34 research outputs found

    SSIIbis - Contre-projet pour une nouvelle usine d’eau potable à Saint-Sulpice

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    40% de l’agglomération lausannoise boit l’eau du lac Léman puisée à Saint-Sulpice, zone à faible densité avec un revenu moyen élevé, et la rejette par la station d’épuration de Vidy, lieu de loisirs populaires et de transit. L’usine d’eau potable de Saint-Sulpice est considérée comme obsolète. Une étude menée sur plusieurs années par un groupe de spécialistes a défini le processus de filtration et son un site implantation sur la parcelle. Devisé à 82 millions de francs, le projet est passé aujourd’hui à 125 millions. En 2020, un concours SIA 142 appelle les architectes à proposer une « enveloppe » pour le processus, tout en conservant le parc, accessible au public depuis le sentier de la rive. L’administration des ressources nécessite un nombre important de décisions politico-bureaucratiques qui impactent la population régionale. Cette infrastructure critique contient un potentiel important d’appropriation et de confrontation politique qui peut être reversé dans un projet d’architecture. Ma proposition questionne donc la démolition de l’usine de Saint-Sulpice, parée de marbre et structurellement en bon état, qui peut servir d’accueil à une contre-culture active dans les centralités urbaines, où les espaces à disposition diminuent. L’usine projetée établit un rapport dense avec l’existant et permet une promenade le long des étapes de filtration. La conservation des traces des excavations nécessaires au chantier crée une nouvelle topographie, un rez-de-chaussée extérieur, où les procédés techniques de traitement de l’eau normalement enfouis sont désormais visibles.LDMLASURSAR-DCote: 2022.085Archive: MEM.1/1 A4 verticalGroupe de suivi: Huang, Jeffrey (dir. pédagogique) ; Pattaroni, Luca (prof.) ; Ledermann, Gianna (maître EPFL) ; Szacka, Léa-Catherine (expert)Professeur responsable de l'Enoncé: Pattaroni, Luca (ENAC IA LASUR)Enoncé théorique de master: Corps en action et production d'espaces de contestation.Prix des meilleurs Enoncés théoriques de maste

    Expression and subcellular localization of GPR3 during porcine oocyte meiotic maturation.

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    <p>(A) Subcellular localization of GPR3 as revealed by immunofluorescent staining. In the GV stage, GPR3 was mainly distributed at the nuclear membrane and plasma membrane. GPR3 accumulated at the inner cytoplasm and plasma membrane at the pro-MI stage. From MI to MII, GPR3 aggregated at the plasma membrane. (B) Expression of GPR3 protein was measured by western blotting. Samples were collected at 0 h, 24 h, 30 h, and 44 h of culture which were the time points when most oocytes had reached the GV, GVBD, MI and MII stages, respectively. Each sample was derived from 150 oocytes. Green, GPR3; Blue, chromatin. Bar = 40 µm.</p

    CCDC 297698: Experimental Crystal Structure Determination

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    Related Article: A.Noor, W.Kretschmer, R.Kempe|2006|Eur.J.Inorg.Chem.||2683|doi:10.1002/ejic.200600120,An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

    Aprender juntos. La escucha activa del otro: comunicación verbal y comunicación no verbal entre un tutor y un alumno

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    L'objectiu d'aquest treball és analitzar l'expressió d'actituds assertives i no assertives cap a l'alumne per part d'un tutor a través de la seva comunicació verbal i no verbal durant una entrevista, així com la reacció de l'alumne davant les actituds percebudes a través de la seva comunicació verbal i no verbal. Les categories d'anàlisi del tutor són: estructurar, establir concordança, transmetre informació i / o donar opinions i escoltar activament. Les categories analitzades en la reacció de l'alumne són: evadir la situació, proporcionar informació, expressar conflictes, acceptar compromisos i proposar solucions. En aquesta investigació qualitativa les dades s'obtenen mitjançant la tècnica de l'anàlisi de contingut del «Informe Individual» redactat per 124 alumnes de Grau de Mestre en Educació Primària i «l'Informe de 18 grups de discussió» formats per aquests alumnes» dels cursos 2009-2010 i 2012-2013. Es conclou la necessitat de formar el professorat en tècniques d'expressió del cos, del gest i de la veu, ja que un percentatge considerable d'aquest alumnat de magisteri tot just fonamenta la comunicació gestual i el parallenguatge en les seves percepcions assertives i no assertives tant del tutor com de l'alumne.The aim of this work is to analyse the expression of a counsellor’s assertive and non-assertive attitudes towards a student by observing his/her verbal communication and nonverbal communication during an interview, and by analysing the student’s reaction to these attitudes perceived by the student’s verbal and nonverbal communication. The categories used for the counsellor analysis are: to structure, to reach an agreement, to transmit information and/or give opinions, and to listen actively. The categories employed to analyse the student’s reaction are: to evade the problem, to provide information, to express conflicts, to make commitments and to propose solutions. In this qualitative research data are obtained applying the content analysis technique to 124 «Individual Reports» written by students of the Bachelor’s Degree in Primary Education and to 18 «Discussion Group Reports» formed by the same students during the academic years 2009-2010 and 2012-2013. We conclude that it is necessary to train teachers in body language, facial and intonation expression, because a considerable percentage of these Bachelor’s Degree in Education students scarcely back up their perceptions of the counsellor and student’s assertive and non-assertive attitudes with non-verbal and paralanguage observations.El objetivo de este trabajo consiste en analizar la expresión de actitudes asertivas y no asertivas hacia el alumno por parte de un tutor a través de su comunicación verbal y de comunicación no verbal durante una entrevista, así como el análisis de la reacción del alumno ante las actitudes percibidas a través de su comunicación verbal y de su comunicación no verbal. Las categorías de análisis del tutor son estructurar, establecer concordancia, transmitir información y/o dar opiniones, y escuchar activamente. Las categorías analizadas en la reacción del alumno son evadir la situación, proporcionar información, expresar conflictos, aceptar compromisos y proponer soluciones. En esta investigación cualitativa, los datos se obtienen mediante la técnica del análisis de contenido del «Informe Individual», redactado por 124 alumnos y alumnas de Grado de Maestro en Educación Primaria, y del «Informe de 18 grupos de discusión», formados por dichos alumnos de los cursos 2009-2010 a 2012-2013. Se concluye que es necesario formar al profesorado en técnicas de expresión del cuerpo, del gesto y de la voz, ya que un porcentaje considerable de este alumnado de magisterio apenas fundamenta la comunicación gestual y el paralenguaje en sus percepciones asertivas y no asertivas tanto del tutor como del alumno

    Model of IRM-protein interactions in the <i>Drosophila</i> anterior wing margin.

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    <p>(A-E) Illustration of IRM-protein functions in the anterior wing margin. In black are epithelial cells shown, while SOPs are shown in orange. Protein interactions are shown in different sizes according to the strength of the interaction. Red represents Rst, green Hbs, blue Kirre and SNS is shown in yellow. In the wild type (A) preferred adhesion was observed between the SOPs and the surrounding epithelial cells. Epithelial cells are additionally stable connected through the Hbs, Rst and Hbs, Kirre interaction. In <i>rst</i><sup><i>1R34</i></sup> (B), as an example for Neph-like loss of function, preferential adhesion can still be observed between the SOPs and epithelial cells through the SNS, Kirre and Hbs Kirre interaction. Only in the case of Rst and Kirre loss, the adhesive properties of the wing margin is changed leading to bristle clusters (C). Loss of Hbs prevents heterophilic interaction between the non-SOP cells resulting in mild disturbances of the SOP pattern (D). Loss of Hbs and SNS results in total loss of heterophilic interaction between all cell types in the presumptive anterior wing margin (E). This results in strong disturbances of the SOP and later the bristle pattern (F) Summary of the inductive and competitive interactions between the IRM-proteins <i>in trans</i> and <i>in cis</i>. In the interaction between two cells <i>in trans</i> several inductive events were observed, if these events represent inductions of gene expression or stabilization of proteins in the adhesive belt by heterophilic interactions is currently unknown. Inside cells <i>in cis</i> several competitive interactions were observed, resulting in degradation of proteins in vesicles. Altogether, these interactions allow a precise regulation of IRM-protein abundance and function. (G) Chain model of preferential adhesion of IRM-proteins in the wing disc. The IRM-proteins in the wing disc secure a strong adhesive chain in the distal growing wing. Preferential adhesion around the SOPs secures a constant high number of cells between the SOPs. Growth in distal directions explains the lower number of cells between precursors compared to the adult sensory organs.</p

    The Neph-like proteins Rst and Kirre affect the localization of other IRM members in <i>cis</i> and <i>trans</i>.

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    <p>(A-M, O, Q-X) Projection views of IRM immunoreactivity in third instar larvae. Rst is shown in red (A, E, I, M-Q and U-V), Hbs in green (B, F, J, M-P, R and U-V), Kirre in blue (C, G, K, S and W-Z) and SNS in yellow (D, H, L, T and W-Z). (A-D) Misexpression of <i>rst</i> using <i>MZ1369-GAL4</i> leads to ubiquitous Rst staining (A) in the entire wing disc. Hbs (B) and Kirre (C) are significantly reduced. SNS (D) staining is unaffected in strength, but the localization is not limited to the apical contact zone of the SOPs. Instead it is found in the entire cell. Additionally, the order of the SOPs is severely disturbed. (E-H) Misexpression of <i>kirre</i> via <i>MZ1369-GAL4</i> leads to wider stripes of Rst (E) and Hbs (F) staining. Kirre (G) can be ubiquitously detected in the entire wing disc. SNS (H) staining is strong on all membranes in contact with Kirre positive membranes. Spacing of SOPs is already disrupted at this developmental stage. (I-L) Misexpression of <i>rst</i> using <i>neur-GAL4</i> leads to strongly stained Rst (I) positive SOPs. Hbs (J) is found only around the SOPs and staining of membranes not in contact to the SOPs is reduced. (K) Kirre staining is further enriched around the SOPs. (L) SNS is relocated and it is not specifically located at the adherens junction any more. (M) Magnification of three SOPs of a wild type control stained for Rst and Hbs. The dashed line shows the approximate area of cut for the Z-projection in (N). Hbs can be detected inside the SOPs (arrowhead) and also in the basal appendix (arrow). (O) Magnification of three SOPs in <i>neur-GAL4</i>><i>UAS-rst</i>. The approximate area of cut for the Z-projection in (P) is given with a dashed line. Arrowheads mark the immunopositive interior of the SOPs showing strong Rst staining. Arrows marks the basal appendix of the SOPs. Asterisk mark the co-localization of Rst and Hbs immunoreactivity at the Border of SOPs. (Q-T) Misexpression of <i>kirre</i> using <i>neur-GAL4</i> leads to strong Rst staining around the SOPs. Hbs (R) is found much stronger around or in the SOPs and is strongly reduced on the membranes not in contact with any SOPs. (S) Kirre staining is strongly found in all membranes of the SOPs, showing no apical-basal polarity. (T) SNS localization inside the SOPs is disrupted and a possible degradation product can be found in vesicles basal of the adherens junction. (U) Magnification of three SOPs of a wild type control stained for Rst and Hbs. (V) Magnification of three SOPs in <i>neur-GAL4</i>><i>UAS-kirre</i>. Strong accumulation of Hbs immunoreactivity is evident around the SOPs. (W) Magnification of three SOPs of a wild type control stained for Kirre and SNS. (X) The magnification of three SOPs of <i>neur-GAL4</i>><i>UAS-kirre</i> shows the strong Kirre staining in the entire SOP and the mislocalization of SNS. A dotted line in (W and X) shows the approximate area of a Z-cut that is shown in (Y and Z). In wild type (Y) both proteins interact with each other only in a defined apical contact zone. The distribution of the proteins is clearly visible. SNS is inside the SOPs and Kirre in the surrounding cells. In (Z) the distribution of Kirre and SNS is shown in the mutant situation. Mislocalization and vesicular degradation products of SNS can be seen basal of the adherens junction in this z-axis view. Scale bars correspond to 10μm in all images.</p
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