17 research outputs found
Evaluation of different saccharides and chitin as eco-friendly additive to improve the magnetic cross-linked enzyme aggregates (CLEAs) activities
No full textThe cross-linked enzyme aggregates (CLEAs) involves formation of a number of covalent bonds between enzyme and the matrix using glutaraldehyde. In general, amino groups of lysine, sulthydryl groups of cysteine, phenolic OH groups of tyrosine, or imidazol group of histidine are used for enzyme binding under mild conditions. The main advantage of this method is its simplicity, economic advantages in the industrial bio catalysis. The Fe3O4 magnetic nanoparticles were synthesized by coprecipitating Fe2+ and Fe3+ in alkaline solution. Tannic acid was used to functionalize the Fe3O4 magnetic nanoparticles. After functionalization process, tannic acid magnetic cross-linked enzyme aggregates of enzyme (TA-MNPs-CLEAs) were prepared by cross-linking of enzyme aggregates with different saccharides as additive. The present result reported high stability, simplicity, low cost and recyclability of a saccharide-TA-MNPs-CLEAs-enzyme make it efficient as a highly active biocatalyst in biotechnological applications. The obtained results suggest that disaccharides (maltose, sucrose and lactose) and polysaccharide such as starch are eco-friendly additives to TA-MNPs-lipase and TA-MNPs-CLEAs-peroxidase and can become a powerful biocatalyst in industry applications. (C) 2018 Elsevier B.V. All rights reserved
Characterization and immobilization of protease and lipase on chitin-starch material as a novel matrix
No full textProtease and lipase were purified from sunflower seeds by frequent purification steps with molecular weights of 72.90 kDa and 27.50 kDa, respectively. The purified lipase and protease were immobilized on various carriers by different methods of immobilization including physical adsorption, ionic binding and covalent binding. The enzymes prepared by covalent binding on a new support materials were made via the combination of chitin and starch had the highest activates. The immobilization was carried out in a simpler way compared with the other immobilization methods which require various chemicals and complicated procedures which is difficult and expensive. The influence of reusability, pH, thermal and storage stability of immobilizing enzymes compared to the free enzyme were studied. The immobilizing protease and lipase with chitin and chitin + starch were used to catalyze reactions through enhanced washing process. After adding immobilizing enzymes with chitin and chitin starch, the stain removal percentage of detergents was enhanced by 78% and 84%, respectively. We approve that these novel immobilizing protease and lipase with chitin + starch present a new approach to the synthesis of multi enzyme biocatalysts from cheap and friendly environmental sources. (C) 2018 Elsevier B.V. All rights reserved
Lipase-based on starch material as a development matrix with magnetite cross-linked enzyme aggregates and its application
No full textThe Fe3O4 magnetic nanoparticles were prepared by precipitating ferrous ion (Fe2+) and ferric ion (Fe3+) in alkaline solution. The Fe3O4 magnetic nanoparticles were modified by tannic acid. After functionalization process, two methods were used to immobilize Lipase on Fe3O4 magnetic nanoparticles. In the first method, novel tannic acid magnetic cross-linked enzyme aggregates of lipase (TA-MNPs-CLEAs) were synthesized by cross-linking of lipase aggregates and starch as co-feeder with Fe3O4 magnetic nanoparticles improved by tannic acid (TA-MNPs). In the second method, the lipase was successfully immobilized on the surface of TA-MNPs. The properties of Fe3O4 and its modified forms were examined by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), scanning electron microscopy (SEM) and zeta potential measurements. Novel TA-MNPs-lipase and TA-MNPs-CLEAs-starch-lipase were enhanced and provided an effective method to improve the activity and stability of lipase for biodiesel production. Using 1% TA-MNPs-lipase and TA-MNPs-CLEAs-starch (w/w of oil) conversions around 67.87, and 85.88%, respectively, were obtained at 40 degrees C after 2 h of reaction. Furthermore, the immobilized enzyme was easily recovered from the reaction mixture and reused. The obtained results suggest that TA-MNPs-lipase and TA-MNPs-CLEAs-starch-lipase can become a powerful biocatalyst for biodie-sel production. (C) 2018 Elsevier B.V. All rights reserved
The novel multi cross-linked enzyme aggregates of protease, lipase, and catalase production from the sunflower seeds, characterization and application
No full textThe cross-linked enzyme aggregates (CLEAs) have numerous economic advantages in the industrial bio catalysis. In the present study, the multi CLEAs containing protease, catalase, and lipase from the sunflower seeds using starch as a cofeeder as well as bovine serum albumin (BSA) are designed and prepared successfully. After optimization, multi CLEAs of enzyme have been prepared with ammonium sulfate (55% w/v), glutaraldehyde (100 mM), and 8 mg/mL of starch or 20 mg/mL of BSA. The activity recovery of protease, catalase, and lipase multi CLEAs-starch are 87, 61, and 60%, respectively. Whereas, CLEAs prepared with BSA are 74, 61, and 50% activity and multi CLEAs only 60, 44, and 41% of protease, catalase, and lipase, respectively. The multi CLEAs were used to catalyze the reactions for enhanced washing process. After adding multi CLEAs-starch, the stain removal percentage of detergents is enhanced by 83%.The present study reports a high stability, simplicity, low cost, and recyclability of the novel multi CLEAs from the sunflower seeds that make them efficient as a highly active biocatalysts in the biotechnological applications. We believe that these novel multi CLEAs present a new approach to the synthesis of multi enzyme biocatalysts from the cheap and friendly environmental sources
The effect of increased levels of lead in serum on several antioxidants parameters assed among workers from a large private electrical generator company
No full textLead poisoning occurs when lead builds up in the body, regularly over a period of months or years. Insignificant amounts of lead can cause serious health complications. The main objective of this study is to determine the effect of gases rising from electric generators and the lead-induced oxidative stress and adversarial health properties by biochemical markers in Iraqi workers employed on the operation of diesel generators mainly increased lead poisoning symptoms. Serum lead, advanced oxidation protein products (AOPP), total antioxidant activity (TAA), malondialdehyde (MDA), and other biochemical parameters were analysed in fifty workers in a large private electrical generator. The results of the study showed a significant elevation in lead, AOPP and MDA activities in Iraqi workers employed on the operation of diesel generators in association to healthy control samples. The results showed that elevated serum lead has a significant correlation with oxidative stress, and biochemical markers that may be help to detect impairment in the body function in lead exposed workers
Genetic polymorphisms of human transcription factor-7 like 2 (TCF7L2), beta-defensin (DEFB1) and CD14 genes in nephrolithiasis patients
No full textConclusions: The present findings are first of its class that validates a considerable connection of DEFB1 and TCF7L2 gene polymorphisms with nephrolithiasis and could probably act as indicators to estimate the risk associated to nephrolithiasis. (C) 2018 Elsevier B.V. All rights reserved
Levels of antioxidant enzymes and alkaline protease from pulp and peel of sunflower
No full textObjective: The activity of enzymes participating in the systems of antioxidant protection was assayed in the peel and pulp of sunflower. The essential roles of proteases in food stimulate research to find other sources of the enzyme especially from non-conventional sources. In the present work, we study several biochemical parameters in the pulp and peel of sunflower.
Methods: Pulp and peel of sunflower was extracted, antioxidant enzymes and non-enzymatic antioxidant were measured. Alkaline protease was measured and purified from pulp in sunflower.
Results: High carbohydrate concentration, beta-carotene, catalase and ascorbate peroxidase activities, free radical scavenging capacity and free flavonoid content were observed in the peel of sunflower. Whereas, MDA and ceruloplasmin activities were high in the pulp of sunflower.
Conclusions: The present study concluded that peel in sunflower are strong radical scavengers and can be considered as good sources of natural antioxidants for medicinal and commercial uses. Further analysis showed that protease activity was a significantly high in the pulp compared to the peel
Study the effect of increased levels of lead on sera alpha amylase activity, and some biochemical parameters from a large private electrical generators workers
No full textBackground Lead poisoning is a health condition that occur when people are showing
to lead compound through inhalation, swallowing, and rarely, through the skin. Lead
poisoning frequently occurs from repeated contact to small amounts of lead. Objective:
The aim of the current study is to evaluate the level of the alpha amylase activity , Lead
and several biochemical factors in sera workers which working to turn on and control
on the large private electrical generators. Results: The group50 male employed on large
private electrical generators workers, mean age (35.47 ± 5.29 years) comparing with 40
male (34.80±4.28) apparently healthy control , the result showed that serum of S. Lead
,α-amylase activity and specific activity, were significantly increase(p<0.001) in large
private electrical generators workers group compared to the control group.
Conclusion: Lead poisoning has serious risks to human health, and exposure to lead
possibly will decrease lifespan and have health effects in the long term for these
worker
Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian individuals
No full textObjective Nephrolithiasis is one of the most common disorders of the urinary tract. The aim of this
study was to examine a possible relationship between DNase I/II activity and E3 SUMO-protein ligase
NSE2 in the sera of nephrolithiasis patients to evaluate the possibility of a new biomarker for evaluating
kidney damage.
Methods Sixty nephrolithiasis patients and 50 control patients were enrolled in a case-control study.
Their blood urea, creatinine, protein levels and DNase I/II activity levels were measured by
spectrometry. Serum NSMCE2 levels were measured by ELISA. Blood was collected from patients of the
government health clinics in Kuantan-Pahang and fulfilled the inclusion criteria.
Results The result indicated that mean levels of sera NSMCE2 have a significantly increase (P<0.01) in
patients compared to control group. Compared with control subjects, activities and specific activities of
serum DNase I and II were significantly elevated in nephrolithiasis patients (P<0.01).
Conclusion This study suggests that an increase in serum concentrations of DNase I/II and E3
SUMO-protein ligase NSE2 level can be used as indicators for the diagnosis of kidney injury in patients
with nephrolithiasis
The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis
No full textBackground
The genetic features indicate a crucial role in nephrolithiasis. The present study was aimed to investigate the role of Glutathione-S-transferase Mu (GSTM1), Glutathione-S- transferase Theta (GSTT1) and endothelial nitric oxide synthase (eNOs) gene polymorphism in nephrolithiasis.
Methods
We involved a case-control study in which 480 individuals were divided into 240 healthy control and 240 patients with nephrolithiasis. For each patient and control, we measured biochemical criteria, levels of glutathione S-transferase, eNOs, GSTM1, GSTT1genes and eNOS genes polymorphism by PCR-RFLP.
Results
GSTM1 and GSTT1 null genotypes are not a risk features for nephrolithiasis. The eNOS frequency GG, GT, and TT genotypes by using Ban II enzyme as restriction enzyme were found to be (48.33, 36.67, and 15.00) %. The eNOS frequency TT, GT, and GG genotypes by using the Ban II enzyme as restriction enzyme were found to be 15.84, 25.83, and 58.33%, respectively. The result showed an increase in serum eNOs levels were in the patient's group comparing to control.
Conclusions
This work is the first in the literature to study the relation between eNOs genes polymorphisms and nephrolithiasis. The results conclude that TT genotypes in the eNOs genes are associated with an increase the oxidative stress in patients
