1,444 research outputs found
Disruption of the developmental programme of Trypanosoma brucei by genetic ablation of TbZFP1, a differentiation-enriched CCCH protein
No full textThe regulation of differentiation is particularly important in microbial eukaryotes that inhabit multiple environments. The parasite Trypanosoma brucei is an extreme example of this, requiring exquisite gene regulation during transmission from mammals to the tsetse fly vector. Unusually, trypanosomes rely almost exclusively on post-transcriptional mechanisms for regulated gene expression. Hence, RNA binding proteins are potentially of great significance in controlling stage-regulated processes. We have previously identified TbZFP1 as a trypanosome molecule transiently enriched during differentiation to tsetse midgut procyclic forms. This small protein (101 amino acids) contains the unusual CCCH zinc finger, an RNA binding motif. Here, we show that genetic ablation of TbZFP1 compromises repositioning of the mitochondrial genome, a specific event in the strictly regulated differentiation programme. Despite this, other events that occur both before and after this remain intact. Significantly, this phenotype correlates with the TbZFP1 expression profile during differentiation. This is the first genetic disruption of a developmental regulator in T. brucei. It demonstrates that programmed events in parasite development can be uncoupled at the molecular level. It also further supports the importance of CCCH proteins in key aspects of trypanosome cell function
Correction to: Assessing the quality of cardiac rehabilitation programs by measuring adherence to the Australian quality indicators
No full textFollowing publication of the original article [1], the authors identified two errors in the author names. Firstly, the two co-authors’ names J Hendriks and C Gallagher were mistakenly merged into one name ‘Hendriks Gallagher JC’. Secondly, the given name and family name were erroneously transposed. The author group has been updated above and the original article [1] has been corrected
In search of the original text in Mark 9:38
No full textThe text-critical situation of Mark 9:38 is very complicated. This complex situation already becomes evident in the different readings of the critical editions. While there is almost no variation in the first part of the verse, the critical editions offer four different readings in the last part of it. So there is until now no consensus at all. The question remains which text appears to be the more original one. Several scholars – such as Vaganay, Duplacy, Amphoux, and others – in search of the original text of Mark – opt for a so called Western text, the main stream still prefers the Neutral text of Westcott and Hort. A text-critical analysis of Mark 9:38 may provide an answer to this question
The Impact of Phytosterols on the Healthy and Diseased Brain
No full textThe central nervous system (CNS) is the most cholesterol-rich organ in mammals. Cholesterol homeostasis is essential for proper brain functioning and dysregulation of cholesterol metabolism can lead to neurological problems. Multiple sclerosis (MS) and Alzheimer's disease (AD) are examples of neurological diseases that are characterized by a disturbed cholesterol metabolism. Phytosterols (PS) are plant-derived components that structurally and functionally resemble cholesterol. PS are known for their cholesterol-lowering properties. Due to their ability to reach the brain, researchers have started to investigate the physiological role of PS in the CNS. In this review, the metabolism and function of PS in the diseased and healthy CNS are discussed.This work was supported by grants of the Research Foundation Flanders (FWO; 12J9116N, 12JG119N, and G099618N to Tess Dierckx, Jeroen F.J. Bogie and Jerome J.A. Hendriks).Hendriks, JJA (reprint author), Hasselt Univ, Biomed Res Inst, Diepenbeek, Hassett, Belgium.
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Cytochrome oxidase subunit VI of Trypanosoma brucei is imported without a cleaved presequence and is developmentally regulated at both RNA and protein levels
No full textMitochondrial respiration in the African trypanosome undergoes dramatic developmental stage regulation. This requires co-ordinated control of components encoded by both the nuclear genome and the kinetoplast, the unusual mitochondrial genome of these parasites. As a model for understanding the co-ordination of these genomes, we have examined the regulation and mitochondrial import of a nuclear-encoded component of the cytochrome oxidase complex, cytochrome oxidase subunit VI (COXVI). By generating transgenic trypanosomes expressing intact or mutant forms of this protein, we demonstrate that COXVI is not imported using a conventional cleaved presequence and show that sequences at the N-terminus of the protein are necessary for correct mitochondrial sorting. Analyses of endogenous and transgenic COXVI mRNA and protein expression in parasites undergoing developmental stage differentiation demonstrates a temporal order of control involving regulation in the abundance of, first, mRNA and then protein. This represents the first dissection of the regulation and import of a nuclear-encoded protein into the cytochrome oxidase complex in these organisms, which were among the earliest eukaryotes to possess a mitochondrion
In search of the original text in Mark 9:38
No full textThe text-critical situation of Mark 9:38 is very complicated. This complex situation already becomes evident in the different readings of the critical editions. While there is almost no variation in the first part of the verse, the critical editions offer four different readings in the last part of it. So there is until now no consensus at all. The question remains which text appears to be the more original one. Several scholars – such as Vaganay, Duplacy, Amphoux, and others – in search of the original text of Mark – opt for a so called Western text, the main stream still prefers the Neutral text of Westcott and Hort. A text-critical analysis of Mark 9:38 may provide an answer to this question
In search of the original text in Mark 9:38
No full textThe text-critical situation of Mark 9:38 is very complicated. This complex situation already becomes evident in the different readings of the critical editions. While there is almost no variation in the first part of the verse, the critical editions offer four different readings in the last part of it. So there is until now no consensus at all. The question remains which text appears to be the more original one. Several scholars – such as Vaganay, Duplacy, Amphoux, and others – in search of the original text of Mark – opt for a so called Western text, the main stream still prefers the Neutral text of Westcott and Hort. A text-critical analysis of Mark 9:38 may provide an answer to this question.Publisher's versio
Improving the experience of patients living with an implantable cardioverter defibrillator
No full textThis Science for Patients issue describes the experience of patients who have an implantable cardioverter defibrillator (ICD) fitted and what they and their families and carers should expect from their health care professionals to improve this experience.
The researchers looked for studies published since 2005 that reported on patients’ stories and feelings about the experience of having an ICD and what they expect from their health care.
The researchers included 24 studies all of which used a ‘qualitative’ research methodology. This means that they collected and analysed non-numerical data to understand the experiences and emotions of the patients included in the studies. This allowed an in-depth insight into the issues in questio
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