985 research outputs found

    Image of Matt Turner, Ross Garner and Shelley Jones before interview (3 of 4)

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    Matt poured himself a coca-cola, and eyes me photographing while Ross and Shelley finish setting up for the interview

    Portrait of Matt Garner

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    This black and white photograph features an individual portrait of Matt Garner.https://scholars.fhsu.edu/mens_basketball_photos/1587/thumbnail.jp

    Standing Portrait of Matt Garner

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    This black and white photograph features an individual portrait of Matt Garner.https://scholars.fhsu.edu/mens_basketball_photos/1613/thumbnail.jp

    Bsubtilis bMD210

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    Strain from Ethan garner. It is a PonA mutant with Peter Graumann's GFP-Mbl contruct.This info was provided by Mike Dion in Ethan's lab:(Ω) cat::Pxyl-gfp-mbl ΔponA::kan<strong>Tilt Series Date:</strong> 2013-12-01</p> <strong>Data Taken By:</strong> Matt Swulius</p> <strong>Species / Specimen:</strong> Bacillus subtilis </p> <strong>Strain:</strong> bMD210, PonA null, GFP-Mbl</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p> <strong>Microscope:</strong> Caltech Polara</p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Collaborators and Roles:</strong> Ethan Garner</p> <strong>Purification / Growth Conditions / Treatment:</strong> Sample were grown in LB + 5 um Chloramphenicol + 10 mM MgCl2 + 30 mM xyl. 10 ml grown in 100 ml baffled flask for best aeration according to Ethan.</p> <strong>Sample Preparation:</strong> Frozen in vitrobot, but manually from behind. Cells were grown to ~ OD of 0.3 then diluted 10 fold.</p> mst2013-12-01-3_slicer4722.jpg: nested vesicleFiles available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-3</p>120113_bs2100003.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-3/rawdata/120113_bs2100003.mrc" > <i class="download icon"></i> Download </a></p> 120113_bs2100003_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-3/3dimage_35283/120113_bs2100003_full.rec" > <i class="download icon"></i> Download </a></p&gt

    Bsubtilis bMD210

    No full text
    Strain from Ethan garner. It is a PonA mutant with Peter Graumann's GFP-Mbl contruct.This info was provided by Mike Dion in Ethan's lab:(Ω) cat::Pxyl-gfp-mbl ΔponA::kan<strong>Tilt Series Date:</strong> 2013-12-01</p> <strong>Data Taken By:</strong> Matt Swulius</p> <strong>Species / Specimen:</strong> Bacillus subtilis </p> <strong>Strain:</strong> bMD210, PonA null, GFP-Mbl</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p> <strong>Microscope:</strong> Caltech Polara</p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Collaborators and Roles:</strong> Ethan Garner</p> <strong>Purification / Growth Conditions / Treatment:</strong> Sample were grown in LB + 5 um Chloramphenicol + 10 mM MgCl2 + 30 mM xyl. 10 ml grown in 100 ml baffled flask for best aeration according to Ethan.</p> <strong>Sample Preparation:</strong> Frozen in vitrobot, but manually from behind. Cells were grown to ~ OD of 0.3 then diluted 10 fold.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-13</p>120113_bs2100013.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-13/rawdata/120113_bs2100013.mrc" > <i class="download icon"></i> Download </a></p> 120113_bs2100013_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-13/3dimage_35299/120113_bs2100013_full.rec" > <i class="download icon"></i> Download </a></p&gt

    Bsubtilis bMD210

    No full text
    Strain from Ethan garner. It is a PonA mutant with Peter Graumann's GFP-Mbl contruct.This info was provided by Mike Dion in Ethan's lab:(Ω) cat::Pxyl-gfp-mbl ΔponA::kan<strong>Tilt Series Date:</strong> 2013-12-01</p> <strong>Data Taken By:</strong> Matt Swulius</p> <strong>Species / Specimen:</strong> Bacillus subtilis </p> <strong>Strain:</strong> bMD210, PonA null, GFP-Mbl</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p> <strong>Microscope:</strong> Caltech Polara</p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Collaborators and Roles:</strong> Ethan Garner</p> <strong>Purification / Growth Conditions / Treatment:</strong> Sample were grown in LB + 5 um Chloramphenicol + 10 mM MgCl2 + 30 mM xyl. 10 ml grown in 100 ml baffled flask for best aeration according to Ethan.</p> <strong>Sample Preparation:</strong> Frozen in vitrobot, but manually from behind. Cells were grown to ~ OD of 0.3 then diluted 10 fold.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-14</p>120113_bs2100014.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-14/rawdata/120113_bs2100014.mrc" > <i class="download icon"></i> Download </a></p> 120113_bs2100014_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-14/3dimage_35301/120113_bs2100014_full.rec" > <i class="download icon"></i> Download </a></p&gt

    Bsubtilis bMD210

    No full text
    Strain from Ethan garner. It is a PonA mutant with Peter Graumann's GFP-Mbl contruct.This info was provided by Mike Dion in Ethan's lab:(Ω) cat::Pxyl-gfp-mbl ΔponA::kan<strong>Tilt Series Date:</strong> 2013-12-01</p> <strong>Data Taken By:</strong> Matt Swulius</p> <strong>Species / Specimen:</strong> Bacillus subtilis </p> <strong>Strain:</strong> bMD210, PonA null, GFP-Mbl</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p> <strong>Microscope:</strong> Caltech Polara</p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Collaborators and Roles:</strong> Ethan Garner</p> <strong>Purification / Growth Conditions / Treatment:</strong> Sample were grown in LB + 5 um Chloramphenicol + 10 mM MgCl2 + 30 mM xyl. 10 ml grown in 100 ml baffled flask for best aeration according to Ethan.</p> <strong>Sample Preparation:</strong> Frozen in vitrobot, but manually from behind. Cells were grown to ~ OD of 0.3 then diluted 10 fold.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-5</p>120113_bs2100005.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-5/rawdata/120113_bs2100005.mrc" > <i class="download icon"></i> Download </a></p> 120113_bs2100005_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-5/3dimage_35287/120113_bs2100005_full.rec" > <i class="download icon"></i> Download </a></p&gt

    Bsubtilis bMD210

    No full text
    Strain from Ethan garner. It is a PonA mutant with Peter Graumann's GFP-Mbl contruct.This info was provided by Mike Dion in Ethan's lab:(Ω) cat::Pxyl-gfp-mbl ΔponA::kan<strong>Tilt Series Date:</strong> 2013-12-01</p> <strong>Data Taken By:</strong> Matt Swulius</p> <strong>Species / Specimen:</strong> Bacillus subtilis </p> <strong>Strain:</strong> bMD210, PonA null, GFP-Mbl</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p> <strong>Microscope:</strong> Caltech Polara</p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Collaborators and Roles:</strong> Ethan Garner</p> <strong>Purification / Growth Conditions / Treatment:</strong> Sample were grown in LB + 5 um Chloramphenicol + 10 mM MgCl2 + 30 mM xyl. 10 ml grown in 100 ml baffled flask for best aeration according to Ethan.</p> <strong>Sample Preparation:</strong> Frozen in vitrobot, but manually from behind. Cells were grown to ~ OD of 0.3 then diluted 10 fold.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-12</p>120113_bs2100012.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-12/rawdata/120113_bs2100012.mrc" > <i class="download icon"></i> Download </a></p> 120113_bs2100012.rec, Reconstruction (Pixel Size 1.92 nm), 1.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-12/3dimage_35363/120113_bs2100012.rec" > <i class="download icon"></i> Download </a></p&gt

    Bsubtilis bMD210

    No full text
    Strain from Ethan garner. It is a PonA mutant with Peter Graumann's GFP-Mbl contruct.This info was provided by Mike Dion in Ethan's lab:(Ω) cat::Pxyl-gfp-mbl ΔponA::kan<strong>Tilt Series Date:</strong> 2013-12-01</p> <strong>Data Taken By:</strong> Matt Swulius</p> <strong>Species / Specimen:</strong> Bacillus subtilis </p> <strong>Strain:</strong> bMD210, PonA null, GFP-Mbl</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p> <strong>Microscope:</strong> Caltech Polara</p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Collaborators and Roles:</strong> Ethan Garner</p> <strong>Purification / Growth Conditions / Treatment:</strong> Sample were grown in LB + 5 um Chloramphenicol + 10 mM MgCl2 + 30 mM xyl. 10 ml grown in 100 ml baffled flask for best aeration according to Ethan.</p> <strong>Sample Preparation:</strong> Frozen in vitrobot, but manually from behind. Cells were grown to ~ OD of 0.3 then diluted 10 fold.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-7</p>120113_bs2100007.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-7/rawdata/120113_bs2100007.mrc" > <i class="download icon"></i> Download </a></p> 120113_bs2100007_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-7/3dimage_35364/120113_bs2100007_full.rec" > <i class="download icon"></i> Download </a></p&gt

    Bsubtilis bMD210

    No full text
    Strain from Ethan garner. It is a PonA mutant with Peter Graumann's GFP-Mbl contruct.This info was provided by Mike Dion in Ethan's lab:(Ω) cat::Pxyl-gfp-mbl ΔponA::kan<strong>Tilt Series Date:</strong> 2013-12-01</p> <strong>Data Taken By:</strong> Matt Swulius</p> <strong>Species / Specimen:</strong> Bacillus subtilis </p> <strong>Strain:</strong> bMD210, PonA null, GFP-Mbl</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p> <strong>Microscope:</strong> Caltech Polara</p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Collaborators and Roles:</strong> Ethan Garner</p> <strong>Purification / Growth Conditions / Treatment:</strong> Sample were grown in LB + 5 um Chloramphenicol + 10 mM MgCl2 + 30 mM xyl. 10 ml grown in 100 ml baffled flask for best aeration according to Ethan.</p> <strong>Sample Preparation:</strong> Frozen in vitrobot, but manually from behind. Cells were grown to ~ OD of 0.3 then diluted 10 fold.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-4</p>120113_bs2100004.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-4/rawdata/120113_bs2100004.mrc" > <i class="download icon"></i> Download </a></p> 120113_bs2100004_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-01-4/3dimage_35281/120113_bs2100004_full.rec" > <i class="download icon"></i> Download </a></p&gt
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