61 research outputs found
Development of a rapid method for qualitative detection of microbiological contamination based on enzymatically-induced ATP luminescence
No full textNamen je bil razvoj hitre metode za kvalitativno določanje mikrobiološke kontaminacije na osnovi encimsko sprožene luminiscence ATP. Nova metoda za določevanje mikrobiološke kontaminacije v sterilnih farmacevtskih izdelkih je zasnovana na uporabi instrumenta Celsis Accel, ki s pomočjo bioluminiscence dosega visoko stopnjo občutljivosti. To omogoča pridobitev rezultatov o sterilnosti izdelka v krajšem času kot standardna metoda testa sterilnosti. S pripravo redčitvenih vrst testnih mikroorganizmov smo ugotovili visoko občutljivost, tako na bakterijske kot tudi glivne celice. Z instrumentom lahko pri bakterijah zaznamo koncentracije 480±45 CFU/ml. Pri glivah pa lahko zaznamo koncentracije 35±18 CFU/ml. Sama metoda ne omogoča zaznavanja bakterijskih spor in bakterijskih endotoksinov, kar pa je prav tako pomembno za varen farmacevtski izdelek. Na rezultate analize imajo velik vpliv tekoča gojišča ki se redno uporabljajo za standardno metodo testa sterilnosti. Svetlobni signal tekočega gojišča lahko zamaskira svetlobni signal celice, s čimer lahko dobimo lažno negativni rezultat. Obravnavana metoda omogoča pridobitev rezultatov v 6-ih dneh, kar je za 8 dni hitrejše od standardnega testiranja sterilnosti. S tem je doseženo tudi hitrejše sproščanje sterilnih farmacevtski izdelkov na trg in posledično tudi hitrejša dobavo le teh do pacientov.The purpose was development of a qualitative rapid method for determining microbiological contamination in sterile pharmaceutical products. The method for determining microbiological contamination in sterile pharmaceutical products is based on the use of the instrument Celsis Accel, which achieves a high level of sensitivity through bioluminescence ATP. This enables obtaining results regarding product sterility in a shorter time compared to the standard sterility test method. By preparing dilution
series of test microorganisms, we have found high sensitivity to both bacterial and fungal cells. The instrument can detect bacterial concentrations at 480±45 CFU/ml and fungal concentrations at 35±18 CFU/ml. However, the method itself does not allow for the detection of bacterial spores nor bacterial endotoxins, which are important for a safe pharmaceutical product. The results of the analysis are influenced by the liquid growth medium used daily for the standard sterility test method. The light signal from the liquid medium can mask the light signal from the cells, resulting in a false negative result. The method in question, enables us to achive results in 6 days, which is 8 days faster than the standard method of sterility testing. This allows for a faster release of sterile pharmaceutical products to the market and thus faster delivery to patients
Proclamations
No full textTitle: Oglas (Announcement) and Proglas (Proclamation) Originally published: Zagreb, Ljudevit Gaj, 1834 and 1835 Language: Croatian The excerpts used are from Dubravko Jelčić, ed., Hrvatski književni romantizam (Zagreb: Školska knjiga, 2002), pp. 155–159. About the author Ljudevit Gaj [1809, Krapina (Northern Croatia) – 1872, Zagreb]: language reformer, journalist and printer. Descendant of a middle-class German-Slovak family which settled in Croatia in the eighteenth century, he finished sec..
Medieval pottery from the Borinci - Crkvište - Crni Gaj site discovered in 1961.
No full textU radu se objavljuju ulomci keramičkih posuda nađeni
1961. g. na lokalitetu Borinci-Crkvište-Crni gaj koji se nalaze
u Zbirci Odsjeka za arheologiju Filozofskoga fakulteta
u Zagrebu. Ulomci se uspoređuju s već objavljenim
cijelim posudama nađenima na istome lokalitetu. Pokušava
se odgovoriti na pitanje pripadaju li one naselju ili
groblju 11–12. stoljeća ili je pak riječ o više različitih pozicija
unutar lokaliteta koje ne pripadaju istom vremenu.
Autor smatra da je riječ o vremenski odvojenim grobljima
koja se nalaze na približno istoj poziciji. Najstariji nalazi
potječu iz 8. i 9. stoljeća.The fragments of ceramic vessels found in 1961 at the
Borinci-Crkvište-Crni gaj site held in the Collection of the
Department of Archaeology, Faculty of Humanities and
Social Sciences in Zagreb are published in this work. The
fragments are compared to already published whole vessels
found at the same site. An attempt is made to respond
to the question as to whether they belong to the settlement
or cemetery of the eleventh/twelfth century or if these account
for several different locales within the site that do not
date to the same time. The author believes that these are
chronologically separate cemeteries situated at roughly the
same position. The oldest finds date to the eighth and ninth
centuries
'Hvit Myra, Lysemask'
No full textDescriptions of white ants ('Pack-gaj', 'Hvit Myra', and with Chinese characters) and of a 'glow worm' ('Lysemask'), with the narrative ('Berättelse') of how the worm was captured on board a ship between Rio Gambia and Tenerife on 1 June 1756.
Drawings in Indian ink.
Author unknown, but possibly by some officer of the Swedish East India Company
Number Field Sieve: Pseudocodes and Software Implementation
No full textThe RSA cryptosystem has been the mainstay of modern cryptography since it was first introduced in 1978. RSA serves as the basis for securing modern e-commerce–it functions as the primary key exchange mechanism for the Secure Sockets Layer (SSL) protocol. It is used by US Government Personal Identity Verification (PIV) smart cards and the Department of Defense Common Access Card (CAC) for authenticating users, digitally signing and encrypting email. Due to the importance of this algorithm, cryptanalysts have been working for decades to identify weaknesses in the algorithm. Because the security of the RSA algorithm rests on the computational infeasibility of factoring large numbers, a good deal of research has been in the field of factorization. Of note was the introduction of the Number Field Sieve in 1993, which remains the fastest known algorithm for factoring large numbers. One of the most difficult aspects of the Number Field Sieve is the complexity of the algorithm, requiring a great deal of number theory simply to understand how the individual steps of the algorithm function. To this end, there are very few implementations of the algorithm that are coupled with concise and detailed descriptions of the algorithm. This thesis describes an implementation of the Number Field Sieve implemented using C++ in a straightforward manner–leaving efforts to improve this particular implementation as future work. Based on the implementation, the author was able to derive a set of pseudocodes that can be provided to students to gain a full understanding of the number field sieve algorithm. Finally, this thesis performs a number of experiments on this implementation–as well as other open source implementations that have been developed in the past few years. This thesis aims to identify the trade-offs within the algorithm that can be made based on the wide variety of parameters that can be applied. While some of these trade-offs are to be expected (e.g., the performance impact of using a lattice sieve over a line sieve), a more detailed understanding of the various options will aid both implementers and students in improving software implementations and–where possible–identifying methods for breaking the number field sieve algorithm into components and identifying which components are best implemented in hardware and which components are best implemented in software
Cicero – the teacher of the Silesian youth. Didacticism in rhetoric, that is application of the Ciceronian rhetoric on the example of the Silesian humanistic gymnasia from 16th to 18th centuries
No full textThe article contains a description of the Ciceronian rhetoric didactics in the Silesian humanistic
gymnasia from the 16th to the 18th century. In her article, the author intends to analyze how the
educators of the old Silesia cultivated the ancient Ciceronian tradition of eloquence. She observes
that Cicero’s authority was never questioned in Silesia despite the European echoes of the ‘dispute
about Ciceronianism’, which reached Silesia as well. This and other issues from the area of rhetoric
and didactics, on the example of Cicero's works and their reception in Silesia, are the concerns of
this paper.Cicero’s authority as an educator as well as the independence of rhetoric and didactics
were already respected by his contemporaries. The ancient Ciceronian eloquence
tradition was cultivated in Silesia with diligence. Cicero’s unanimous recognition as a
didactic authority in the writings of the former Silesian humanists appeased the so called
‘disputes over Ciceronianism’ in relation to the special role Cicero played as a literature
model and legislator, which were an important indicator of the change in literature and
culture. Preserved until these days in Catholic as well as Protestant schools, numerous
texts of Ciceronian imitations such as ‘performances-shows’ using imitatively and creatively
Cicero’s literary achievements are an evidence of the above. Showing an interest in practical, as well as didactic, application of rhetoric in real life, works of Bartłomiej
Keckermann popular in Silesia certainly have also influenced the special presence of
Cicero in the Silesian humanistic gymnasium.
Moreover, the Silesian handwritten rhetoric of Nepomucen Horth, written after 1740
(defined by the author as a date of ending the argument between the ‘Ciceronians’ and
‘Anti-Ciceronians’), indicates the constant interest in didacticism of the Ciceronian prose.
Meanwhile, at that time in Europe different, that is national patterns were more often
chosen. Cicero – the teacher has never stopped being present in Silesian schools. Also
nowadays, he is eagerly included in the Latin teaching and rhetoric syllabi as well as
chosen for translations by the students themselves. Is it because didacticism of the Ciceronian
prose has been for centuries perceived as subtle and balanced (as opposed to e.g.
Seneca’s didacticism), being an outcome of the masterly connected 3 basic rhetorical
principles docere, delectare, movere
Malakoff Towers in Lower Silesia – origins, architectural evolution, contemporary history
No full textWydaje się, że o wieżach nadszybowych typu malakow napisano już wiele, jednak – jak pokazuje autorka artykułu – wiele kwestii pozostaje nadal niejasnych, z dużą dozą swobody kwalifikuje się też poszczególne obiekty do tej grupy. W artykule autorka z jednej strony analizuje samo pojęcie, jego niejasności i nieścisłości dotyczące typowania samych obiektów górniczych, noszących miano malakowów, z drugiej – przygląda się dolnośląskim przykładom tych wież, analizując ich architekturę, nakreślając rozwój i przemiany oraz najważniejsze elementy składowe. Terminologię osadza przy tym w kontekście historycznym i administracyjnym, podkreślając same przemiany architektury budynków nadszybowych górnictwa kamiennego w 2. połowie XIX wieku, zestawiając ze sobą nadszybia basztowe – typu malakow i w formie budynków mieszkalnych.It would appear that much has already been written about Malakoff-type shaft towers, but, as the author of the article shows, there are still many issues that remain unclear, and there is considerable latitude in qualifying individual buildings to this group. In the article, the author, on the one hand, analyses the very concept itself, and the ambiguities and inaccuracies regarding the typification of the mining objects themselves which are called Malakoffs. On the other hand, she examines other examples of these towers in Lower Silesia, analysing their architecture, outlining their development and transformation, as well as the most important components. In doing so, she places the terminology in a historical and administrative context, highlighting the transformations to the architecture of hard coal mine shaft towers in the second half of the nineteenth century, comparing Malakoff-type towers with shaft towers in the shape of residential buildings
Metody oszacowania dokładności względnej danych ALS
No full textWobec ciągłego rozwoju lotniczego skaningu laserowego ALS (ang. Airborne Laser Scanning) koniczne stało się opracowanie efektywnej metody kontroli jakości danych pozyskanych w ten sposób. Autor zaprezentował trzy główne spotykane w literaturze podejścia do oszacowania względnej dokładności danych ALS: metodę porównania modeli DEM (ang. Digital Terrain Model), metodę porównania punktów i powierzchni TIN (ang. Triangulated Irregular Network) oraz metodę porównania elementów liniowych. Każda z metod bazuje na wyborze różnego typu obiektów kontrolnych, rozmieszczonych w pasach pokrycia między sąsiadującymi szeregami ALS. Dodatkowo przedstawione zostanie nowe podejście, opierające się na porównaniu kalenic i siatek wysokościowych, opracowane w Polsce w celu oceny wzglę dnej dokładności danych LiDAR (ang. Light Detection and Ranging) w ramach projektu ISOK (informatyczny system osłony kraju przed nadzwyczajnymi zagrożeniami).In view of the continuous development of ALS (Airborne Laser Scanning), it has become necessary to develop an effective method of controlling the quality of data obtained with the use of this technology. The author presents three main approaches used in literature for assessing the relative accuracy of the ALS data: the method of comparing DEM
models (Digital Terrain Model), the method of comparing points and TIN surfaces (Triangulated Irregular Network) and the method of comparing linear elements. Each of these methods is based on a selection of various types of control objects located in overlapping areas between strips of ALS. In addition, a new approach is presented, based on a comparison of roof ridge lines and elevation grids, developed in Poland in order to assess the relative accuracy of the LiDAR data (Light Detection and Ranging) within the ISOK project (IT System of the Country’s Protection against extreme hazards)
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