3 research outputs found
Identification of novel additional uvrA genes in bacteria
No full textAmong all the pathways for removal of DNA damage, nucleotide excision repair (NER) is able to act on most types of DNA lesion. First component of NER is a DNA-binding protein UvrA. This protein allows the prokaryotes to identify DNA modifications, which are often varied in their chemical structure. Bacterial UvrA is a dimeric protein that belongs to the ATP-binding cassette (ABC) family of ATPases. The combination of UvrA with the other components of NER - UvrB, UvrC, and UvrD is essential to the survival of every living bacterium. Recently, it has been revealed that the chromosome in some prokaryotic organisms such as Xanthomonas axonopodis, Pseudomonas putida or Deinococcus radiodurans contains additional uvrA gene coding UvrA2 protein. There is no evidence that UvrA2 plays more important role in NER than UvrA but the deletion of uvrA2 gene had an effect on the UV-tolerance of bacteria only in the case when uvrA was also inactivated. We analyzed above 2400 bacterial genomes searching for additional uvrA genes and we have found many examples of bacteria which contain uvrA2 genes. The sequences of these genes vary in length - most of uvrA2 genes are coding proteins from 800 to 900 amino acids (like recently published Xanthomonas axonopodis 842 aa and Pseudomonas putida 838 aa) as UvrA proteins, however some UvrA2 are very huge (e. g. Acidovorax citrulli 1970 aa) which we discovered. There are also some bacteria that contain in their chromosome more than two uvrA genes, e.g Amycolatopsis mediterranei has three and Burkholderia phytofirmans has four different uvrA genes. Our studies suggest that we discovered new classes of UvrA proteins.status: Publishe
A new division of bacterial UvrA homologues
No full textThe UvrA protein is a DNA-binding and damage-recognition enzyme which participates in the prokaryotic type
nucleotide excision repair (NER) pathway. It has recently been noted that some bacterial genomes comprise
additional uvrA genes which encode five distinct types of UvrA homologue. We investigated the sequences of over
2400 bacterial genomes and found 130 examples of bacteria containing uvrA 2 genes. The sequence analyses
conducted on these UvrA homologues revealed that the previously established division of UvrA proteins might
be based on some incorrect assumptions. In this paper, we present the reasons for our creation of a new division
of UvrA homologues and a description of the four UvrA classes we have created.status: Publishe
