6 research outputs found
sj-pdf-1-vdi-10.1177_10406387211052966 – Supplemental material for Pandemic lineage 2009 H1N1 influenza A virus infection in farmed mink in Utah
No full textSupplemental material, sj-pdf-1-vdi-10.1177_10406387211052966 for Pandemic lineage 2009 H1N1 influenza A virus infection in farmed mink in Utah by Michael J. Clayton, E. Jane Kelly, Marta Mainenti, Amanda Wilhelm, Mia Kim Torchetti, Mary Lea Killian and Arnaud J. Van Wettere in Journal of Veterinary Diagnostic Investigation</p
Immunohistochemistry results (mean ± standard deviation) of Bcl-2, Bax, and Bad expression in luminal epithelial cells of canine mammary tumors.
No full textImmunohistochemistry results (mean ± standard deviation) of Bcl-2, Bax, and Bad expression in luminal epithelial cells of canine mammary tumors.</p
Signalment and morphological features of the canine mammary tumors included in the study.
No full textSignalment and morphological features of the canine mammary tumors included in the study.</p
Circulating cell-free (cf) DNA concentrations in healthy dogs and dogs with neoplastic and non-neoplastic diseases (diseased).
No full text(A) Scatterplot of cfDNA short and long fragments. (B) Box and Whiskers of cfDNA integrity index (long/short fragments) in healthy dogs and dogs with neoplastic and non-neoplastic diseases (diseased). (C) Box and Whiskers of cfDNA integrity index in neoplastic dogs with benign or malignant tumors and in healthy dogs. Error bars represent standard deviation. *P<0.05; ** P<0.01.</p
Photomicrographs of IHC in canine mammary gland. 1A-1F. IHC for Bcl-2, Bax, Bad.
No full text(A) Complex adenoma. Weak expression of Bcl-2 in benign tumors. (B) Simple tubulopapillary carcinoma, grade II. Strong expression of Bcl-2 in malignant tumors. (C) Intraductal papillary carcinoma, grade II. Weak expression of Bcl-2 in tumors with necrotic areas (arrow). (D) Complex carcinoma, grade II. Strong expression of Bcl-2 in tumors without necrosis. (E) Complex carcinoma, grade II. Higher expression of Bax in epithelial cells than in myoepithelial cells. (F) Complex carcinoma, grade I. Higher expression of Bad in epithelial cells than in myoepithelial cells.</p
Circulating Cell-Free DNA in Dogs with Mammary Tumors: Short and Long Fragments and Integrity Index
No full textCirculating cell-free DNA (cfDNA) has been considered an interesting diagnostic/prognostic plasma biomarker in tumor-bearing subjects. In cancer patients, cfDNA can hypothetically derive from tumor necrosis/apoptosis, lysed circulating cells, and some yet unrevealed mechanisms of active release. This study aimed to preliminarily analyze cfDNA in dogs with canine mammary tumors (CMTs). Forty-four neoplastic, 17 non-neoplastic disease-bearing, and 15 healthy dogs were recruited. Necrosis and apoptosis were also assessed as potential source of cfDNA on 78 CMTs diagnosed from the 44 dogs. The cfDNA fragments and integrity index significantly differentiated neoplastic versus non-neoplastic dogs (P<0.05), and allowed the distinction between benign and malignant lesions (P<0.05). Even if without statistical significance, the amount of cfDNA was also affected by tumor necrosis and correlated with tumor size and apoptotic markers expression. A significant (P<0.01) increase of Bcl-2 in malignant tumors was observed, and in metastatic CMTs the evasion of apoptosis was also suggested. This study, therefore, provides evidence that cfDNA could be a diagnostic marker in dogs carrying mammary nodules suggesting that its potential application in early diagnostic procedures should be further investigated
