1,721,049 research outputs found
Lectin binding and identification of sialic acid acceptor sugars in rabbit oviduct under hormone administration
No full textConfocal laser microscopy to investigate myoepithelial cells in tissue blocks
No full textMyoepithelial cells (MEC) are “star-shaped” or “basket” cells that, in salivary glands and other exocrine glands, lie between the basal lamina and the secretory endpiece and ductal cells; MEC resemble smooth muscle cells and epithelial cells in several aspects. The occurrence and distribution of MEC vary among glands and species in adults and during growth and differentiation. The optical sectioning by confocal laser scanning microscopy allowed artifacts during observation to be minimal; in addition, this technical approach avoids continuously changing and adjusting the plane of focus either through the apex of epithelium or in order to cross-section the secretory and ductal elements. Moreover the CLSM evaluation of phalloidin-TRITC fluorescent patterns, here performed, enables visualization, in 3 dimentions, of the morphology of actin-containing cells and can be useful for obtaining more direct spatio-temporal information also on the cellular dynamics of salivary secretio
CHARACTERIZATION OF MOUSE SALIVARY-GLANDS BY WATER-CONTENT AND TYPE
No full textThe thermoanalytical analysis was applied to samples of sublingual, submandibular and parotid glands from sexually mature mice of both sexes. Findings indicated that the three salivary glands show a behaviour of water release characteristic for each type of gland. Derivative thermogravimetry curves concerned with the sublingual and parotid glands belonging to male and female subjects exhibited overlapped results. As regards submandibular gland, instead, some differences emerged between subjects of different sex. Water content and types in sublingual, submandibular and parotid glands were discussed and related to the different morphological expression, histochemical reactivity and chemical composition of these organ tissues
Modification of lectin binding in the mouse submandibular gland consequent to administration of secretagogues.
No full textEndogenous lectins immunodetected in the submandibular glands of mice treated with secretogogues.
No full textLectins as discriminant agents for differentiating glycoconjugates (Gcs) of lagomorph oviduct
No full textSialylation patterns of the mouse parotid secretory granules. Combined deacetylation, enzymatic degradation and lectin-gold binding
No full textWe investigated the lectin cytochemistry of control and sialidase-digested sections of the mouse parotid gland by postembedding techniques. PNA and DBA lectins were used and their affinity sites were localized by employing conjugates with horseradish peroxidase that then reacted with anti-horseradish peroxidase antibody and protein A-gold. Potassium hydroxide pretreatment also was used before sialidase/PNA and DBA binding to investigate sialic acid acetylation. Ultrathin sections were obtained from specimens embedded in the acrylic hydrophilic resin, Bioacryl. The acini of mouse parotid gland contained polymorphous secretory grannies differentially stained by the two lectins; the use of sialidase digestion and KOH deacetylation revealed that the sialic acids linked to beta-galactose are restricted to the electron-dense concentric areas of target granules, whereas the sialic acids linked to alpha-N-acetylgalactosamine contain C-4-acetylated groups and are preferentially located in rite electron-lucent regions of bizonal granule
The hare parotid gland: ultrastructure and histochemistry of acinar and ductal cells
No full textThe parotid gland of the hare Lepus europaeus was studied by electron microscopy. The parenchyma consists of secretory areas (acini) and ductal segments (intercalated, striated and excretory ducts). Acinar cells showed a marked heterogeneity due to the presence of secretory granules characterized by different size, electron-density and degree of membrane merging in addition to the occurrence of flattened and dilated rough endoplasmic reticulum. Acinar cells had variable affinity for PAS and PA-TCH-SP staining but were HID- and LIT-TCH-SP negative. Intercalated duct cells lacked secretory granules. Striated ducts were lined by four cell types namely light cells, dark cells, vacuolated cells, and typical striated cells. Excretory ducts showed some scalloping on the apical region of the light cells
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