4 research outputs found

    Biochemical Alterations in the Haemolymph of Silkworm [Bombyx mori (L).(Lepidoptera: Bombycidae)] Fed with Mulberry Leaves Enriched with Indian Bean (Dolichos lablab)

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    A study was carried out to evaluate the effect of Indian bean (Dolichos lablab) supplementation on silkworm. Finely powdered Dolichos lablab was dissolved in distilled water and diluted to 2.5 %, 5 %, 7.5 % and 10 % concentrations. Fresh mulberry leaves (Morus alba L.) were sprayed by each concentration and were fed to silkworms, from 3rd to 5th instar, five feedings/day. Group 1 larvae received mulberry leaves sprayed with distilled water and served as control, group 2  larvae received 2.5% Dolichos lablab sprayed mulberry leaves, group 3 larvae received 5 % Dolichos lablab sprayed mulberry leaves, group 4 larvae received 7.5 % Dolichos lablab sprayed mulberry leaves and group 5 larvae received 10 % Dolichos lablab sprayed mulberry leaves. Silkworm larvae fed on Morus alba. L. (mulberry) leaves enriched with 7.5 % concentrations of Dolichos lablab, significantly gained more Cocoon weight, Shell weight and Shell/cocoon ratio as compared to those fed on normal MR2 mulberry leaves. Hence, 7.5% dose was fixed as an effective dose. Further, same study was conducted to find out the biochemical changes in the haemolymph occurred in the first day of Vth instar larvae. There was a significant increase in the haemolymph glucose, cholesterol, urea, total protein, aspartate transaminase, alanine transaminase and alkaline phosphatase. But haemolymph uric acid was significantly decreased. The results suggest that coadministration of   Dolichos lablab with mulberry leaves at a concentration of 7.5% has enhanced the biochemical reaction involved in the silk production in the silkworm.  ÂÂ

    Studies on the Growth Rate of Silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae) Fed with Control and Silver Nanoparticles (AgNps) Treated MR2 Mulberry Leaves

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    To evaluate the growth rate of larval and pupal parameters of silkworm Bombyx mori fed with Silver Nanoparticles (AgNps) treated MR2 mulberry leaves, the following works have been considered. The AgNp was synthesized by chemical reduction method, it was diluted by different concentrations such as 25%, 50%, 75 % and 100 % (without dilution). Fresh mulberry leaves (Morus alba L.) were sprayed by each concen-tration and were fed to silkworms, from 3rd, 4th and 5th instar, five feedings/day. Group T1 larvae received MR2 mulberry leaves sprayed with distilled water and served as control, group T2, T3, T4 and T5 larvae received 25%, 50%, 75 % and 100 % AgNps sprayed mulberry leaves, respectively. Silkworm larvae fed on M. alba (MR2) leaves sprayed with 25 % concentration of AgNps (group T2) was significantly increased the larvae and cocoon length, width and weight as com-pared to those fed on control (group T1) MR2 mul-berry leaves and other groups (T3, T4 and T5). Hence, 25 % AgNps dose was fixed as an effective dose. It has been observed from the present study that 25 % AgNps treated (group T2) leaves fed by silkworms have enhanced the larval and pupal growth and quantity of silk production than control
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