349 research outputs found
sj-pdf-3-jcb-10.1177_0271678X231209641 - Supplemental material for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI
No full textSupplemental material, sj-pdf-3-jcb-10.1177_0271678X231209641 for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI by Phoebe G Evans, Maria Sajic, Yichao Yu, Ian F Harrison, Patrick S Hosford, Ken J Smith, Mark F Lythgoe, Daniel J Stuckey and Jack A Wells: on behalf of the CONTRAST consortium in Journal of Cerebral Blood Flow & Metabolism</p
sj-pdf-2-jcb-10.1177_0271678X231209641 - Supplemental material for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI
No full textSupplemental material, sj-pdf-2-jcb-10.1177_0271678X231209641 for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI by Phoebe G Evans, Maria Sajic, Yichao Yu, Ian F Harrison, Patrick S Hosford, Ken J Smith, Mark F Lythgoe, Daniel J Stuckey and Jack A Wells: on behalf of the CONTRAST consortium in Journal of Cerebral Blood Flow & Metabolism</p
sj-pdf-4-jcb-10.1177_0271678X231209641 - Supplemental material for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI
No full textSupplemental material, sj-pdf-4-jcb-10.1177_0271678X231209641 for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI by Phoebe G Evans, Maria Sajic, Yichao Yu, Ian F Harrison, Patrick S Hosford, Ken J Smith, Mark F Lythgoe, Daniel J Stuckey and Jack A Wells: on behalf of the CONTRAST consortium in Journal of Cerebral Blood Flow & Metabolism</p
sj-pdf-1-jcb-10.1177_0271678X231209641 - Supplemental material for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI
No full textSupplemental material, sj-pdf-1-jcb-10.1177_0271678X231209641 for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI by Phoebe G Evans, Maria Sajic, Yichao Yu, Ian F Harrison, Patrick S Hosford, Ken J Smith, Mark F Lythgoe, Daniel J Stuckey and Jack A Wells: on behalf of the CONTRAST consortium in Journal of Cerebral Blood Flow & Metabolism</p
sj-pdf-5-jcb-10.1177_0271678X231209641 - Supplemental material for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI
No full textSupplemental material, sj-pdf-5-jcb-10.1177_0271678X231209641 for Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI by Phoebe G Evans, Maria Sajic, Yichao Yu, Ian F Harrison, Patrick S Hosford, Ken J Smith, Mark F Lythgoe, Daniel J Stuckey and Jack A Wells: on behalf of the CONTRAST consortium in Journal of Cerebral Blood Flow & Metabolism</p
Acupuncture needling sensation: the neural correlates of deqi using fMRI
No full textThe needling sensation of deqi is considered by most acupuncturists to be an important component of acupuncture, yet neuroimaging research that investigates this needle sensation has been limited. In this study we have investigated the effect of deqi and acute pain needling sensations upon brain fMRI blood oxygen level-dependent (BOLD) signals. Seventeen right-handed participants who received acupuncture at the right LI-4 (Hegu) acupoint were imaged in a 3T MRI scanner. fMRI datasets were classified, on the basis of psychophysical participants' reports of needling scores, into those that were associated with predominantly deqi sensations versus those with predominantly acute pain sensations. Brain areas showing changes in BOLD signal increases (activations) and decreases (deactivations) were identified. Differences were demonstrated in the pattern of activations and deactivations between groupings of scans associated with deqi versus pain sensations. For the deqi grouping, significant deactivations occurred, whereas significant activations did not. In contrast, the predominantly acute pain grouping was associated with a mixture of activations and deactivations. For the comparison between the predominately deqi sensation grouping and the acute pain sensation grouping (deqi>pain contrast), only negative Z value voxels resulted (mainly from deactivations in the deqi grouping and activations in the pain grouping) in the limbic/sub-cortical structures and the cerebellum regions of interest. Our results show the importance of collecting and accounting for needle sensation data in neuroimaging studies of acupunctur
Amniotic fluid stem cells prevent development of ascites in a neonatal rat model of necrotizing enterocolitis.
No full textIt has been demonstrated that in a neonatal rat model of necrotizing enterocolitis (NEC), amniotic fluid stem (AFS) cells decrease intestinal damage and improve survival via modulation of stromal cells expressing cyclooxygenase 2 in the lamina propria. Herein, we aimed to evaluate the effect of AFS cells on body weight and fluid retention in this NEC model. Methods AFS cells were obtained from green fluorescent protein (GFP) + pregnant rats at E16 and expanded in culture. A total of 185 neonatal rats had NEC induced by gavage feeding of hypertonic formula + hypoxia + oral lipopolysaccharide (4 mg/kg/d) and were randomized to intraperitoneal phosphate buffered saline (PBS, n = 93) or AFS cells (n = 92). A total of 36 breastfed (BF) rats were used as controls. All rats were being killed at 96 hours of life. Groups were compared for body weight and presence of free intraperitoneal fluid using nonparametric and contingency tests. Data are expressed as mean ± standard deviation. There were no differences in birth weight among the groups (PBS = 5.6 ± 0. 3 g; AFS cells = 5.6 ± 0. 3 g; BF = 5.6 ± 0. 3 g; p = 1). The body weight at randomization was not different between PBS (5.61 ± 0. 5 g) and AFS cells (5.60 ± 0. 5; p = 1) rats. After the rats were killed, BF rats were significantly heavier (12.5 ± 0.1 g) than PBS (5.12 ± 0.4 g) and AFS cell rats (4.95 ± 0.3; p < 0.0001). From randomization to being killed, PBS rats had 9% of weight loss in comparison with 12% in AFS cell rats (p = 0.08). After the rats were killed, 42 (45%) PBS rats developed ascites with evident abdominal distension in comparison with 19 (21%) AFS cells (p = 0.0005). None of BF animals had ascites. Gavage feeding and undernutrition severely affect growth in this model of NEC. Administration of AFS cells result in lower incidence of ascites than in PBS rats. This could explain the differences in body weight between the two groups of rats that were killed. Furthermore, studies on liver function and fluid composition are needed to investigate our speculation. Georg Thieme Verlag KG Stuttgart · New York
Brain imaging of acupuncture: comparing superficial with deep needling
No full textThe difference between superficial and deep needling at acupuncture points has yet to be mapped with functional magnetic resonance imaging (fMRI). Using a 3 T MRI, echo planar imaging data were acquired for 17 right-handed healthy volunteer participants. Two fMRI scans of acupuncture needling were taken in random order in a block design, one for superficial and one for deep needling on the right hand at the acupuncture point LI-4 (Hegu), with the participant blind to the order. For both scans needle stimulation was used. Brain image analysis tools were used to explore within-group and between-group differences in the blood oxygen level dependent (BOLD) responses. The study demonstrated marked similarities in BOLD signal responses between superficial and deep needling, with no significant differences in either activations (increases in BOLD signal) or deactivations (decreases in BOLD signal) above the voxel Z score of 2.3 with corrected cluster significance of P = 0.05. For both types of needling, deactivations predominatid over activations. These fMRI data suggest that acupuncture needle stimulation at two different depths of needling, superficial and deep, do not elicit significantly different BOLD responses. This data is consistent with the equivalent therapeutic outcomes that are claimed by proponents of Japanese and Chinese styles of acupuncture that utilise superficial and deep needling, respectively
Studies of experimental cerebral ischaemia using magnetic resonance imaging and autoradiography
No full textSIGLEAvailable from British Library Document Supply Centre-DSC:DXN029977 / BLDSC - British Library Document Supply CentreGBUnited Kingdo
Multifunctional receptor-targeted nanocomplexes for magnetic resonance imaging and transfection of tumours.
No full textThe efficient targeted delivery of nucleic acids in vivo provides some of the greatest challenges to the development of genetic therapies. We aim to develop nanocomplex formulations that achieve targeted transfection of neuroblastoma tumours that can be monitored simultaneously by MRI. Here, we have compared nanocomplexes comprising self-assembling mixtures of liposomes, plasmid DNA and one of three different peptide ligands derived from ApoE, neurotensin and tetanus toxin for targeted transfection in vitro and in vivo. Neurotensin-targeted nanocomplexes produced the highest levels of transfection and showed a 4.7-fold increase in transfected luciferase expression over non-targeted nanocomplexes in Neuro-2A cells. Transfection of subcutaneous Neuro-2A tumours in vivo with neurotensin-targeted nanocomplexes produced a 9.3-fold increase in gene expression over non-targeted controls. Confocal microscopy analysis elucidated the time course of DNA delivery with fluorescently labelled nanocomplex formulations in cells. It was confirmed that addition of a gadolinium lipid conjugate contrast agent allowed real time in vivo monitoring of nanocomplex localisation in tumours by MRI, which was maintained for at least 24 h. The peptide-targeted nanocomplexes developed here allow for the specific enhancement of targeted gene therapy both in vitro and in vivo, whilst allowing real time monitoring of delivery with MRI
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