1,720,982 research outputs found
Recenti acquisizioni nella diagnosi immunologica delle Malattie Infettive e Parassitarie
No full textIn the last years, remarkable advancements concerning the immunological diagnosis of infections and parasitic diseases have been made. We briefly review, also on the basis of our personal experience, the most recent immunological methods commonly employed for the diagnosis of viral, bacterial and parasitic diseases
Endocardite Batterica da Streptococco di Gruppo C. Considerazioni su un Caso Clinico
No full textGroup C streptococci cause commonly infections in animals; they can be found as saprophytes on human mucosae and may occasionally cause severe infections in man.
We report the case of a young woman affected by group C streptococcus endocarditis whose distinctive features were the presence of endocardial lesions only on the right ventricular wall and the rapid recovery
IMMUNOBLOT ANALYSIS OF ANTIBODY-RESPONSE TO SALMONELLA SPP AND ESCHERICHIA-COLI LIPOPOLYSACCHARIDES (LPS) IN TYPHOID PATIENTS
No full textThe Authors have applied the Immunoblot technique to perform an immunological analysis in order to detect and characterize the antibodies against the Salmonella Spp and Escherichia Coli (LPS) in patients with typhoid fever
Impiego del Dot Immunobinding assay (DIB) nella diagnosi della Schistosomiasi Urinaria
No full textA Dot Immnunobinding assay (DIB) has been applied to the serodiagnosis of urinary schistosomiasis and its resuls have been compared with those obtained with the Enzyme Linked Immunosorbent Assy (ELISA). The two techniques have been shown to be closely related (p<0.001). DIB however is more economical and can be read in less than 4 hours. It is simple to perform and easily adaptable for field use
Use of dot immunobinding assay for the rapid diagnosis of human hydatidosis.
No full textA Dot Immunobinding (DIB) assay has been applied to the serodiagnosis of human hydatidosis and its results have been compared with those obtained with the enzyme-linked immunosorbent assay (ELISA). The two techniques have been shown to be closely related (p greater than 0.001), highly sensitive (92.0% of positive results in 75 sera from patients with hepatic or pulmonary hydatidosis) and specific (93.5% of negative results in 31 sera from patients affected by other parasitic diseases and 100% of negative results in 30 normal controls). DIB however is more economical and takes less time (only 4 hours) than ELISA. DIB could be an useful tool in field epidemiological surveys since it is sensitive, specific, simple to perform and it does not require any expensive apparatus
USE OF DOT IMMUNOBINDING ASSAY (DIB) FOR THE RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS
No full textA new immunological method, the Dot Immunobinding Assay is employed for diagnosis of human Brucellosis. This procedure has been shown simple, rapid with high sensitivity and specificity
Sistemic antibody response to Salmonella typhi lipopolysaccharide in typhoid patients: evaluation by enzyme-linked immunosorbent assay and immunoblotting
No full textThe antibody response to the lypolysaccharide (LPS) of Salmonella typhi in patients with typhoid fever has been evaluated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Sera from eighteen typhoid patients, forty febrile non typhoidal patients and eighty healthy donors were tested for the presence of IgG, IgA and IgM anti LPS antibodies. IgG, IgA and IgM antibodies were higher for typhoid patients than for control patients; the detection of IgG anti-LPS antibodies by immunoblotting gave the most sensitive results; even the IgM test was more specific. Immunoblotting experiments showed that typhoid sera recognize various antigenic determinants located along the O-side chain as well as the core-lipid A region of the S. typhi LPS. Immunoblotting is a useful technique to investigate the humoral immune response to S. typhi LPS, since both antibody specificity and isotype distribution can be easily determined
Determinazione dell'attività adenoisina deaminasi (ADA) nel liquor di pazienti con infezione da HIV: un possibile marker di replicazione virale
No full textIn this paper, cerbrospinal fluid (CSF) adenosine deaminase activity has been measured in 15 patients with HIV infection (10 with AIDS, 2 wiith ARC and 3 with LAS), with and without neurological disorders, In addition, CSF samples from 22 patients with other cerebral diseases have been tested. Levels of CSF ADA activity were significantly increased in patients with HIV infection, as compared with controls. In one patient with AIDS-dementia complex, a progressive increase of CSF ADA activity with the parallel development of neurologic symptoms has been found. Our results suggest that CSF ADA activity measurement could be a possible marker of HIV replication in the central nervous system
Myelin degrading activity in the CSF of HIV-1-infected patients with neurological diseases
No full textDegradation of purified myelin basic protein (MBP) was studied by SDS gel electrophoresis after addition of CSF samples obtained from HIV-1-infected patients. An increase in MBP degradation was detected in patients with neurological complications, such as AIDS dementia complex (ADC) or progressive multifocal leukoencephalopathy (PML), when compared with patients with no neurological symptoms (NA) or with other neurological opportunistic infections (OI). In the ADC and PML patients, in addition to CSF proteolytic activity, an increase in CSF-MBP levels and presence of white matter lesions were also observed by neuroimaging (MRI). In other opportunistic infections of the brain, MBP levels but not anti-MBP proteolytic activity increased. Results suggest the involvement of proteases in the virus-induced demyelination
Nuove Prospettive diagnostiche dell'infezione da Pnumocystis carini
No full textNel corso di recenti ricerche sono emersi alcuni dati di rilevante interesse:
1- Evidenziazione di P. carinii in soggetti HIV positivi asintomatici ed HIV negativi esenti da infezioni respiratorie in atto (1). Sono in corso a tal proposito ricerche sistematiche volte a stabilire il significato del riscontro di P. carinii in secrezioni broncoalveolari di pazienti con o senza infezione da HIV, dell'eventuale presenza di stipiti differenti del microrganismo e del loro ruolo nella malattia;
2- Isolamento di P. carinii da cellule mononucleate di pazienti di AIDS con PCP, coltivate su linea A549 di derivazione umana (2). Tale sistema, che ha consentito di porre diagnosi di PCP in una vasta percentuale di casi, potrebbe rappresentare, se i risultati verranno confermati da più ampie casistiche, un approccio alternativo o complementare alla diagnosi dell'infezione da P. carinii.
3- Evidenziazione di alcune componenti ad attività proteasica (MW 100-50 kDa) mediante copolimerizzazione del substrato in gel di poliacrilamide (3). L'impiego di questa metodica ha permesso di visualizzare in Pneumocystis 6 bande ad attività proteasica. In particolare, é stato osservato che la banda 90 kDa viene inibita dall'EDTA, inibitore selettivo delle metalloproteasi. La purificazione di tale banda mediante cromatografia su HPT, suggerisce che questa proteasi é fortemente basica in quanto eluita da cationi bivalenti.
Ulteriori ricerche sono in corso per stabilire il ruolo di questa proteasi nella patogenesi dell'infezione.
Bibliografia essenziale
1- C. Contini, R. Romani, V. Vullo, S. Delia, F. Sorice.
Does laboratory recovery of Pneumocystis carinii always mean clinically significant disease? AIDS, 6, 1558-9, 1992
2- C. Contini, R. Romani, M. Manganaro, F. Sorice, S. Delia.
Tissue culture isolation of Pneumocystis carinii from peripheral blood mononuclear cells of AIDS patients with PCP.
AIDS, 1993, in press.
3- A. P. Massetti, F. Mengoni, C. Contini, G. Sebastiani, F. Folgori, V. Vullo, F. Sorice.
Characterization and partial purification of P. carinii proteases.
VIII International Conference on AIDS, 19-24 July 1992, PoB 3310
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