3,550 research outputs found
Suppression of the Pth(Ts) phenotype mediated by the overproduction of tRNA maintains moderate levels of the Pth(Ts) protein
<p><b>Copyright information:</b></p><p>Taken from "Excess of charged tRNA maintains low levels of peptidyl-tRNA hydrolase in (Ts) mutants at a non-permissive temperature"</p><p>Nucleic Acids Research 2006;34(5):1564-1570.</p><p>Published online 15 Mar 2006</p><p>PMCID:PMC1408313.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Depicts the cellular growth of the (Ts) mutant strain AA7852 separately transformed with pVH124 (ΔU, ΔV), pVH125 (U, ΔV) or pVH119 (U, V) incubated at different temperatures. Isolated colonies of the independent transformants were streaked onto LB-Ap plates and incubated overnight at the indicated temperatures. () Presents the immunodetection of Pth(Ts) in the (Ts) mutant strain AA7852 separately transformed with pVH124, pVH125, pVH119, ptRNACCA (X, R, T, M) or pTH2 (W) and grown at 32°C prior to transfer at time = 0 min at 41 or 43°C. The concentration of Pth(Ts) protein was estimated by immunoblot analysis. The left lane shows purified wild-type Pth protein, which migrates slightly faster in SDS–PAGE than the Pth(Ts) variant (arrowed) ()
Emergence of oblique TS mode due to longitudinal wall oscillation in 2D channel flow
Stabilizing effects of wall oscillation on two dimensional (2D) Tollmein-Schlichting (TS) waves and oblique TS waves developing in 2D channel flow are numerically investigated using the Floquet method. It was shown from the previous study[1] that the wall oscillation mostly has stabilizing effect and then TS mode is occasionally more stable than the oblique mode. In the present study the characteristics of the stability are investigated in detail. Then it is cleared that the exchange of the most unstable mode form the 2D TS to the oblique TS can occur at a certain value of Reynolds number
A TS uszályhíd kifejlesztése és alkalmazása
The essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers.The essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers
The new CEN/TS 19100: Design of glass structures
Since the beginning of 2021, CEN/TS 19100 Design of Glass Structures has been available in its first three parts. The fourth part is expected soon. This Technical Specification of the European standards organisation CEN is as a pre-standard of a corresponding future Eurocode. These documents constitute the first ever comprehensive design code for the entire structural glass engineering field on the European market for the first time. In addition to a clear outline, the Technical Specification has been drafted to be compatible with EN 1990 “Basis of Design” and to address glass-specific design matters, particularly related to robustness and redundancy. Although the standard still has the status of a CEN/TS, thereby allowing the European nations the option of whether to introduce it, either in full or in parts, it already contains national openings through which the European countries can adapt the design results to their own safety level by National Determined Parameters (NDPs). Such an approach already anticipates the future Eurocode, which is expected to be published as EN 19100—Design of Glass Structures. This article provides some context on the history and concept behind the new documents and gives an overview of the design rules and the corresponding technical background of the different parts of CEN/TS 19100.Applied MechanicsArchitectural Technolog
Development of EMT/TS Co-simulation Using PowerFactory and PSS/E
As the scale and complexity of power systems increase, simulating them in efficient and accurate ways continues tobe a challenge in power systems engineering. Electromagnetic Transient (EMT) and Transient Stability (TS) simulation are the two main dynamic power system simulation methods. To simulate large and complex power systems in sufficient detail without sacrificing execution time, one of the idea is to perform a co-simulation that couples EMT and TS simulator. Although several attempts have been made to couple both simulator, only on rare occasions do these hybrid EMT-TS simulators couple two or more industry-adopted simulation tools.The objective of this thesis is to to develop and study the benefits and limitations of the Electromagnetic Transient – Transient Stability co-simulation based on PowerFactory and PSS/E, both of which are among the most extensively used simulation tools in industry and academia alike. With regards to the objective, the EMT-TS co-simulation using PowerFactory and PSS/E has been developed. Then, several tests are performed to evaluate the function of each composing part of the EMT-TS co-simulation, and to test the integration between all its component. Next, The developed co-simulation is applied to study cases and the results are compared to a monolithic EMT simulation to evaluate its accuracy and execution time. Furthermore, the effect of TS and EMT time step to the accuracy and execution time of EMT-TS co-simulation have also been investigated.The study case results show that the developed EMT-TS co-simulation has not been beneficial yet in terms of accuracy and execution time. Although the active power result shows a similar tendency with the monolithic EMT result, the difference between both are visible. The difference between both are more prominent in the reactive power result. The total execution time of the developed co-simulation in the study cases are in the range of 23-24 minutes, significantly larger than the total execution time obtained from the monolithic EMT simulation which is around of 12s. Also, it is found that reducing the TS time step from 0.02 s to 0.01 s slightly increases the total simulation time from 23 to 26 minutes. However, it does not contribute a significant improvement on the accuracy of the developed EMT-TS co-simulation. The result obtained from reducing the EMT time step to is the same with the result obtained using EMT time step. Moreover, the reduction of the EMT time step significantly increases the total simulation time from 23 to 42 minutes. The developed co-simulation still has a lot of room for improvement and further developments in this topic might increase its performance.Electrical Engineering | Sustainable Energy Technolog
TS-MPC for autonomous vehicles Including a TS-MHE-UIO estimator
© 2019 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.In this paper, a novel approach is presented to solve the trajectory tracking problem for autonomous vehicles. This approach is based on the use of a cascade control where the external loop solves the position control using a novel Takagi Sugeno-Model Predictive Control (TS-MPC) approach and the internal loop is in charge of the dynamic control of the vehicle using a Takagi Sugeno-Linear Quadratic Regulator technique designed via Linear Matrix Inequalities (TS-LMI-LQR). Both techniques use a TS representation of the kinematic and dynamic models of the vehicle. In addition, a novel Takagi-Sugeno estimator-Moving Horizon Estimator-Unknown Input Observer (TS-MHE-UIO) is presented. This method estimates the dynamic states of the vehicle optimally as well as the force of friction acting on the vehicle that is used to reduce the control efforts. The innovative contribution of the TS-MPC and TS-MHE-UIO techniques is that using the TS model formulation of the vehicle allows us to solve the nonlinear problem as if it were linear, reducing computation times by 10-20 times. To demonstrate the potential of the TS-MPC, we propose a comparison between three methods of solving the kinematic control problem: Using the nonlinear MPC formulation (NL-MPC) with compensated friction force, the TS-MPC approach with compensated friction force, and TS-MPC without compensated friction force.This work was supported by the Spanish Min-istry of Economy and Competitiveness (MINECO) and FEDER through theProjects SCAV (ref. DPI2017-88403-R) and HARCRICS (ref. DPI2014-58104-R). The corresponding author, Eugenio Alcalá, is supported under FI AGAURGrant (ref 2017 FI B00433).Peer ReviewedPostprint (author's final draft
PENERAPAN MODEL PEMBELAJARAN KOOPERATIF TIPE TWO STAY TWO STRAY (TS-TS) TERHADAP KEMAMPUAN KOMUNIKASI MATEMATIKA SISWA
The problem that the author encountered in class X SMK Palapa Bandar Lampung is the low ability of mathematical communication. The purpose of the study was to determine the difference in average mathematical communication skills of students who use the Two Stay Two Stray (TS-TS)cooperative learning model with TS-TS using the Conventional learning model in class X students even semester of SMK Palapa Bandar Lampung. This study used an experimental method, the population of the study was 123 students of SMK Palapa Bandar Lampung, samples taken using the Random Sampling Cluster technique, namely the X PM class as the Experiment class and the AP X Class as the Control class. Students\u27 mathematical communication skills are carried out with an essay test which amounts to 6 questions which are first tested for their validity and reliability. From the results of testing the hypothesis using the t-test obtained thit = 4.94 from the t distribution table at a significant level of 5% known tdaf = 1.67. So thit > tdaf so that it can be concluded that "There is an Effect of the Application of the Two Stay Two Stray Cooperative Learning Model (TS-TS) on the Mathematical Communication Ability of Class X Students in the even semester of SMK Palapa Bandar Lampun
Transition and wavy walls: an experimental study: An experimental study
A wide body of research exists which explores the effects of surface roughness or patterned wall shapes on instability growth and transition. Building on those works as well as recent experiments demonstrating passive laminar flow control using arrays of discrete roughness [3, 8], a set of spanwise-wavy walls is designed with the goal of suppressing instability growth in two-dimensional boundary layers. In a numerical investigation of Tollmien-Schlichting (TS) wave growth in the presence of streamwise boundary-layer streaks, Cossu and Brandt [1] found that stabilization of TS waves results from spanwise shear in the mean flow, which forms a negative contribution to production in the perturbation kinetic energy equation. Whereas previous efforts have employed streamwise vorticity developing in roughness wakes to provide the requisite mean-flow deformation, in this work stabilization is achieved through modulation of the no-slip surface. Miniature vortex generators (MVGs) have proven an effective means of producing streamwise streaks for transition delay [8], though relatively large streak amplitudes are necessary to counter their eventual decay through viscous dissipation. The notion motivating this work is that spanwise-wavy walls extended in the streamwise direction can produce a similar effect while avoiding bypass transition resulting from large-amplitude streamwise streaks. Toward that end, six wavy walls are used in a modular test model. When TS waves are excited upstream of the wavy walls, substantial delays in the onset of transition are observed for certain spanwise wavelengths compared with the flat-plate reference case
Opportunities for linking young surveyors across professional surveying member organisations and FIG
Warimiri Tavares, de Mello & Mendes
<i>Warimiri</i> Tavares, de Mello & Mendes g <i>en. nov.</i> <p>urn:lsid:zoobank.org:act: DE1766C9-E04C-452C-9EA3-116D6DC9215C</p> <p> <b>Type species.</b> <i>Warimiri madiba</i> <b>sp. nov.</b> (described below).</p> <p> <b>Etymology.</b> The name is an arbitrary combination of letters derived from two words of the Nheengatu: <i>wariní</i> = warrior + <i>mirĩ</i> = small. Nheengatu is a language created by the Jesuit priests to homogenize several native idioms of the Tupi branch, making it possible for them to communicate between the tribes. Nheengatu is considered the New Tupi language. The gender of the name is being established as neuter.</p> <p> <b>Diagnosis.</b> Fastigium of vertex blunt, moderately protruding, wider than scapus, not dentate below, and contiguous with the fastigium of frons (Figs. 2A–E, G–H; 6C–D; 7B–D; 11B–D; 14C–D, G–H; 19C–D). Dorsal surface of head, frons, and genae conspicuously rugose and punctuate (Figs. 2C–E; 7B–C; 11B–C; 14C–E); antennal scape with an inward blunt tooth (Figs. 2D; 11B; 14D), posterior portion of each antennomere darker than the remaining. Eyes subglobose, slightly broader than the fastigium of vertex (Figs. 2A–E, G–H; 6C–D; 7B–D; 11B–D; 14C–D, G–H; 19C–D). Pronotum slightly convex in lateral view; pronotal disk distinctly lighter than the lateral lobes, with rounded lateral keels and an almost inconspicuous medial keel, more notable on the posterior half, resembling a roof (Figs. 2B; H; 6B, D; 7C, E; 11C, E; 14B, H; 19B, D); anterior and posterior margins truncated, this last produced behind. Lateral lobes produced laterally, with cephalic margins straight and oblique, anteroventral angles obtuse, anteroventral margins sinuous and oblique, posteroventral angles obtuse, posterior margins sinuous, and humeral sinus inconspicuous (Figs. 2A, G; 6A, C; 7A, D; 11A, D; 14A, G; 16A, C). Prosternum smooth, meso- and metatasternum wider than long, trapezoid (Figs. 2F; 7F; 11F; 14F). Wings significantly reduced (micropterous), almost entirely covered by the pronotal disk posterior margin (Figs. 2G–H; 4A–B; 6A–D; 7A, C–E; 11A, C–E; 14G–H; 16A–B; 19A–D). In males, both tegmina slightly surpassing the first tergite anterior margin (Figs. 2G–H; 7A, C–E; 14G–H), and hind wings trifling (Figs. 2A–B; 14A–B). Left tegmen anal margin conspicuously prominent, very similar to the right tegmen protruding plectrum (Figs. 4A; 8A; 16A). Mirror of both tegmina membranous, hyaline, and subtriangular (Figs. 4A–B; 8A–B; 16A–B). Right tegmen plectrum flexible, with a hyaline and more membranous cell formed between two anal veins (Figs. 4B; 8B; 16B). In females, both pairs of wings even more reduced, trifling (Figs. 6A–D; 7A, E; 19A–D). Legs short and stout; anterior tibiae’s dorsal surface elevated into two low lateral keels (Figs. 3A–C; 7G; 11G; 15A–C); all femora dorsally unarmed (Figs. 3A–B, D–E, G–H; 7A, G–H; 11A, G–H; 15A–B, D–E, G–H); anterior femora with minute ventral spines internally (Figs. 3B; 7G; 11G; 15B) and externally generally smooth (Figs. 3A; 15A); mid femora usually smooth internally (Figs. 3E; 15E) and externally with minute ventral spines (Figs. 3D; 7H; 11H; 15D). Male subgenital plate widely expanded laterally and dorsally, emarginated posteriorly, with two cylindrical styli (Figs. 3J–L; 7I–K; 15J–L); cerci short, bearing inward spines distally or medio-proximally (Figs. 3M–N; 7I; 15M–N). Titillatory process (<b>ti</b>) comprising a continuous sclerotized area that can be limited to the dorsal fold (<b>df</b>) (Figs. 5A–B, E–F) or extends from the fold <b>df</b> to the dorsal cavity (<b>dc</b>) (Figs.17A–B, D–F). Titillator’s sclerites (<b>TS</b>) paired and long, rod-shaped (Figs. 1A–F; 5A–B, D–E; 9A, D–E; 17A–D); anterophallic membranous processes of the dorsal lobe (<b>mp.dl</b>) pared, attached to the apical most portion of sclerite <b>VS</b> (Figs. 5B–C, F; 9B; 17A–C, F); sclerite of the ventral fold of the dorsal lobe (<b>VS</b>) large, inverted Yshaped. Female subgenital plate narrow (basally narrower than the proximal portion of ovipositor ventral valves), emarginated posteriorly, not produced dorsolaterally, and basally flanked by two membranous invaginations (better seen when the subgenital plate is lowered) (Figs. 1H–I; 6G; 11K; 19G), located just before the anteriormost margins of the ventral valves (Figs. 1H–I, <i>white arrows</i>); ovipositor strongly upcurved (Figs. 6E; 11I; 19E).</p> <p> <i>Warimiri</i> <b>gen. nov.</b> is morphologically similar to <i>Hyperomerus</i> and <i>Dectinomima</i> and probably is closely related to them. However, <i>Hyperomerus</i> has two distinct sclerotized areas comprising the processes <b>ti</b>; processes <b>mp.dl</b> attached to the process <b>es ti</b> anterophallic surface; cerci usually elongated, bearing a compressed projection and a basal appendage or spine (only <i>Hyperomerus almeirina</i> Tavares, Sovano & Gutjahr has short cerci, with reduced compressed projection). On the other hand, <i>Dectinomima</i> has no processes <b>ti</b>; the sclerites <b>TS</b> are neither elongated nor rod-shaped, and the cerci bear an elongate mesointernal tapered projection anteriorly or posteriorly upcurved.</p> <p> <b>Comments.</b> Montealegre-Z <i>et al.</i> (2011) recorded two undescribed species tentatively determined as “near <i>Uchuca</i> ” (<i>i.e., Hyperomerus</i>) to the pacific coast of Colombia and Ecuador. Till the moment, it is not sure if these species belong to <i>Hyperomerus</i> or another undescribed genus. The available knowledge is that all species already described to <i>Hyperomerus</i> are limited to the East side of the Andes, extending to the Amazonian region (Tavares & Nunes-Gutjahr 2021), and the two species within <i>Dectinomima</i> are recorded to Panama and the Pacific coast of Colombia, on the trans-Andean region (Montealegre-Z & Morris 2003). <i>Warimiri</i> <b>gen. nov.</b> is the first genus of Agraeciini with blunt and large fastigium of vertex (larger than scapus) recorded in the Atlantic forest. Nowadays, this biome is isolated from the Amazon by two biomes of the ‘open formation diagonal’ (also known as ‘dry diagonal’): Cerrado and Caatinga (Batalha-Filho <i>et al.</i> 2013; Werneck 2011).</p> <p> The new species described here were collected in the States of Ceará, Alagoas (one species with disjoint distribution), and Bahia (two species). We believe that <i>Warimiri</i> <b>gen. nov.</b> is closer to <i>Hyperomerus</i> than <i>Dectinomima</i> due to the similarity of the phallic complex, but a phylogenetic approach is needed to confirm this hypothesis.</p> <p> <b>Comments on the phallic components.</b> Males genital structures tend to diverge faster than other morphological structures, driven by different select pressures (Simmons 2014). In katydids, the sclerites <b>TS</b> play an important role in female acceptance for copula by stimulating the internal membranes of the copulatory chamber (acting as a courtship device) (Wulff <i>et al.</i> 2015, 2018), applying pressure that forces the subgenital plate to stay open (Wulff <i>et al.</i> 2017) or mechanically supporting the mating position and the spermatophore transference (Wulff & Lehmann 2020). The copula duration varies among Tettigoniidae subfamilies, but it is significantly longer in species with sclerites <b>TS</b> than in species without these structures, and more complex sclerites <b>TS</b> transfer spermatophorous quicker (Vahed <i>et al.</i> 2011). In addition, many species of Tettigoniidae have processes <b>ti</b>, sclerotized areas that bear sclerotized microstructures, which probably also act as stimulation devices (Chamorro-Rengifo & Lopes-Andrade 2014), increasing the stimulatory capability of the phallus. However, morphofunctional studies have not considered these last structures (Vahed <i>et al.</i> 2011; Wulff <i>et al.</i> 2015, 2017, 2018; Wulff & Lehmann 2014, 2020). In fact, any other component of the phallic complex has not been considered, especially the internal ones.</p> <p> In <i>Warimiri</i> <b>gen. nov.</b> and <i>Hyperomerus</i>, the posterior portion of the phallic complex bears very long and paired sclerites <b>TS</b> and paired or single processes <b>ti</b>. Internally (or anteriorly), a very large sclerite <b>VS</b> and a pair of processes <b>mp.dl</b> are also present in both genera. Despite being on different faces of the phallus, in these two genera, the sclerite <b>VS</b> supports the sclerite <b>TS</b> eversion, acting as an umbrella’s runner (Figs. 1A–E). So when the phallus is everted (by the hydrostatic pressure of hemolymph), it is physically easier to push a unique structure (the runner) that, in turn, forces the two sclerites <b>TS</b> outward (acting as the umbrella’s stretchers) than directly forcing two independent mobile bars (Fig. 1G, <i>black arrow</i>). For us, this sizeable central sclerite also helps to maintain the phallus wholly everted.</p> <p> On the other hand, it is also easier to retract the phallus by pulling a unique central structure. However, we believe that a second component facilitates the phallus retraction, the processes <b>mp.dl</b>. These processes are located on the dorsalmost portion of the anterophallic face in both genera and are connected directly to the sclerite <b>VS</b> apex (in <i>Warimiri</i> <b>gen. nov.</b>) or the processes <b>ti</b> (in <i>Hyperomerus</i>). We believe these processes act like tendons, pulling back inside the sclerite <b>VS</b> (Fig. 1G, <i>white arrow</i>). Ventrally, a membranous projection of the phallus is connected to the subgenital plate and acts like a frenulum, helping to retract the phallus (Fig. 1F, <i>white arrow</i>).</p> <p> <i>Dectinomima</i> has shorter sclerites <b>TS</b> and apparently no processes <b>ti</b>, but we do not have any information about the anterophallic components of this genus, so we will not discuss it.</p>Published as part of <i>Tavares, Gustavo Costa, De Mello, Francisco De A. G. & Mendes, Diego Matheus De Mello, 2021, A new genus and three new species of Agraeciini (Orthoptera: Tettigoniidae: Conocephalinae) from the Brazilian Atlantic Forest, with comments on the function of some phallic components, pp. 201-227 in Zootaxa 5057 (2)</i> on pages 202-205, DOI: 10.11646/zootaxa.5057.2.3, <a href="http://zenodo.org/record/5588025">http://zenodo.org/record/5588025</a>
- …
