3 research outputs found
Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study
No full textSurfaces of implanted medical devices are highly susceptible to biofilm formation. Bacteria in biofilms are embedded in a self-produced extracellular matrix that inhibits the penetration of antibiotics and significantly contributes to the mechanical stability of the colonizing community which leads to an increase in morbidity and mortality rate in clinical settings. Therefore, new antibiofilm approaches and substances are urgently needed. In this paper, we test the efficacy of a broad-range recombinant endolysin of the coliphage LysECD7 against forming and mature biofilms. We used a strong biofilm producer—Klebsiella pneumoniae Ts 141-14 clinical isolate. In vitro investigation of the antibacterial activity was performed using the standard biofilm assay in microtiter plates. We optimized the implantable diffusion chamber approach in order to reach strong biofilm formation in vivo avoiding severe consequences of the pathogen for the animals and to obtain a well-reproducible model of implant-associated infection. Endolysin LysECD7 significantly reduced the biofilm formation and was capable of degrading the preformed biofilm in vitro. The animal trials on the preformed biofilms confirmed these results. Overall, our results show that LysECD7 is a promising substance against clinically relevant biofilms
Neighborhood Environment and Stress: Findings from the ESSE-RF-3 Epidemiological Study
No full textIntroduction — Both Russian and international studies demonstrate that living environment and neighborhood characteristics have influence on health status, including mental well-being. The aim of the study was to examine the associations between neighborhood environment and individual likelihood of stress among the Russian population.
Material and Methods — The multicentre study “Epidemiology of Cardiovascular Diseases and Their Risk Factors in the Regions of the Russian Federation. Third Survey” was conducted in 2020-2022 across 15 regions of Russia. The analysis included 28,731 men and women aged 35-74 years. Neighborhood infrastructure was assessed using the Russian version of the NEWS questionnaire, while stress was measured with the Perceived Stress Scale (PSS; Cohen’s Scale). Associations between stress and neighborhood infrastructure characteristics were evaluated using logistic regression adjusted for covariates. Results were presented as odds ratios (OR) with 95% confidence intervals (CI).
Results — A lower likelihood of stress was associated with shorter distances from the place of residence to facilities (OR=0.91; 95% CI 0.87-0.94), greater accessibility of key facilities (OR=0.51; 95% CI 0.48-0.55), and improved traffic-related safety (OR=0.60; 95% CI 0.56-0.66). In contrast, higher population density (OR=1.002; 95% CI 1.001-1.004), better pedestrian infrastructure quality (OR=1.16; 95% CI 1.10-1.22), and enhanced neighborhood aesthetics (OR=1.08; 95% CI 1.03-1.13) were associated with increased stress probability.
Conclusion — The results of the study showed a substantial impact of certain neighborhood characteristics on stress, an indicator of mental health in the Russian population. The link between residential environment and mental health can be applicable in public health, policy-making, and urban planning
Abstracts of the First Eurasian Conference; The Coronavirus Pandemic and Critical ICT Infrastructure
Get PDFWhile the world is struggling with COVID-19, the ICT industry seeks to play a constructive role in combating the spread of the virus. This book contains the abstracts of the papers presented at The First Eurasian Conference; The Coronavirus Pandemic and Critical ICT Infrastructure (PANDEMIC-ICT-2020) organized by AMIR Technical Services LLC, Tbilisi, Georgia held on November 28-30, 2020.
Conference Title: The Coronavirus Pandemic and Critical ICT InfrastructureConference Acronym: PANDEMIC-ICT-2020Conference Date: 28-30 November 2020Conference Location: Online (Virtual Mode)Conference Organizer: AMIR Technical Services LLC, Tbilisi, Georgi
