766 research outputs found
1915-10-14 Testimony of Mary Kinney
No full textThe testimony Mary Kinney given before the Carter County Circuit Court on October 14, 1915
1917-02-28 Testimony of Mary Mahala Kinney
No full textThe testimony of Mary Mahala Kinney given before the Lawrence County Circuit Court on February 28, 1917
Portrait of Mrs. Mary Strong Kinney
No full textPortrait of Mrs. Mary Strong Kinney, granddaughter of Pacific University and Tualatin Academy founder Tabitha Brown. She attended Willamette University and was a suffragette and Oregon State legislator.[back] Mrs. Mary Strong Kinney/great granddaughter [sic] of 'Grandma' T. Brown/students and alumni K -
Death of Stella Kinney
No full textA typescript document titled The Death of Stella Kinney, which was undated and no listed author
The marriage record of Kinney, Samuel and Williams, Mary
No full textMarriage license for Samuel Kinney and Mary Williams. George W. Larry was the officiant
Marriage record of Johnson, Elie and Kinney, Mary
No full textMarriage license for Elie Johnson and Mary Kinney. P.R. James was the officiant
Edwin Kinney Wright
No full textPhotograph - A portrait of Dr. Edwin Kinney Wright, Athabasca, Albert
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Prokaryotic Suppression Subtractive Hybridization Pcr Cdna Subtraction, A Targeted Method To Identity Diferentially Expressed Genes
No full textMolecular biology tools can be used to monitor and optimize biological treatment systems, but the application of nucleic acid-based tools has been hindered by the lack of available sequences for environmentally relevant biodegradation genes. The objective of our work was to extend an existing molecular method for eukaryotes to prokaryotes, allowing us to rapidly identify differentially expressed genes for subsequent sequencing. Suppression subtractive hybridization (SSH) PCR cDNA subtraction is a technique that can be used to identify genes that are expressed under specific conditions (e.g., growth on a given pollutant). While excellent methods for eukaryotic SSH PCR cDNA subtraction are available, to our knowledge, no methods previously existed for prokaryotes. This work describes our methodology for prokaryotic SSH PCR cDNA subtraction, which we validated using a model system: Pseudomonas putida mt-2 degrading toluene. cDNA from P. putida mt-2 grown on toluene (model pollutant) or acetate (control substrate) was subjected to our prokaryotic SSH PCR cDNA subtraction protocol to generate subtraction clone libraries. Over 90% of the sequenced clones contained gene fragments encoding toluene-related enzymes, and 20 distinct toluene-related genes from three key operons were sequenced. Based on these results, prokaryotic SSH PCR cDNA subtraction shows promise as a targeted method for gene identification.Gulf Coast Hazardous Waste Substances Research Center (043UTA0887)Strategic Environmental
Research and Development Program (W912 HQ-07-P-0039)Civil, Architectural, and Environmental Engineerin
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