6 research outputs found
Fermentation optimization, purification and biochemical characterization of ι-carrageenase from marine bacterium Cellulophaga baltica
No full textOptimization of Fermentation Conditions for Carrageenase Production by <i>Cellulophaga</i> Species: A Comparative Study
No full textCarrageenases appear in various species of marine bacteria and are widely used for the degradation of carrageenans, the commercially significant sulphated polysaccharides. The carrageenase production ability of six different Cellulophaga species was identified, with ι-carrageenase being the most abundant carrageenolytic enzyme. C. algicola was the most potent strain, followed by C. fucicola and C. geojensis, whereas C. pacifica was the least effective carrageenase producer among the studied strains. The enzyme production was maximized using the one-factor-at-a-time optimization method. The optimal incubation temperature was identified as 25 °C and the incubation time was set as 48 h for all tested species. The optimal medium composition for Cellulophaga strains was determined as 30 g/L sea salt, 1.4 g/L furcellaran, and 3 g/L yeast extract. An ultrafiltered enzyme extracted from C. algicola had the highest activity at around 40 °C. The optimal pH for enzymatic degradation was determined as 7.8, and the enzyme was fairly stable at temperatures up to 40 °C
Determination of water quality and efficient removal of arsenic and iron from groundwater using mahogany fruit husk and banana peduncle charcoals
No full textThe groundwater (GW) of Bangladesh is predominantly contaminated with arsenic (As) and iron (Fe) which has a bad impact on human health. We tried to remove these elements with easily available mahogany-fruit (Swietenia mahagoni) husk charcoal (MHC) and banana (Musa acuminata) peduncle charcoal (BPC). The trial was implemented with 3 replications throughout the research. The sampled GW contained 0.06 mg As L−1 and 4.83 mg Fe L−1. Firstly, the pH was 3, 5, 7, and 9 with a 250 mg L−1 dose. The MHC removed almost 91.05 % of As at pH 5.0, and BPC removed almost 86.67 % of As at pH 9. However, in the case of Fe, the MHC removed almost 100 % at pH 7 and 9; and BPC removed the same quantity at pH 5, 7, and 9. Secondly, the contact times were 0, 5, 10, 20, and 40 min with a 250 mg L−1 dose at pH 7.0. The maximum removal of As and Fe was 100 % with MHC and BPC at 5 min. The pseudo-first-order kinetic, pseudo-second-order kinetic, and intra-particle diffusion models were considered. The result showed that the rate of adsorption followed the pseudo-second-order kinetic model. Lastly, the adsorbent doses were 0, 50, 150, 250, and 350 mg L−1. At pH 7, the highest removal of As was 79.47 % and Fe removal was 100 % at 350 mg L−1 dose for MHC. Similarly, the values were 79.29 % and 100 % for the same at 350 mg L−1 dose of BPC, indicating these charcoal are good for heavy metals removal
Biochemical Characteristics and Potential Biomedical Applications of Hydrolyzed Carrageenans
No full textSeaweed contains a variety of bioactive compounds; the most abundant of them are polysaccharides, which have significant biological and chemical importance. Although algal polysaccharides, especially the sulfated polysaccharides, have great potential in the pharmaceutical, medical and cosmeceutical sectors, the large molecular size often limits their industrial applications. The current study aims to determine the bioactivities of degraded red algal polysaccharides by several in vitro experiments. The molecular weight was determined by size-exclusion chromatography (SEC), and the structure was confirmed by FTIR and NMR. In comparison to the original furcellaran, the furcellaran with lower molecular weight had higher OH scavenging activities. The reduction in molecular weight of the sulfated polysaccharides resulted in a significant decrease in anticoagulant activities. Tyrosinase inhibition improved 2.5 times for hydrolyzed furcellaran. The alamarBlue assay was used to determine the effects of different Mw of furcellaran, κ-carrageenan and ι-carrageenan on the cell viability of RAW264.7, HDF and HaCaT cell lines. It was found that hydrolyzed κ-carrageenan and ι-carrageenan enhanced cell proliferation and improved wound healing, whereas hydrolyzed furcellaran did not affect cell proliferation in any of the cell lines. Nitric oxide (NO) production decreased sequentially as the Mw of the polysaccharides decreased, which indicates that hydrolyzed κ-Carrageenan, ι-carrageenan and furcellaran have the potential to treat inflammatory disease. These findings suggested that the bioactivities of polysaccharides were highly dependent on their Mw, and the hydrolyzed carrageenans could be used in new drug development as well as cosmeceutical applications
Removal of iron, manganese, and arsenic from groundwater by using banana and pineapple peel charcoal: evidence from sophisticated techniques
No full textThis study presents a novel and sustainable approach for removing iron (Fe), manganese (Mn), and arsenic (As) from groundwater using biochar adsorbents derived from banana (Musa ornata) peel (BP) and pineapple (Ananas comosus) peel (PP). Unlike conventional chemical or synthetic adsorbents, these agro-waste-derived materials provide a cost-effective and environmentally friendly alternative. The structural characterization of the developed biochars revealed porous surfaces with irregular cavities and well-developed microstructures as observed through Scanning Electron Microscopy (SEM), while Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of key functional groups (–OH, –COOH, and C=C) involved in metal binding. Groundwater analysis from Jaghati village, Jashore, Bangladesh, revealed elevated concentrations of Fe (4.14 mg L ¹), Mn (2.26 mg L⁻¹), and As (0.014 mg L⁻¹). Adsorption experiments demonstrated that BP charcoal achieved 96.31 % at 180 minutes with a 350 mg L-1 dose in pH 7.0, while PP charcoal achieved a maximum Fe removal efficiency of 98.97 % at 60 minutes with a 350 mg L-1 dose in pH 7.0. For Mn, BP charcoal showed a maximum removal of 83.12 % at pH 7.0 with a 250 mg L⁻¹ dose after 180 minutes. However, both adsorbents showed limited capacity for As removal, with maximum efficiencies of 11.67 % (BP charcoal) and 12.94 % (PP charcoal). The study highlights the promising potential of BP and PP charcoals for effectively removing Fe and Mn from groundwater, contributing to the development of low-cost, biodegradable treatment options for rural and resource-limited settings
Comparative analysis of proximate compositions, mineral and functional chemical groups of 15 different seaweed species
No full textSeaweed is a popular edible source and is associated with many foods and pharmaceutical industries around the world. The current research aims to provide information on the chemical composition of 15 seaweed species consisted of Chlorophyta, Ochrophyta/Phaeophyceae, and Rhodophyta macroalgae, collected from coastal areas of Sri Lanka. Seaweed samples were subjected to the analysis of lipids, proteins, ash and macro, micro, trace and ultra-trace elements. The highest protein content was recorded in the brown algae. Maximum dietary fiber and ash contents were recorded from green algae. The highest predominant fatty acids were observed from green seaweeds (Caulerpa racemosa); however, linoleic acid (C18:2n6) is the dominant fatty acid of all macroalgae. Mineral contents were highest in the red macroalga; however, copper, zinc and magnesium were also comparatively higher in green alga Ulva lactuca. In conclusion, 15 seaweed species belonging to the three different classes of seaweeds are investigated in detail to obtain their biochemical, mineral, and fatty acid compositions for the synthesis of novel therapeutic agents. In order to explore biorefinery processes for these seaweeds, as well as how they can potentially be cultivated, more extensive studies are required. Studying and determining the nutritional values of seaweeds will be beneficial with the potential for future industrial uses and research
