4 research outputs found
Omalizumab treatment in Samter's triad: case series and review of the literature
No full textOBJECTIVE: Samter’s triad is the combination of asthma, aspirin sensitization, and nasal polyposis. Few data are available on the use of omalizumab in this disease. The study aimed to describe the impact of omalizumab on clinical and functional parameters and the quality of life of a series of patients with Samter’s triad. Moreover, we aimed to provide a review of the literature on this topic.
PATIENTS AND METHODS: We retrospectively described four patients with Samter’s triad undergoing omalizumab therapy. Clinical, functional, and immunological data of these patients were collected at baseline and follow-up.
RESULTS: Reduction of asthma exacerbations and salbutamol rescue therapy were observed in all patients after anti-IgE treatment together with an improvement in the quality of life. A significant improvement in FEV1, FVC, and FEF25-75 was observed. No major side-effects were observed. A total of 14 studies regarding omalizumab in aspirin-exacerbated respiratory diseases were included in the review, comprising 78 patients. All studies reported a good efficacy in improving asthma control; restoration of aspirin tolerance was repeatedly reported.
CONCLUSIONS: The results of our case series and review of the literature suggest that omalizumab effectively improves asthma control, lung function tests, and quality of life in patients with Samter’s triad
Immunomolecular and reactivity landscapes of gut IgA subclasses in homeostasis and inflammatory bowel disease
No full textThe human gut includes plasma cells (PCs) expressing immunoglobulin A1 (IgA1) or IgA2, two structurally distinct IgA subclasses with elusive regulation, function, and reactivity. We show here that intestinal IgA1+ and IgA2+ PCs co-emerged early in life, comparably accumulated somatic mutations, and were enriched within short-lived CD19+ and long-lived CD19- PC subsets, respectively. IgA2+ PCs were extensively clonally related to IgA1+ PCs and a subset of them presumably emerged from IgA1+ precursors. Of note, secretory IgA1 (SIgA1) and SIgA2 dually coated a large fraction of mucus-embedded bacteria, including Akkermansia muciniphila. Disruption of homeostasis by inflammatory bowel disease (IBD) was associated with an increase in actively proliferating IgA1+ plasmablasts, a depletion in long-lived IgA2+ PCs, and increased SIgA1+SIgA2+ gut microbiota. Such increase featured enhanced IgA1 reactivity to pathobionts, including Escherichia coli, combined with depletion of beneficial A. muciniphila. Thus, gut IgA1 and IgA2 emerge from clonally related PCs and show unique changes in both frequency and reactivity in IBD
Gut microbiota analysis in colombian adult population associated with risk factors of type 2 diabetes mellitus and obesity
No full textilustracionesLa microbiota intestinal es la comunidad de microorganismos vivos del tracto gastrointestinal, la pérdida del equilibrio en ésta se ha relacionado con diabetes mellitus tipo 2 (DM2) y obesidad; dos enfermedades de alta comorbilidad en las últimas décadas. El objetivo fue estudiar perfiles filogenéticos de la microbiota intestinal en adultos colombianos entre 40 y 70 años. Se realizaron jornadas de salud para el reclutamiento de voluntarios; se realizó valoración clínica a 535 participantes, que incluyó medidas de composición corporal y antropometría. Se aplicó la escala de riesgo FINDRISC y se extrajo muestra sanguínea para análisis bioquímicos; se recibió una muestra de materia fecal, para la extracción del ADN de la microbiota intestinal. El 70% de la población enrolada presentó alteración en el índice de masa corporal (IMC), el 50% riesgo a desarrollar DM2 y el 66% presentó riesgo metabólico.
Se seleccionaron 85 voluntarios para el estudio de microbiota intestinal, mediante secuenciación del gen 16S rRNA, utilizando la plataforma Ion Torrent de ThermoFisher. Los análisis bioinformáticos se realizaron sobre los resultados de 40 muestras discriminadas según IMC y el comportamiento glucémico, utilizando el Software QIIME 2TM, los análisis de biodiversidad y de biomarcadores microbianos se realizaron en el Software MicrobiomeAnalyst. Los filos bacterianos más abundantes fueron Firmicutes (49%), Bacteroidetes (39%) y Proteobacteria (7%); se observó que predominaron taxones microbianos asociados a un patrón de alimentación occidental. Se logró identificar catorce biomarcadores microbianos diferentes en cada subgrupo; se encontró relación estadística significativa entre familias, géneros y especies con diferentes parámetros clínicos asociados a disbiosis y de riesgo metabólico. (Texto tomado de la fuente)The gut microbiota is the community of living microorganisms of the gastrointestinal tract,
the loss of balance in it has been related to type 2 diabetes mellitus (DM2) and obesity; two
diseases with high comorbidity in recent decades. The objective was to study phylogenetic
profiles of the intestinal microbiota in Colombian adults between 40 and 70 years old.
Health sessions were held to recruit volunteers; A clinical assessment was performed on
535 participants, which included measurements of body composition and anthropometry.
The FINDRISC risk scale was applied, and a blood sample was taken for biochemical
analysis; a stool sample was received for DNA extraction from the intestinal microbiota.
70% of the enrolled population presented an alteration in the body mass index (BMI), 50%
risk of developing DM2 and 66% presented metabolic risk.
85 volunteers were selected for the intestinal microbiota study, by sequencing the 16S
rRNA gene, using ThermoFisher's Ion Torrent platform. The bioinformatic analyzes were
carried out on the results of 40 samples discriminated according to BMI and glycemic
behavior, using the QIIME 2TM Software, the biodiversity and microbial biomarker analyzes
were carried out in the MicrobiomeAnalyst Software. The most abundant bacterial phyla
were Firmicutes (49%), Bacteroidetes (39%) and Proteobacteria (7%); it was observed that
microbial taxa associated with a western feeding pattern predominated. Fourteen different
microbial biomarkers were identified in each subgroup; a significant statistical relationship
was found between families, genera and species with different clinical parameters
associated with dysbiosis and metabolic risk.MaestríaMagíster en Ciencias - Microbiologí
