19 research outputs found
Involvement of MAPK/NF-κB signal transduction pathways: Camellia japonica mitigates inflammation and gastric ulcer
No full textPrebiotic competence of spirulina on the production performance of broiler chickens
No full textThe study was conducted to evaluate the prebiotic effects of Spirulina as a growth and immunity promoter for broiler chickens. Birds (n=100) were randomly and equally distributed into four groups (T0, T1, T2 and T3) and fed on a diet containing 0, 2, 4 and 8 g Spirulina/kg feed respectively for 4 weeks. The body weight was significantly (P<0.05) increased in the treatment groups fed with Spirulina diet from 7th days to 28th days old. FCR was also significantly (P<0.05) decreased among the treatment groups. Hematological parameters were significantly (P<0.05) increased except ESR which was decreased significantly (P<0.05) in the treatment group. Aspartate aminotransferase (ALT) and alanine aminotransferase (AST) level were significantly (P<0.05) decreased in all the treatment groups. The study suggests that, Spirulina is a good natural feed additive which has a tremendous effect to improve the broiler production and thereby may reduce the production cost. [J Adv Vet Anim Res 2015; 2(3.000): 304-309
Therapeutic competence of dried garlic powder (Allium sativum) on biochemical parameters in lead (Pb) exposed broiler chickens
No full textThe study was conducted to assess the therapeutic competence of garlic (Allium sativum) in lead (Pb) exposed chickens. The experimental birds (n=350) were grouped into T0 (as control), T1, T2, T3 and T4. The birds of group T1 was provided with lead acetate at 100 mg/kg body weight. Group T2 had lead acetate at 100 mg/kg b.wt. + 1% garlic supplement, whereas group T3 was fed with lead acetate at 100 mg/kg b.wt. + 2% garlic supplement, and group T4 had lead acetate at 100 mg/kg b.wt. + 4% garlic supplement. The mean values (mg/dL) of uric acid, blood urea nitrogen, creatinine, cholesterol, triglycerides, low density lipoprotein, high density lipoprotein, and blood glucose in the birds of group T1 were significantly increased (p<0.01) on day 42 of post-treatment. Elevation of these parameters was suggestive for the pathological involvement of different organs like liver, kidney, muscles. Statistical analysis of variance indicated that lead acetate at 100 mg/kg b.wt. + 2% garlic supplement (T3) resulted significant (p<0.01) ameliorative effect on the biochemical parameters as compared to the group T2 and T4. In conclusion, potency of garlic in reversion of the values of the biochemical properties in Pb exposed chickens was close to the normal levels of the values
Regulation of JAK2/STAT3 and NF-κB signal transduction pathways; Veronica polita alleviates dextran sulfate sodium-induced murine colitis
No full textSığırlarda fascioliasise karşı nitroksinil içeren iki farklı ticari ürünün etkinliğini karşılaştırılmalı olarak değerlendirilmesi
No full textAmaç: Bu araştırma nitroksinil içeren iki farklı ticari ürünün 18 sığırda karaciğer kelebeğine karşı karşılaştırılmalı etkinliğini belirlemek amacı ile yapıldı. Gereç ve Yöntem: Onsekiz sığır, her birinde 6 adet olan 3 gruba (A, B ve C) ayrıldı. Grup C hiçbir uygulama yapılmaksızın hastalı- ğın kontrol grubu olarak değerlendirilirken, grup A ve B’ye sırası ile ticari ürünler deri altı yolla uygulandı. Bulgular: A ve B gruplarında 3, 7, 14 ve 28. günlerde her gramdaki yumurta sayısında (EPG) önemli oranda (P<0.01) düşme görüldü. Kontrol grubunda 7. günde EPG değerinde önemli artış (P<0.01) belirlendi. Grup A ve B’de 3, 7, 14 ve 28. günlerde ortalama EPG düzeyi sırası ile %83.9-%80.8, %81.7-%80.7, %78.1- %77.0 ve %73.7-%61.2 olarak belirlenirken, kontrol grubunda sırası ile %6.7, %9.2, %21.4 ve %30.9 olarak belirlendi. İlaç uygulanan grup A ve B hayvanlarında canlı ağırlıkta artış (P<0.01) belirlenirken, kontrol grubunda değişiklik belirlenmedi. Öneri: Araştırma sonucu nitroksinil içeren iki farklı ticari ürü- nün sığırlarda fascioliasise karşı benzer etkinliğe sahip olduğu ifade edilebilir.Aim: This study was conducted to evaluate the comparative efficacies of two different commercial products containing nitroxynil against liver fluke infestation for a period of 28 days by using 18 cattle. Materials and Methods: Eighteen cattle were divided into 3 groups (A, B and C) where each group consisted of six cattle. Product A and Product B were injected subcutaneously to the cattle in group A and B, respectively. Cattle in group C were kept as infected control group without giving any treatment. Results: A significant (P<0.01) reduction of egg per gram (EPG) count was found on 3rd, 7th, 14th and 28th days of treated cattle of group A and B, respectively. The EPG count of control group were significantly (P<0.01) increased 7 day onwards up to experimental period. Reduction of mean EPG on 3rd, 7th, 14th and 28th day after treatment in group A and B were 83.9%-80.8%, 81.7%-80.7, 78.1%-77.0% and 73.7%-61.2%, respectively, whereas in control group the mean EPG were 6.7%, 9.2%, 21.4% and 30.9%, respectively. The body weight was increased significantly (P<0.01) after treatments in group A and B, respectively except untreated control group C. Conclusions: This result may indicate that two commercial products contain nitroxynil have similar efficacies against fascioliasis in cattle
Anti-Inflammatory and Gastroprotective Roles of Rabdosia inflexa through Downregulation of Pro-Inflammatory Cytokines and MAPK/NF-κB Signaling Pathways
No full textGlobally, gastric ulcer is a vital health hazard for a human. Rabdosia inflexa (RI) has been used in traditional medicine for inflammatory diseases. The present study aimed to investigate the protective effect and related molecular mechanism of RI using lipopolysaccharide (LPS)-induced inflammation in RAW 246.7 cells and HCl/EtOH-induced gastric ulcer in mice. We applied 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), nitric oxide (NO), reactive oxygen species (ROS), histopathology, malondialdehyde (MDA), quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry (IHC), and Western blot analyses to evaluate the protective role of RI. Study revealed that RI effectively attenuated LPS-promoted NO and ROS production in RAW 246.7 cells. In addition, RI mitigated gastric oxidative stress by inhibiting lipid peroxidation, elevating NO, and decreasing gastric inflammation. RI significantly halted elevated gene expression of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthetase (iNOS), and cyclooxygenase-2 (COX-2) in gastric tissue. Likewise, RI markedly attenuated the mitogen-activated protein kinases (MAPKs) phosphorylation, COX-2 expression, phosphorylation and degradation of inhibitor kappa B (IκBα) and activation of nuclear factor kappa B (NF-κB). Thus, experimental findings suggested that the anti-inflammatory and gastroprotective activities of RI might contribute to regulating pro-inflammatory cytokines and MAPK/NF-κB signaling pathways
IN VITRO ANTIBACTERIAL ACTIVITY OF MEDICINAL PLANTS TURMERIC, CHINNAMON, AND CLOVE AGAINST GM (+VE) AND GM (-VE) BACTERIA
No full textObjective: Various diseases are caused by different pathogenic microorganisms. Antibiotics are being used for treatment of these infectious diseases, yet unpredictable utilization of it leads towards antibacterial resistance. It is required to discover better approaches to battle against antibacterial resistance. Therefore, the study aimed to detect antibacterial sensitivity of ethanol extracts of Curcuma longa (turmeric), Cinnamomumzeylanicum (clove) and Syzygiumaromaticum (cinnamon) against Syaphylococcus aureus and E coli.
Methods: Prior to sensitivity testing, ethanol oils were extracted by an electric blender and each of the bacteria strains were cultured onto blood agar plate. Antibacterial activity was tested by agar well diffusion method where three different concentrations (50 μl, 75 μl and 100 μl) of selected plants extract were used so far as to measure the inhibition zone. Inhibition zone of the ethanol extract of these plants were calculated where three were found to be sensitive against Syaphylococcus aureus and E coli.
Results: Greater inhibition zone 14.5 mm, 18.25 mm, 21.5 mm at100 μl against Syaphylococcus aureus in case of cinnamon whereas the least inhibition zone was showed by turmeric and it was 9.00 mm, 11.00 mm, and 12.75 mm at 100 μl extract against E. coli.
Conclusion: Overall, all the ethanol extracts were found to effective against these two bacteria but cinnamon can be used as more effective antibacterial agent in both human and veterinary field after the toxicological test
Effects of garlic supplementation on parasitic infestation, live weight, and hematological parameters in Black Bengal goat
No full textThis study was conducted to determine the effects of garlic on egg per gram (EPG) count of feces for gastrointestinal parasites, live weight, and hematological parameters in Black Bengal goat. A total of 18 dry does of 18-22 months of age were divided into 3 groups as T0, T1 and T2; where, each group comprised of 6 goats. The goats of T0 were feed with normal feeds, whereas the goats of T1 and T2 were fed with normal feeds plus 25 mL and 50 mL of 10% water solution of garlic twice per day, respectively for 60 days. The EPG count was performed by McMaster counting chamber, and live weight was measured by digital electric balance. EPG count for gastrointestinal parasites was found significantly lower in the treatment groups as compared to T0. Weight gain was recorded significantly higher in the treatment groups. The hematological parameters like total leucocyte count (TLC), erythrocyte sedimentation rate (ESR), packed cell volume (PCV), hemoglobin (Hb) and total erythrocyte count (TEC) showed significant changes in the treatment groups. The study suggests that 10% water solution of garlic is a useful supplementation to decrease EPG count, body weight gain; thus, the 10% aqueous garlic solution can improve the general health condition of goat. [J Adv Vet Anim Res 2015; 2(3.000): 326-331
Green synthesis of fluorescent carbon dots from carrot juice for in vitro cellular imaging
No full text
