1,721,145 research outputs found
A novel nested PCR for the diagnosis of calicivirus infections in the cat
No full textA novel nested PCR (nPCR) assay is reported on the diagnosis of the feline calicivirus (FCV) infection. The test was performed on 47 ocular and 40 pharyngeal swabs collected from 47 cats with respiratory syndrome; among the 87 samples examined, 18 ocular and 23 pharyngeal swabs were positive in nPCR. The nPCR sensitivity was compared to other diagnostic techniques such as virus isolation on cell culture and reverse transcriptase-polymerase chain reaction (RT-PCR). The nPCR was more sensitive than the virus isolation and RT-PCR; therefore, it can be used for calicivirosis diagnosis in cats
Ulteriori prove di infezione sperimentale del gatto con il virus dell'immunodeficienza felina (FIV)
No full textRicerche sulla presenza di virus influenzali e relativi anticorpi in cavalli importati dall'estero a scopo di macellazione
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Aminoacid esters and imidazole derivatives of polyphosphazenes: influence in the release of drugs, degradability and swelling
No full textAminoacid esters and imidazole derivatives of polyphosphazenes: influence in the release of drugs, degradability and swellin
Preparation and characterisation of polyphosphazene-based controlled release systems for naproxen
No full textA new polyphosphazene polymer suitable for preparation of naproxen controlled release systems has been prepared by non complete substitution of the chlorine at the phosphorus atoms of polydichlorophosphazene with phenylalanine ethyl ester and imidazole. Polymeric disks obtained by solvent evaporation were found to maintain their integrity for months either "in vivo" and "in vitro" after incubation in buffer, although the intrinsic viscosity undergoes a rapid decrease. The release rate of naproxen from the films was found to depend on thickness of the matrixes and amount of entrapped drug. "In vitro" release spikes, corresponding to initial in plasma burst after subcutaneous implantation of the films in rats, have been observed with high concentrations of drug in the matrixes. On the other end decreasing the drug concentrations in the polymer matrixes, the naproxen release approximates a zero order kinetic and a controlled release is obtained for weeks. Scanning electron microscopy showed that the disks giving spikes in drug release are characterised by the presence of naproxen crystals on the matrix surface. "In vivo" studies are demonstrating that the initial naproxen concentration spike is useful in the treatment of acute models of inflammation, while a more constant and lasting release is successful in chronic inflammation models. The degradation profile of this new polyphosphazene as well as preliminary pharmacological data are also reported
Nota preliminare sulla ricerca di Rotavirus nelle feci di agnelli e capretti a mezzo della PAGE
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