184 research outputs found
APPF TPA phenotyping dataset: UA (Gilliham, Qu) - Soybean
No full textImages and data from soybean phenotyping studies performed at the APPF Plant Accelerator (TPA), University of Adelaide, on behalf of UA (Gilliham, Qu) ending 2017-07-25</p
APPF TPA phenotyping dataset: UA (Gilliham, Pearson) - Wheat
No full textImages and data from wheat phenotyping studies performed at the APPF Plant Accelerator (TPA), University of Adelaide, on behalf of UA (Gilliham, Pearson) ending 2017-06-08</p
Author reponse: Plant trans-golgi network/early endosome pH regulation requires Cation Chloride Cotransporter (CCC1)
No full textThis is the Author response to article:Plant trans-Golgi network/early endosome pH regulation requires cation chloride cotransporter (CCC1) found at DOI: 10.7554/eLife.70701.Abstract not availableDaniel W McKay, Heather E McFarlane, Yue Qu, Apriadi Situmorang,
Matthew Gilliham, Stefanie Weg
Calcium compartmentation in arabidopsis mesophyll cells, a mechanism to regulate apoplastic calcium, photosynthetic rates and growth, involves low-affinity, high-capacity Ca2+/H+ antiporters
No full textSimon Conn, Matthew Gilliham, Brent Kaiser, Steve Tyerman and Roger Leighhttp://escholarship.org/uc/ipnc_xv
Barley phosphate transporter 1;6 shows broad inorganic anion transport activity when expressed in Xenopus laevis oocytes
No full textXVI IPNC 2009, Sacramento, CaliforniaPreuss, Christian P., Huang, Chun Y., Gilliham, Matthew and Tyerman, Stephen D.http://escholarship.org/uc/ipnc_xv
The evolutionary origin of CIPK16: a gene involved in enhanced salt tolerance
No full textAbstract not availableShanika Amarasinghe, Nathan S. Watson-Haigh, Matthew Gilliham, Stuart Roy, Ute Bauman
Cytosolic GABA inhibits anion transport by wheat ALMT1
No full textFirst published: 07 October 2019.
Corrected by: Corrigendum: Cytosolic GABA inhibits transport by wheat ALMT1 (vol 225, pg 671, 2020), in Early view - https://doi.org/10.1111/nph.18214. Since its publication, the authors of Long et al. (2020) have brought to our attention an error in their article. In the Acknowledgements section, the support for the work from the Australian Research Council Centre of Excellence funding to Matthew Gilliham and Stephen Tyerman was incorrectly listed under the number ‘CE14010008’. The correct funding number is ‘CE140100008’, and the corrected Acknowledgements section is shown below. We apologize to our readers for this mistake.Anion transport by Aluminium-activated malate transporter (ALMT) proteins is negatively regulated by gamma-aminobutyric acid (GABA), which increases in concentration during stress. Here, the interaction between GABA and wheat (Triticum aestivum, Ta) TaALMT1 heterologously-expressed in Xenopus laevis oocytes was investigated. GABA inhibited anion transport by TaALMT1 in membrane patches from the cytosolic, not extracellular membrane face, via a reduction in open probability (NPopen ), not an inhibition of channel current magnitude. TaALMT1 currents in patches frequently exhibited rundown with complete removal of cytosolic factors, but were partially sustained by protein kinase C dependent phosphorylation. When applied to whole oocytes a GABA-analogue-BODIPY conjugate inhibited TaALMT1 anion currents from the cytoplasmic face only, whereas free GABA inhibited from both the inside and outside consistent with GABA traversing the TaALMT1 pore then acting from the inside. We propose GABA does not competitively inhibit ALMT conductance through the same pore but rather leads to an allosteric effect, reducing anion channel opening frequency. Across plants GABA is a conserved regulator of anion transport via ALMTs - a family with numerous physiological roles beyond Al3+ tolerance. Our data suggests that a GABA-ALMT interaction from the cytosolic face has the potential to form part of a novel plant signalling pathway.Yu Long, Stephen D. Tyerman, Matthew Gilliha
Chloride: not simply a 'cheap osmoticum', but a beneficial plant macronutrient
No full textChloride is a nutrient that accumulates to millimolar levels in plants under most growth conditions, including in almost all soil-grown plants. These relatively high chloride concentrations (relative to the demand for chloride in photosynthesis) are beneficial to plants including non-halophytes, as the addition of chloride to the growth medium above the micromolar level increases biomass. As chloride is not metabolized and its only known essential function is in the oxygen-evolving complex in PSII, we discuss how chloride could be beneficial, especially in comparison with nitrate. We review the different routes taken by chloride in plants, from uptake and translocation to the shoot, and inside the cell in different organelles, including different transport mechanisms and the proteins identified. As the selectivity of many proteins to chloride and nitrate is not well established, the mechanisms within proteins to achieve selectivity of one anion over the other are explored. We further discuss the role of chloride as an osmoticum, why it might be preferentially used instead of other anions, and how chloride content in plants might beneficially influence nitrogen use efficiency and water-holding capacity.Stefanie Wege, Matthew Gilliham and Sam W. Henderso
Plant High-Affinity Potassium (HKT) transporters involved in salinity tolerance: structural insights to probe differences in ion selectivity
No full textHigh-affinity Potassium Transporters (HKTs) belong to an important class of integral membrane proteins (IMPs) that facilitate cation transport across the plasma membranes of plant cells. Some members of the HKT protein family have been shown to be critical for salinity tolerance in commercially important crop species, particularly in grains, through exclusion of Na+ ions from sensitive shoot tissues in plants. However, given the number of different HKT proteins expressed in plants, it is likely that different members of this protein family perform in a range of functions. Plant breeders and biotechnologists have attempted to manipulate HKT gene expression through genetic engineering and more conventional plant breeding methods to improve the salinity tolerance of commercially important crop plants. Successful manipulation of a biological trait is more likely to be effective after a thorough understanding of how the trait, genes and proteins are interconnected at the whole plant level. This article examines the current structural and functional knowledge relating to plant HKTs and how their structural features may explain their transport selectivity. We also highlight specific areas where new knowledge of plant HKT transporters is needed. Our goal is to present how knowledge of the structure of HKT proteins is helpful in understanding their function and how this understanding can be an invaluable experimental tool. As such, we assert that accurate structural information of plant IMPs will greatly inform functional studies and will lead to a deeper understanding of plant nutrition, signalling and stress tolerance, all of which represent factors that can be manipulated to improve agricultural productivity.Shane Waters, Matthew Gilliham and Maria Hrmov
Green horizons: how plant synthetic biology can enable space exploration and drive on Earth sustainability
No full textAs humanity looks towards expanding activity from low Earth orbit to the Moon and beyond, resource use efficiency and self-sustainability will be critical to ensuring success in the long term. Furthermore, solutions developed for the stringent requirements of space will be equally valuable in meeting sustainability goals here on Earth. Advances in synthetic biology allow us to harness the complex metabolism of life to produce the materials we need in situ. Translating those lessons learned from microbial systems to more carbon-efficient photosynthetic organisms is an area of growing interest. Plants can be engineered to sustainably meet a range of needs, from fuels to materials and medicines.Matthew Fox Morgan, Jonathan Diab, Matthew Gilliham, and Jenny C Mortime
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