W to 23°S 35°W during September and October 1992. Methods of in-situ SST measurement are discussed and the errors associated with each technique are given. The principles of infra red radiometry are explained. The differences between the calibration strategies used to determine SST using infra-red radiometers from both in-situ and satellite platforms are reviewed and the errors associated with each technique are given. Differences between published in-situ infra red SST data indicate that there may be a bias in these data as a consequence of the calibration strategy adopted. The need for an inter calibration of in-situ infra red radiometer systems used for the validation of satellite SST is highlighted. Satellite SST algorithms are discussed and the principles of atmospheric correction are explained. The difference between the radiometric 'skin' temperature of the ocean and the conventional 'bulk' temperature at depth is defined. A review of current observations of AT is given. Several theoretical treatments of AT are reviewed. The definitions of the surface fluxes of heat and momentum are given. A description of the collection of data and an analysis of the calibration of the infra-red radiometer used to measure the skin temperature is presented.Data have been processed to obtain AT and the surface fluxes of heat and momentum have been evaluated according to the bulk aerodynamic formulae. The relationships between AT and the measurements made are presented for the entire data set and for day and night time observations separately. Four time series of observed data are presented and the local conditions during the time of measurement are used to discuss AT. AT has a mean value of 0.39°C ±0.3°C and is shown to be a persistent feature of the Atlantic ocean. Correlation analyses reveal the skin and bulk temperature fields to be correlated at length scales > 155 km. Night time correlations are consistently higher than the day time at all length scales. For this reason it is recommended that satellite validation data are only collected during the night. High sea states are shown to affect both in-situ and satellite observations of SST biasing these data warm. The regional nature of AT is presented which is related to the dominant atmosphere-ocean conditions for each region. AT is shown to be greatest at the higher latitudes and weak in the tropical regions.Several parameterisations of AT are used to obtain estimates of AT using the data collected. These are found to be inadequate to predict AT at small temporal scales. A regional dependence of AT is found in these parameterisations. The coefficient A, of the Saunders (1969) parameterisation has been evaluated and is shown to have a regional dependence on the local atmosphere ocean conditions. The coefficient Ci and Ci of the Hasse (1971) parameterisation have been evaluated using the data collected. These are Ci=4.74 and C2=1.22.A comparison between the Along Track Scanning Radiometer Average SST is presented. Satellite - in-situ bulk AT has been obtained and shown to be comparable to that observed in-situ. This comparison highlights the need to make skin SST validation measurements rather than bulk SST measurements. The ATSR ASST data are shown to return a SST accurate to better than 0.3°C
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