2,377 research outputs found
Inter- and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex
No full textThe data are whole-cell patch-clamp recordings from neurons in the medial entorhinal cortex of adult mice. The data for each neuron is a series of voltage responses to current steps or ramps injected through the recording electrode.There are two .tar.gz archive files. One contains stellate cell data and the other contains calbindin cell data. Each file has a similar folder structure when unzipped. The original data was recorded with Axograph and is in the folder 'multi_data'. The folder structure is '/multidata/slice/cell', where slice and cell are names specific to each experiment / recording. The electrophysiology data here is in .axgd format. To open .axgd files you can either use Axograph (available commerically from https://axograph.com/) or Neuromatic (http://www.neuromatic.thinkrandom.com/) which requires IGORpro (available commercially from (https://www.wavemetrics.com/). It should
also be possible to open the data using the axographio Python package
(https://pypi.org/project/axographio/) but we haven’t tested this. Most 'cell' level folders also contain an image of the slice in .jpg format. The electrophysiology data is also provided in hdf5 format in the folder name 'Database'. These files end in '.h5'. The folders also contain code to build the .h5 files from the .axgd files and then to automatically extract the parameters used for further analyses. This code should be sufficient to replicate the data processing. We plan to separately document and make this code available via our Github site (https://github.com/MattNolanLab)
Episode 39: Gophers softball on a roll in final stretch of regular season
No full textRuntime 17:17In this week’s episode of the “Weekly Rundown,” our staff discusses the Gophers softball team’s recent success, the team’s series against Nebraska and what to expect in the waning stretch of the season.O'Hara, Nolan; Kennedy, Matthew. (2021). Episode 39: Gophers softball on a roll in final stretch of regular season. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/258193
'Woe to you, hypocrites!' : law and leaders in The Gospel of Matthew
No full textThis thesis seeks to move beyond the impasse in Matthean scholarship that posits the reason for conflict in Matthew 23 with the authorial community. A framework is developed that allows the possibility that the gospel was received and understood by a widespread, general audience that itself was not necessarily embroiled in conflict. Multiple complementary methods are used to analyze how an ancient audience might expect conflict and work through its development in the narrative. Analysis of comparative biographical literature and of Old Testament references and allusions shows that readers could expect in literature the type and intensity of conflict exhibited in Matthew 23. The gospel's internal narrative development provides unity to the conflict episodes in Matthew 9-23. It also offers rationale for the escalation of conflict for which Matthew 23 is the summary. Chapter One: The Shape of the Discussion surveys representative works including redaction, social scientific, socio-historical, narrative and genre critics, to understand the options for studying conflict in Matthew. Reader-response oriented genre criticism provides language for framing reader expectations. Chapter Two: Expecting Conflict examines expectations that can be associated with Matthew's use of the Old Testament and by comparison with ancient biographies. Chapter Three: The Conflict Builds works systematically through each of the points of contact between Jesus and the leaders of Israel in chapters 9-22 organized by three topics: legal interpretation, the identity and authority of Jesus, and the character of the leaders. Chapter Four: Woe to You takes up the task of examining Matthew 23. The analysis of Matthew 23 identifies three components in the summary of conflict: Jesus presented as the model for his audience, Jesus' final denunciation of the leaders, and the presentation of Jesus as God’s representative. The multi-methodological approach used in this study of Matthew 23 suggests a narrative that invites the reader to rethink how one knows and understands God. The study thereby provides an alternative to the assumption that conflict reflects the immediate experience of a narrowly conceived authorial community
Zechariah and the Gospel off Matthew: the use of a biblical tradition
No full textThis thesis examines the use of Zechariah traditions in Matthew's Gospel. It analyzes and interprets the ways Matthew transmits, alters or adds Zechariah traditions to his sources. Instead of looking at portions of the Gospel in light of Zechariah 9-14 only, this study addresses the entire Gospel and all of Zechariah. In focusing on Zechariah tradition, the thesis has kept the following considerations in view. First, the content and function of Matthew's explicit uses of Zechariah are examined. Second, ways in which tradition derived from Zechariah may have exerted influence on portions of the gospel sub-structure are identified. Third, it explores the extent to which Matthew alludes to characteristic Zechariah themes. Together, these components illuminate how Matthew's Gospel incorporates its Zechariah material, whether alone or in combination with other prophetic traditions. Thus the methodological approach of the thesis is not only grounded in classical methods of biblical criticism but is also open to recent literary methods. In addition to explicit citations, numerous allusions and echoes of Zechariah tradition are present in Matthew. They appear in Matthean materials and in traditions Matthew has taken from Mark and Q. Because the focus of this thesis is open to both the Gospel and the Zechariah traditions in their entirety, two important observations have been made. First, traces of Zechariah material are found in the Infancy and Gaililean healing Narratives as well as in the Passion Narrative. Not only is the impact of Zechariah 9-14 observed, but important sections of Zechariah 1-8 are also discerned in Matthew's narrative structure. Moreover, Matthew's Son of David Christology is enriched and partially defined by Zechariah's prophet-shepherd imagery, as well as by the royal messianic motif
Active integration of glutamatergic input to the inferior olive generates bi-directional postsynaptic potentials
No full textThe inferior olive plays a critical role in motor coordination and learning by integrating diverse afferent signals to generate climbing fibre inputs to the cerebellar cortex. While it is well established that climbing fibre signals are important for motor coordination, the mechanisms by which neurones in the inferior olive integrate synaptic inputs and the roles of particular ion channels are unclear. Here, we test the hypothesis that neurones in the inferior olive actively integrate glutamatergic synaptic inputs. We demonstrate that optogenetically activated long-range synaptic inputs to the inferior olive, including projections from the motor cortex, generate rapid excitatory potentials followed by slower inhibitory potentials. Synaptic projections from the motor cortex preferentially target the principal olivary nucleus. We show that inhibitory and excitatory components of the bidirectional synaptic potentials are dependent upon GluA receptors, are GABAA -independent, and originate from the same presynaptic axons. Consistent with models that predict active integration of synaptic inputs by inferior olive neurones, we find that the inhibitory component is reduced by blocking large conductance calcium-activated potassium channels with iberiotoxin, and is abolished by blocking small conductance calcium-activated potassium channels with apamin. Summation of excitatory components of synaptic responses to inputs at intervals ≤ 20 ms is increased by apamin, suggesting a role for the inhibitory component of glutamatergic responses in temporal integration. Our results indicate that neurones in the inferior olive implement novel rules for synaptic integration and suggest new principles for the contribution of inferior olive neurones to coordinated motor behaviours
Phthinomita hallae Nolan & Cribb 2006, n. sp.
No full textPhthinomita hallae n. sp. (Figs. 29–31) Type host: Siganus doliatus (Cuvier), Barred Rabbitfish (Perciformes: Siganidae). Other hosts: S. corallinus (Valenciennes), Coral Rabbitfish; S. vulpinus (Schlegel & Müller), Foxface. (Perciformes: Siganidae). Site in host: Intertrabecular spaces of ventricle (heart). Type locality: Heron Island, southern Great Barrier Reef (23°27'S 151°55'E), Queensland. Material examined: ex S. doliatus, Heron Island (QLD), Apr. 2001, Jul. 2001, Feb. 2002, twenty specimens (Holotype no. QM G 225563; Paratype nos. QM G 225564–225572); ex S. corallinus, Heron Island (QLD), May. 2003 (one specimen sequenced for ITS2); ex S. vulpinus, Heron Island (QLD), Feb. 2002 (two specimens sequenced for ITS2). Collector: M.J. Nolan. Etymology Specific name for our good friend and colleague Dr Kathryn Hall, for her efforts in teaching MJN phylogenetic theory and her continued friendship and advice over the last six years. Description Based on 20 partial and complete mounts. With features of genus. Body slightly notched level with male genital pore. Anterior testis originating posterior to posterior caeca, margins lobed. Posterior testis ovoid, margins lobed. Cirrussac spherical. Internal seminal vesicle spherical, occupying most of cirrussac; ejaculatory duct straight; prostatic cells not seen. Ovary spherical, slightly overlapping posterior margin of anterior testis sinistrally. Oviduct originating at centre of posterior margin of ovary, sinuous, dorsal to vas deferens, entering oötype posterodorsally. Vitelline duct forming posterior to posterior margin of ovary, passing posteriorly dextral to vas deferens; vitelline reservoir forming posterior to cirrussac, sinuous, entering oötype posteriorly. Oötype ovoid. Mehlis' gland extending anteriorly past anterior margin of oötype, extending posteriorly to anterior margin of posterior testis. Uterus extending from oötype, sinuous, sinistral to oviduct. Uterine chamber forming posterior to posterior margin of ovary, sinuous, curving dorsally posteriorly to female pore. Vitelline follicles extending anteriorly past intestinal bifurcation, extending posteriorly to anterior margin of uterine chamber. Remarks Phthinomita hallae differs from other congeneric species in possessing the combination of a body 3070–3950 (3507) long, an anterior testis that originates posterior to the intercaecal field and that occupies 35–37% of the body length, an anterior testis 7.6–9.9 times longer than the posterior one, only a slight notch level with the male genital pore (by comparison to that of P. brooksi), a spherical cirrussac, a uterine chamber situated entirely posterior to the ovary and that is 104–150 (126) x 10–19 (14) and vitelline follicles that extend anteriorly just past the intestinal bifurcation. There are in addition, 3–35 base differences (0.8–10.7% sequence divergence) between the ITS2 rDNA sequence of P. hallae and the remaining Phthinomita species sequenced here. Between the sequences from P. hallae (21 replicates) from three sympatric hosts off Heron Island and P. jonesi (25 replicates) from four sympatric hosts off Lizard Island there are three base differences.Published as part of Nolan, Matthew J. & Cribb, Thomas H., 2006, An exceptionally rich complex of Sanguinicolidae von Graff, 1907 (Platyhelminthes: Trematoda) from Siganidae, Labridae and Mullidae (Teleostei: Perciformes) from the Indo-west Pacific Region, pp. 1-80 in Zootaxa 1218 (1) on pages 43-45, DOI: 10.11646/zootaxa.1218.1.1, http://zenodo.org/record/506485
Phthinomita symplocos Nolan & Cribb 2006, n. sp.
No full text<i>Phthinomita symplocos</i> n. sp. (Figs. 8, 15–22) <p> Type host: <i>Siganus lineatus</i> (Valenciennes), Goldlined Rabbitfish (Perciformes: Siganidae).</p> <p>Site in host: Intertrabecular spaces of ventricle (heart).</p> <p>Type locality: Lizard Island, northern Great Barrier Reef (14°40’S 145°27’E), Queensland.</p> <p> Material examined: ex <i>S</i>. <i>lineatus</i>, Lizard Island (QLD), May 2004, twenty­seven specimens (Holotype no. QM G 225528; Paratype nos. QM G 225529–225537) (another three specimens used for scanning electron microscopy and nineteen specimens sequenced for ITS2).</p> <p>Collector: M.J. Nolan.</p> <p> <i>Etymology</i></p> <p> Specific name “ <i>Symplokos</i> ” (Gr. interwoven, involved), for the host­parasite system described here.</p> <p> <i>Description</i></p> <p>Based on 27 whole mounts. With features of genus. Body slightly notched at male genital pore. Vestigial oral sucker bearing 5 concentric rows of fine spines. Intestine; posterior caeca irregular, distal portions expanded. Anterior testis originating posterior to intercaecal field, margins lobed, containing dorso­ventral muscle fibres. Posterior testis elliptical, margins lobed, rudimentary (may have sperm anteriorly, testis tissue posteriorly (see Figure 8a)). Vas deferens seen antero­dextral to posterior margin of anterior testis; duct from posterior testis passing antero­medially. Cirrus­sac tear­shaped. Internal seminal vesicle ovoid, occupying posterior of cirrus­sac; ejaculatory duct sinuous; prostatic cells not seen. Ovary spherical to ovoid, slightly overlapping posterior margin of anterior testis. Oviduct originating at posterior dorsal margin of ovary, dorsal to vas deferens, entering oötype postero­dorsally. Vitelline duct forming anterior to cirrus­sac, posteriorly dextral to vas deferens and cirrus­sac. Oötype ovoid. Mehlis’ gland extending anteriorly to posterior margin of cirrus­sac, extending posteriorly to anterior margin of posterior testis. Uterus extending from oötype sinuously, sinistral to oviduct. Uterine chamber forming posterior to posterior margin of ovary, sinuous, curving dorsally posteriorly to female pore. Vitelline follicles extending anteriorly past intestinal bifurcation, sinistral and dextral to oesophagus, posterior caeca and anterior testis, posteriorly extending to mid­section of uterine chamber.</p> <p> <i>Remarks</i></p> <p> There are 7–35 base differences (1.9–10.7% sequence divergence) between the ITS2 rDNA sequence of <i>P. symplocos</i> <b>n. sp.</b> and the remaining <i>Phthinomita</i> species sequenced here. Between the sequences from <i>P</i>. <i>symplocos</i> from <i>S. lineatus</i> off Lizard Island (15 replicates) and <i>P. munozae</i> <b>n. sp.</b> from <i>C</i>. <i>venustus</i> off Heron Island (four replicates) there are seven bases different.</p>Published as part of <i>Nolan, Matthew J. & Cribb, Thomas H., 2006, An exceptionally rich complex of Sanguinicolidae von Graff, 1907 (Platyhelminthes: Trematoda) from Siganidae, Labridae and Mullidae (Teleostei: Perciformes) from the Indo-west Pacific Region, pp. 1-80 in Zootaxa 1218 (1)</i> on pages 35-38, DOI: 10.11646/zootaxa.1218.1.1, <a href="http://zenodo.org/record/5064858">http://zenodo.org/record/5064858</a>
Phthinomita littlewoodi Nolan & Cribb 2006, n. sp.
No full textPhthinomita littlewoodi n. sp. (Figs. 38–40) Type host: Siganus lineatus (Valenciennes), Goldlined Rabbitfish (Perciformes: Siganidae). Other hosts: S. corallinus (Valenciennes), Coral Rabbitfish (Perciformes: Siganidae). Site in host: Intertrabecular spaces of ventricle (heart). Type locality: Heron Island, southern Great Barrier Reef (23°27'S 151°55'E), Queensland. Other localities: Lizard Island, northern Great Barrier Reef (14°40'S 145°27'E), Queensland. Material examined: ex S. lineatus, Heron Island (QLD), Apr. 2001, Feb. 2002, May. 2003, fifteen specimens (Holotype no. QM G 225607; Paratype nos. QM G 225608–225611); ex S. corallinus, Lizard Island (QLD), Aug. 2002, May 2004, six specimens (Paratype no. QM G 225612). Collector: M.J. Nolan. Etymology Specific name for Dr Timothy Littlewood of the Natural History Museum, London, in honour of his contributions to the field of platyhelminth phylogenetics. Description Based on 21 whole mounts. With features of genus. Body not curving dorsally posteriorly, slightly notched at male pore. Intestine; posterior caeca irregular in outline, distal extremities expanded. Anterior testis originates posterior to posterior caeca, margins lobed. Posterior testis elliptical, margins lobed. Cirrussac tearshaped to spherical. Internal seminal vesicle spherical or ovoid, occupying centre of cirrussac; ejaculatory duct sinuous; prostatic cells not seen. Ovary ovoid to spherical, posterior to posterior margin of anterior testis, medial. Oviduct originating at centre of posterior margin of ovary. Vitelline duct forming posterior to posterior margin of ovary, sinuous; vitelline reservoir forming anterior to anterior margin of cirrussac, convoluted, entering oötype posterodorsally. Oötype spherical, medial. Mehlis' gland extending anteriorly to posterior margin of cirrussac, extending posteriorly to anterior margin of posterior testis. Uterus extending from oötype, sinuous. Uterine chamber forming posterior to posterior margin of ovary, straight, thin. Vitelline follicles extending anteriorly past intestinal bifurcation, extending posteriorly past posterior margin of uterine chamber, filling intercaecal field, posteriorly passing medially. Remarks Phthinomita littlewoodi agrees well with the characters of Phthinomita and is distinguished by having the combination of a body 2993–4133 (3465) x 64–122 (94) with only a slight notch level with the male genital pore (by comparison to that of P. brooksi), an oesophagus that extends 24–42% of the body length, an anterior testis that is positioned posteriorly to the posterior caeca and occupies 16–31% of the body length, an anterior testis 4.4–12.4 times longer than the posterior testis, a tearshaped cirrussac, a uterine chamber 107–161 (127) long and vitelline follicles that extend anteriorly past the intestinal bifurcation. There are in addition, 1–33 base differences (0.3–10.0% sequence divergence) between the ITS2 rDNA sequence of P. littlewoodi and the remaining Phthinomita species sequenced here.Published as part of Nolan, Matthew J. & Cribb, Thomas H., 2006, An exceptionally rich complex of Sanguinicolidae von Graff, 1907 (Platyhelminthes: Trematoda) from Siganidae, Labridae and Mullidae (Teleostei: Perciformes) from the Indo-west Pacific Region, pp. 1-80 in Zootaxa 1218 (1) on pages 50-52, DOI: 10.11646/zootaxa.1218.1.1, http://zenodo.org/record/506485
Phthinomita jonesi Nolan & Cribb 2006, n. sp.
No full textPhthinomita jonesi n. sp. (Figs. 35–37) Type host: Siganus lineatus (Valenciennes), Goldlined Rabbitfish (Perciformes: Siganidae). Other hosts: S. argenteus (Quoy & Gaimard), Forktail Rabbitfish; S. doliatus (Cuvier), Barred Rabbitfish; S. vulpinus (Schlegel & Müller), Foxface. (Perciformes: Siganidae). Site in host: Intertrabecular spaces of ventricle (heart). Type locality: Lizard Island, northern Great Barrier Reef (14°40'S 145°27'E), Queensland. Material examined: ex S. lineatus, Lizard Island (QLD), Aug. 2002, May. 2004, thirtyfour specimens (Holotype no. QM G 225580; Paratype nos. QM G 225581–225589); ex S. argenteus, Lizard Island (QLD), Jan. 2003, (six specimens sequenced for ITS2); ex S. doliatus, Lizard Island (QLD), Jul. 2002, Aug. 2002, twentyfive partial and complete specimens (Paratype nos. QM G 225590–225602); ex S. vulpinus, Lizard Island (QLD), Aug. 2002, Jan. 2003, five specimens (Paratype nos. QM G 225603–225606). Collector: M.J. Nolan. Etymology Specific name for our friend Mr Conor Jones for his help on a collecting trip to Heron Island. Description Based on 64 partial and complete whole mounts. With features of genus. Body not curving dorsally posteriorly, slightly notched at male pore. Intestine; posterior caeca irregular in outline, distal extremities expanded. Anterior testis originates posterior to intercaecal field, in posterior third of body, margins lobed. Posterior testis ovoid, margins lobed. Cirrussac spherical to tearshaped. Internal seminal vesicle spherical, occupying ventral region of cirrussac; ejaculatory duct sinuous; prostatic cells small. Ovary ovoid, posterior to posterior margin of anterior testis. Oviduct originating at posterior margin of ovary, passing posterodorsally, straight. Vitelline duct forming between anterior margin of cirrussac and posterior margin of ovary, sinuous, dextral to cirrussac and posterior margin of ovary; vitelline reservoir forming lateral to cirrussac, convoluted, entering oötype posterodorsally. Oötype spherical, medial. Mehlis' gland extending anteriorly to posterior margin of cirrussac, extending posteriorly to anterior margin of posterior testis. Uterus extending from oötype sinuously, ventral to oviduct. Uterine chamber forming posterior to posterior margin of ovary, straight, thin, narrowing posteriorly before female genital pore. Vitelline follicles extending anteriorly past intestinal bifurcation, extending posteriorly past anterior margin of uterine chamber, filling intercaecal field, passing posteriorly dorsodextrally to anterior testis, anterior to anterior margin of ovary passes ventrally. Remarks The present material clearly belongs in the genus Phthinomita as it possesses characters differentially diagnostic of the genus. Phthinomita jonesi is distinguished by the combination of a body without a dorsal curve and only a slight notch level with the male pore, a body that is 45.8 times longer than wide on average, a spherical cirrussac, a uterine chamber that is 138 x 13 on average and vitelline follicles that extend anteriorly past the intestinal bifurcation. There are in addition, 1–34 base differences (0.3–10.3% sequence divergence) between the ITS2 rDNA sequence of P. jonesi and the remaining Phthinomita species sequenced here. There is a single base different between the sequences from P. jonesi (25 replicates), from four sympatric host species off Lizard Island and P. littlewoodi (eight replicates) from S. lineatus (Heron Island) and S. corallinus (Lizard Island).Published as part of Nolan, Matthew J. & Cribb, Thomas H., 2006, An exceptionally rich complex of Sanguinicolidae von Graff, 1907 (Platyhelminthes: Trematoda) from Siganidae, Labridae and Mullidae (Teleostei: Perciformes) from the Indo-west Pacific Region, pp. 1-80 in Zootaxa 1218 (1) on pages 48-50, DOI: 10.11646/zootaxa.1218.1.1, http://zenodo.org/record/506485
Phthinomita robertsthomsoni Nolan & Cribb 2006, n. sp.
No full textPhthinomita robertsthomsoni n. sp. (Figs. 41–43) Type host: Siganus argenteus (Quoy & Gaimard), Forktail Rabbitfish (Perciformes: Siganidae). m; 42, 100 m. Site in host: Intertrabecular spaces of ventricle (heart). Type locality: Lizard Island, northern Great Barrier Reef (14°40'S 145°27'E), Queensland. Material examined: ex S. argenteus, Lizard Island (QLD), Aug. 2002, Jan. 2003, twentytwo specimens (Holotype no. QM G 225613; Paratype nos. QM G 225614–225622). Collector: M.J. Nolan. Etymology Specific name for our good friend and colleague Mr Ashley RobertsThomson who collected the first S. argenteus of this study. Description Based on 22 whole mounts. With features of genus. Body notched at male genital pore. Intestine; posterior caeca sinuous. Anterior testis originating at posterior end of intercaecal field, margins lobed. Posterior testis tearshaped. Cirrussac tearshaped. Internal seminal vesicle spherical, occupying most of cirrussac, positioned ventrally; ejaculatory duct straight; prostatic cells large. Ovary oblanceolate, overlapping posterior margin of anterior testis. Oviduct originating at centre of posterior margin of ovary, sinuous, dorsal to vas deferens, entering oötype posterodorsally. Vitelline duct forming anterior to cirrussac, passing posteriorly sinuously, dextral to vas deferens; vitelline reservoir forming anterior to anterior margin of cirrussac, entering oötype posterodextrally. Oötype ovoid. Mehlis' gland extending anteriorly to posterior margin of cirrussac, extending posteriorly to midsection of posterior testis. Uterus extending from oötype, slightly sinuous, sinistral to oviduct. Uterine chamber forming anterior to posterior margin of ovary and anterior testis, ventral to ovary, sinuous, curving dorsally posteriorly to female pore. Vitelline follicles extending anteriorly past intestinal bifurcation, extending posteriorly to level female genital pore, sinistral and dextral to oesophagus, filling intercaecal field, dextral to anterior testis. Remarks This species possesses the characters differentially diagnostic of the genus Phthinomita and is here identified as a new species in that genus. Phthinomita robertsthomsoni is immediately distinguished by the combined possession of a body 3784–5706 (4851) long, a body 192 wide on average, an anterior testis 38–62% of the body length and 14.8–31.3 times longer than the posterior one, a posterior testis 15–46% of the body width, a cirrussac 64–96 (78) x 45–80 (64), an ovary 87–183 (138) x 64–144 (103) and a uterine chamber 257–385 (319) x 39–80 (59). The species differs further in having an anterior testis with the anterior margin in the posterior end of the intercaecal field, an ovary with an anterior margin lateral to the posterior margin of the anterior testis, a uterine chamber originating anteriorly to the posterior margin of both the anterior testis and the ovary and vitelline follicles extending anteriorly past the intestinal bifurcation. There are in addition, 20–29 base differences (5.8–8.6% sequence divergence) between the ITS2 rDNA sequence of P. robertsthomsoni and the remaining Phthinomita species sequenced here. Between the sequences from P. robertsthomsoni (two replicates) from S. argenteus off Lizard Island and P. sasali (one replicate) from S. doliatus off Palau there are 20 base differences.Published as part of Nolan, Matthew J. & Cribb, Thomas H., 2006, An exceptionally rich complex of Sanguinicolidae von Graff, 1907 (Platyhelminthes: Trematoda) from Siganidae, Labridae and Mullidae (Teleostei: Perciformes) from the Indo-west Pacific Region, pp. 1-80 in Zootaxa 1218 (1) on pages 52-55, DOI: 10.11646/zootaxa.1218.1.1, http://zenodo.org/record/506485
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