94 research outputs found

    (Anura: Dicroglossidae)

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    Figure 2. Head of a male Limnonectes gyldenstolpei, mediosagittally split. The actual specimen is shown on the left, whereas the right-hand image shows the H&E stained surface of the corresponding view. Note the flap-like caudad curve of the posterior margin of the caruncle (ca). Abbreviations: br, brain; ca, caruncle; to, tongue. Scale bar: 1 cm.Published as part of Lambertz, Markus, Hartmann, Timo, Walsh, Shannon, Geissler, Peter & Mcleod, David S., 2014, Anatomy, histology, and systematic implications of the head ornamentation in the males of four species of Limnonectes (Anura: Dicroglossidae), pp. 117-132 in Zoological Journal of the Linnean Society 172 (1) on page 121, DOI: 10.1111/zoj.12171, http://zenodo.org/record/531395

    Response to:Phylogenetic placement, developmental trajectories and evolutionary implications of a feathered dinosaur tail in Mid-Cretaceous amber

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    In his correspondence, Markus Lambertz [1] raises some concerns about the phylogenetic placement and feather development of DIP-V-15103, the amber-entombed tail section that we recently reported [2] as fragmentary remains of a non-pygostylian coelurosaur (likely within the basal part of Coelurosauria). We here would like to respond to these concerns.</p

    Two Fatty Acid Desaturases, STEAROYL-ACYL CARRIER PROTEIN Delta(9)-DESATURASE6 and FATTY ACID DESATURASE3, Are Involved in Drought and Hypoxia Stress Signaling in Arabidopsis Crown Galls

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    Agrobacterium tumefaciens-derived crown galls of Arabidopsis (Arabidopsis thaliana) contain elevated levels of unsaturated fatty acids and strongly express two fatty acid desaturase genes, omega 3 FATTY ACID DESATURASE3 (FAD3) and STEAROYL-ACYL CARRIER PROTEIN Delta(9)-DESATURASE6 (SAD6). The fad3-2 mutant with impaired alpha-linolenic acid synthesis developed significantly smaller crown galls under normal, but not under high, relative humidity. This strongly suggests that FAD3 plays a role in increasing drought stress tolerance of crown galls. SAD6 is a member of the SAD family of as yet unknown function. Expression of the SAD6 gene is limited to hypoxia, a physiological condition found in crown galls. As no sad6 mutant exists and to link the function of SAD6 with fatty acid desaturation in crown galls, the lipid pattern was analyzed of plants with constitutive SAD6 overexpression (SAD6-OE). SAD6-OE plants contained lower stearic acid and higher oleic acid levels, which upon reduction of SAD6 overexpression by RNA interference (SAD6-OE-RNAi) regained wild-type-like levels. The development of crown galls was not affected either in SAD6-OE or SAD6-OE-RNAi or by RNA interference in crown galls. Since biochemical analysis of SAD6 in yeast (Saccharomyces cerevisiae) and Escherichia coli failed, SAD6 was ectopically expressed in the background of the well-known suppressor of salicylic acid-insensitive2 (ssi2-2) mutant to confirm the desaturase function of SAD6. All known ssi2-2 phenotypes were rescued, including the high stearic acid level. Thus, our findings suggest that SAD6 functions as a Delta(9)-desaturase, and together with FAD3 it increases the levels of unsaturated fatty acids in crown galls under hypoxia and drought stress conditions.Deutsche Forschungsgemeinschaft [GRK1342 [TP A7], SFB 567 [TP B5]

    Taxonomy: retain scientific autonomy

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