1,088 research outputs found
Differential regulation of the phenazine biosynthetic operons by quorum sensing in Pseudomonas aeruginosa PAO1-N
No full textThe Pseudomonas aeruginosa quorum sensing (QS) network plays a key role in the adaptation to environmental changes and the control of virulence factor production in this opportunistic human pathogen. Three interlinked QS systems, namely las, rhl, and pqs, are central to the production of pyocyanin, a phenazine virulence factor which is typically used as phenotypic marker for analysing QS. Pyocyanin production in P. aeruginosa is a complex process involving two almost identical operons termed phzA1B1C1D1E1F1G1 (phz1) and phzA2B2C2D2E2F2G2 (phz2), which drive the production of phenazine-1-carboxylic acid (PCA) which is further converted to pyocyanin by two modifying enzymes PhzM and PhzS. Due to the high sequence conservation between the phz1 and phz2 operons (nucleotide identity > 98%), analysis of their individual expression by RNA hybridization, qRT-PCR or transcriptomics is challenging. To overcome this difficulty, we utilized luminescence based promoter fusions of each phenazine operon to measure in planktonic cultures their transcriptional activity in P. aeruginosa PAO1-N genetic backgrounds impaired in different components of the las, rhl, and pqs QS systems, in the presence or absence of different QS signal molecules. Using this approach, we found that all three QS systems play a role in differentially regulating the phz1 and phz2 phenazine operons, thus uncovering a higher level of complexity to the QS regulation of PCA biosynthesis in P. aeruginosa than previously appreciated
Book review: Contemporary Scottish plays, edited by Trish Reid
No full textBook review: Contemporary Scottish plays, edited by Trish Reid. London:
Bloomsbury, 2014; ISBN: 9781472574435 (£17.99)Publisher PD
Young Workers Cheer Evans, Fletcher.
No full textAt an Action for Washington rally, Fletcher received almost as many cheers for his primary victory as Evans
Art Fletcher Differs with Muncey on Post
No full textMuncey sees the position as PR, while Fletcher "would seek to carry out specific duties in the field of urban affairs." "The two will appear jointly on a television program on KING-TV, Seattle, Sept. 8." "Fletcher also said Friday" that he would be meeting with Nixon to discuss self-help programs "in New York early next month.
Correspondence
No full textMissing first 3 pages of same date/author/recipient to propose a 90 day Fletcher�s Learning System life management self sufficiency enhancement demonstration project in Black History Month 1994
The Research Potential and Significance of the Egyptian Collections within Yorkshire Museums
No full textThis research project addresses the Research Potential and Significance of Egyptian Material within
Yorkshire Museums. This scoping project ascertains what Egyptian material is present in Yorkshire,
particularly in storage, to open the material up for further study.
This material has been put under little or no examination in the past, with the exception of the ongoing
studies of Dr. J. Fletcher. There have been several cases, highlighted by Fletcher, of museums that hold a
significant and unique collection of Egyptian artefacts that were hitherto unknown, an example being
Harrogate Museums and Arts. This lack of knowledge is not only in the public domain but also present in
the knowledge of museums themselves.
Through highlighting the material that is present in Yorkshire, the project has identified specific areas of
research that can be expanded on. Research potential is discussed constantly throughout the thesis,
indicating how significant this material is.
This thesis also highlights the local significance of the material, which is a contentious issue as Egyptian
material is clearly not excavated within Yorkshire. The author has argued that once assimilated into
museum or private collections, the material becomes part of the local history.
This study records many items of individual interest for future study as well as addressing conditions in
which the materials are currently held. This includes consideration of material damage and corrosion, which
in some cases is ongoing and requires attention.
Public awareness has been a strong influence in the development of this research. Through studying and
highlighting this material, it is anticipated that museums can be encouraged to better utilise their
collections and to construct a foundation for further academic research. This research also comes at a
pertinent time, with museums being hard hit by funding and budget cuts, which are noted to have affected
the study and display of Egyptian material
Art Fletcher: Equal Employment Opportunity Commissioner
No full textSame as same date/author/recipient
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