619 research outputs found
Il 20. siècle di Gualtieri di San Lazzaro
No full textCatalogo della mostra a cura di Luca Pietro Nicoletti e Luigi Sansone, tenutasi a Milano, presso la Biblioteca Sormani, dal 12 gennaio all'11 febbraio 2012, in occasione della ripubblicazione di:
Gualtieri di San Lazzaro, Parigi era viva, a cura di Luca Pietro Nicoletti, Firenze, Mauro Pagliai editore, 2011
DNA fusion product of phage P2 with plasmid pBR322: a new phasmid.
No full textThe chromosome of the temperate bacterio phage P2 and that of the plasmid pBR322 have been joined in vitro after treatment with restriction end on uclease EcoRl. The fusion product -a phasmid -can behave as a plasmid, as a phage and as a prophage. It can replicate its DNA under the control of either the specific replication mechanism of the parent phage in a polA mutant or that of the parent plasmid in a rep mutant. Several interesting interactions between the two replication modes are indicated. In particular, phage particles may be produced even when the phage mode of DNA replication is blocked, and this throws new light on the involvement of the early gene A in the regulation of late gene expression in phage P2
Professor Philippe Rasoanaivo Unveiling the ethnopharmacological potential of the flora of Madagascar: in memory of Philippe Rasoanaivo
No full textProfessor Philippe Rasoanaivo, University of Antananarivo, Madagascar, died on Wednesday,
13 July 2016 from a heart attack, at the age of 70. Professor Rasoanaivo was well known as one of the most distinguished African phytochemists, as also recognised by several international prizes. He won the 2015 Olusegun Obasanjo Prize for using traditional medicine to improve the efficacy of existing drugs for brain disorders and also treating sexual dysfunction among men. He had just been awarded the Prize at the last General Assembly meeting in Kasane, Botswana, in June 2016. Professor Rasoanaivo authored several
books and more than 150 international research articles, including some recently published in Natural Product Research. Professor Rasoanaivo developed a unique mixture of scientific knowledge dedicated to the utilisation of endemic plants for ethnobotanical, economic, medicinal and social uses. He wanted to learn everything that was necessary to understand and study the extraordinary flora of his country, obtaining this information to help its people. Throughout his life, countless examples of his remarkableness can easily be found. He was an excellent botanist and, living in a country well known for an exceptional endemic flora, he was able to find and collect many species, and show the world their importance. He was a great ethnopharmacologist, learning the popular uses of plants from the people of his country, and using this information to start a scientific validation of their efficacy. He was a modern researcher: in times of fragmented research, he was able to perform the full range of research on natural products, from chemistry to applied pharmacology. He was successful and organised director of the Institut Malgache de Recherches Appliquées, promoting the cultivation of crops of economic importance. In this obituary, we have provided a synthesis of his important scientific achievements and human qualities, since we had the privilege of working with him and he was able to teach us what modern ethnopharmacology is
Thucydides and the Politics of Plague
No full texthe paper analyses Thucydides’ description of the plague which ravaged Athens for two years, in 430–429 bc. In the first part of the paper, a detailed comparison with the medical, Hippocratic, corpus is provided, proving that the historian was familiar with that tradition and its methods. The second part of the paper, however, argues that the relationship with the medical tradition does not play a prominent role in the Thucydidean account. What matters most for him is the exploration of the social consequences of the pandemic. The plague uncovers a more violent and greedy dimension, an unsocial and anti-political aspect of human nature which remains hidden in peaceful times. By insisting on this point, Thucydides can thus oppose the common belief that human society had progressed from an unsecure and violent past which was dominant in fifth-century Athens
Turnover of carbamylphosphate synthase, of other mitochondrial enzymes and of rat tissues.
No full textThe relative rate constant of protein degradation for a number of enzymes as well as for rat liver
fractions and for ‘soluble’ and ‘insoluble’ protein of rat tissues has been measured by the
double-isotope technique. This technique has been evaluated and found reliable for measurements
involving half-lives of about 2 - 8 days and therefore generally useful for liver, kidney and possibly
heart but not for brain and skeletal muscle.
Carbamyl-phosphate synthase represents the major component (z 20 %) of the protein of rat
liver mitoplasts. Particular attention was given to determine its turnover, for obviously this enzyme
should be a good ‘marker’ for comparative studies with other mitochondrial proteins. The also
hitherto unknown half-lives of malate dehydrogenase and of glutamate dehydrogenase from rat
liver mitochondria have been measured.
The rate constants of degradation for rats fed ‘basal’ diet were: ‘homogenate’, kd = 0.20 day-’ ;
‘mitochondria’, k d = 0.10 day-’; ‘microsomes’, k d = 0.28 day-’; ‘cytosol’, k d = 0.20 day-’ ;
malate dehydrogenase, k d = 0.27 day-’ ; carbamyl-phosphate synthase, k d = 0.09 day-’ ; heart
proteins: ‘insoluble’, k d = 0.086 day-’ ; ‘soluble’, kd = 0.175 day-’; kidney proteins: ‘insoluble’,
k d = 0.28 day-’ ; ‘soluble’, k d = 0.245 day-’. Contrary to reports showing faster degradation of
long polypeptide chains than those of smaller size, the apparent turnover rate of carbamyl-phosphate
synthase is slower than those of other mitochondrial enzymes with smaller subunits. Similar
measurements were carried out with tissues from rats fed ‘high-protein’ and ‘protein-free’ diets and
after thyroidectomy. Rats fed a high-protein diet showed a general increase of the rate constant
of protein degradation in all the liver fractions studied when compared with data from animals
on the basal diet. Feeding a protein-free diet produced an increase of the rate constant of
degradation of liver mitochondria and fractions thereof. Thyroidectomy decreased the rate constant
of degradation in all the rat liver fractions and enzymes thereof. Kidney and heart insoluble and
soluble proteins behaved in the same way as those from rat liver. This is the first time that a direct
correlation of the often-mentioned, but hitherto not directly measured, effect on protein turnover has
been determined.
Small-scale purification of carbamyl-phosphate synthase, malate dehydrogenase and glutamate
dehydrogenase from a single rat liver is described. The preparations showed a high degree of
homogeneity by disc gel electrophoresis and by sodium dodecyl sulfate electrophoresis. Small-scale
purification, when applicable, should minimize uncertainties in regard to homogeneity of enzymes
used for antibody production in protein turnover studies. Moreover, it is more economical in time
Two linalool-1-oic acids from Kickxia spuria.
No full textThe ethanolic extract of K.spuria contains, besides the iridoid glucoside kickxioside, two linaloo-1-oic acids, whose structure were assigned by analysis of spectral data
Protezione sismica con dispositivi basati sulle leghe a memoria di forma: il progetto MANSIDE e gli ulteriori sviluppi
No full text- Productions of ochratoxin A by a strain of Aspergillus ochraceus on different seeds
No full textThe production of OTA A was studied using selected seeds as sterile food substrates for A. ochraceus
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