23 research outputs found
Rapid, Sensitive, and Sustainable Reversed-Phase HPTLC Method in Comparison to the Normal-Phase HPTLC for the Determination of Pterostilbene in Capsule Dosage Form
No full textThe greenness evaluation of literature analytical methods for pterostilbene (PT) analysis was not performed. Accordingly, the rapid, sensitive, and green/sustainable reversed-phase high-performance thin-layer chromatography (RP-HPTLC) method was developed and compared to the normal-phase (NP)-HPTLC (NP-HPTLC) for the estimation of PT with a classical univariate calibration. The RP quantification of PT was performed using green solvent systems; however, the NP analysis of PT was performed using routine solvent systems. The PT was detected at 302 nm for both of the methods. The greenness scores for the current analytical assays were evaluated by the analytical GREEnness (AGREE) metric approach. The classical univariate calibration for RP and NP methods indicated the linearity range as 10–1600 and 30–400 ng band−1, respectively. The RP method was more reliable for PT analysis compared to the NP method. The PT contents in commercial capsule dosage form were found to be 100.84% using the RP method; however, the PT contents in commercial capsule dosage form were determined as 92.59% using the NP method. The AGREE scores for RP and NP methods were 0.78 and 0.46, respectively. The sustainable RP-HPTLC assay was able to detect PT in the presence of its degradation products, and hence it can be considered as a selective and stability-indicating method. Accordingly, the RP-HPTLC method with univariate calibration has been considered as a superior method over the NP-HPTLC method for PT analysis
High-performance thin-layer chromatographic analysis of psoralen in marketed formulations and manufactured solid lipid nanoparticles (SLNs): Validation of the method
No full textStability-indicating high-performance thin-layer chromatographic method for analysis of terbinafine in pharmaceutical formulations
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Antiarthritic Potential of <i>Calotropis procera</i> Leaf Fractions in FCA-Induced Arthritic Rats: Involvement of Cellular Inflammatory Mediators and Other Biomarkers
No full textCalotropis procera (commonly known as Swallow wort) is described in the Ayurvedic literature for the treatment of inflammation and arthritic disorders. Therefore, in the present work, the antiarthritic activity of potential fractions of Swallow wort leaf was evaluated and compared with standards (indomethacin and ibuprofen). This study was designed in Wistar rats for the investigation of antiarthritic activity and acute toxicity of Swallow wort. Arthritis was induced in Wistar rats by injecting 0.1 mL of Freund’s complete adjuvant (FCA) on the 1st and 7th days subcutaneously into the subplantar region of the left hind paw. Evaluation of our experimental findings suggested that antiarthritic activity of methanol fraction of Swallow wort (MFCP) was greater than ethyl acetate fraction of Swallow wort (EAFCP), equal to standard ibuprofen, and slightly lower than standard indomethacin. MFCP significantly reduced paw edema on the 17th, 21st, 24th, and 28th days. It also showed significant effect (p < 0.01) on arthritic score, paw withdrawal latency, and body weight. The inhibition of serum lysosomal enzymes and proinflammatory cytokines along with improvement of radiographic features of hind legs was also recorded with MFCP. Finally, it was concluded that MFCP can be a feasible therapeutic candidate for the treatment of inflammatory arthritis
Review on cerebral malaria—Pathogenesis and role of EphA2 receptor in maintaining blood brain barrier integrity
No full textPlasmodium species causes cerebral malaria (CM), a dangerous parasitic illness. The World Health Organization estimates that there were roughly 228 million cases of malaria in 2018, which resulted in 405,000 tragic deaths. There was an approximate 20 % mortality rate among children diagnosed with CM who were admitted to the hospital. 67 % of the victims were children less than five years old. Patients who survive CM may experience lifetime post-CM complications and a higher risk of childhood neurodisability. The present review discuss about cellular mechanisms and immunological responses causing cerebral malaria. The breakdown of tight junctions and adherence junctions between endothelial cells (ECs) is the cause of an increase in the permeability of the blood brain barrier (BBB). It is obvious that this barrier can be breached by two different mechanisms: a) the apoptosis of ECs, and b) a loosening of the tight junctions between cells. T cells have been shown to have an important role in the development of cerebral malaria in an experimental mouse model (ECM). EphA2 receptors has been identified as contributing to many neurological illnesses and plays an important role in CM, associated with an impairment of the BBB. EphA2 is a key target protein that promotes EC apoptosis by targeting ephrin A ligand-expressing CD8+ T cell adhesion. Prominent role of the EphA2 receptors in CM has never been highlighted so far. Despite an intensive research, there is still a lack of successful malaria vaccines. The most advanced vaccines created so far, RTS,S/AS01 and R21/Matrix-M, focus on combating the early stages of the P. falciparum parasite. This review also discuss about the progress made for developing an effective vaccine for cerebral malaria
A STABILITY INDICATING HPTLC METHOD FOR THE ANALYSIS OF IRINOTECAN IN BULK DRUG AND MARKETED INJECTABLES
No full textChromatographic analysis of<i>trans</i>and<i>cis</i>-Citral in lemongrass oil and in a topical phytonanocosmeceutical formulation, and validation of the method
No full textInvestigating the Feasibility of Mefenamic Acid Nanosuspension for Pediatric Delivery: Preparation, Characterization, and Role of Excipients
No full textMolecules with poor aqueous solubility are difficult to formulate using conventional approaches and are associated with many formulation delivery issues. To overcome these obstacles, nanosuspension technology can be one of the promising approaches. Hence, in this study, the feasibility of mefenamic acid (MA) oral nanosuspension was investigated for pediatric delivery by studying the role of excipients and optimizing the techniques. Nanosuspensions of MA were prepared by adopting an antisolvent precipitation method, followed by ultrasonication with varying concentrations of polymers, surfactants, and microfluidics. The prepared nanosuspensions were evaluated for particle size, morphology, and rheological measures. Hydroxypropyl methylcellulose (HPMC) with varying concentrations and different stabilizers including Tween® 80 and sodium dodecyl sulfate (SLS) were used to restrain the particle size growth of the developed nanosuspension. The optimized nanosuspension formula was stable for more than 3 weeks and showed a reduced particle size of 510 nm with a polydispersity index of 0.329. It was observed that the type and ratio of polymer stabilizers were responsive on the particle contour and dimension and stability. We have developed a biologically compatible oral nanoformulation for a first-in-class drug beautifully designed for pediatric delivery that will be progressed toward further in vivo enabling studies. Finally, the nanosuspension could be considered a promising carrier for pediatric delivery of MA through the oral route with enhanced biological impact
