130,911 research outputs found
Identification of a TGF-beta responsive element in the human elastin promoter
No full textIn a previous report (Marigo, V., Volpin, D., and Bressan, G. M. (1993) Biochim. Biophys. Acta 1172, 31-36) it was shown that the elastin promoter contains a region mediating transcriptional activation by TGF-β in aorta cells, but not in tendon fibroblasts from chick embryos. In this paper we have identified the sequence responsible for this effect by a combination of CAT assays with mutant constructs, DNase I footprinting and electrophoretic mobility shift assays. This TGF-β responsive element binds different nuclear proteins in chick embryo aorta and tendon cells. Whereas association of the aorta protein(s) to the element is necessary for TGF-β activation, binding of the tendon protein(s) has apparently no effect on promoter stimulation by the cytokine
A natureza de Luiz Claudio Marigo
No full textO filme A Natureza de Luiz Claudio Marigo conta a trajetória de Luiz Claudio Marigo, considerado um dos brasileiros pioneiros na fotografia de natureza e autor da fotografia do último indivíduo de ararinha-azul livre remanescente. A obra, realizada no formato de documentário, mostra aspectos tanto da carreira profissional quanto pessoais do fotógrafo a partir do depoimento de especialistas, familiares e amigos. Marigo revelou-se um grande ambientalista ao utilizar a fotografia como instrumento para propagar a beleza da natureza e despertar o interesse da população pelo conhecimento das espécies e, consequentemente, sua preservação. Para compor a narrativa do filme, fotografias de Marigo são mescladas entre as entrevistas, que destacam trabalhos como as fotos que fez para o Chocolate Surpresa da Nestlé – e influenciaram diversas pessoas de uma geração a seguirem carreiras ligadas à biologia, e a criação da Reserva de Desenvolvimento Sustentável do Mamirauá
Exposición que ante la Suprema Corte de Justicia de la Nación hace Isaías Marigo : en el juicio de amparo promovido por la parte de d. Enrique Hillebrand : contra el auto consentido que manda ejecutar el laudo del árbitro-arbitrador don Martin Castillo en el juicio que sobre cumplimiento de contracto siguió Marigo contra dicho sr. Hillebrand en el juzgado 2o. de lo civil.
No full textSigned (p. 31): I. Marigo, Justo Benítez.Mode of access: Internet
Identification of a TGF-beta responsive element in the human elastin promoter.
No full textIn a previous report (Marigo, V., Volpin, D., and Bressan, G. M. (1993) Biochim. Biophys. Acta 1172, 31-36) it was shown that the elastin promoter contains a region mediating transcriptional activation by TGF-beta in aorta cells, but not in tendon fibroblasts from chick embryos. In this paper we have identified the sequence responsible for this effect by a combination of CAT assays with mutant constructs, DNase I footprinting and electrophoretic mobility shift assays. This TGF-beta responsive element binds different nuclear proteins in chick embryo aorta and tendon cells. Whereas association of the aorta protein(s) to the element is necessary for TGF-beta activation, binding of the tendon protein(s) has apparently no effect on promoter stimulation by the cytokine
Cross-talk between two apoptotic pathways activated by endoplasmic reticulum stress: differential contribution of caspase-12 and AIF
No full textCo-activation and cross-talk of different apoptotic pathways have been described in several systems however, the differential contributions of the different executors have not been well characterized. Here we report the co-translocation to the nucleus of caspase-12 and AIF in response to two endoplasmic reticulum (ER) stresses: protein misfolding and disruption of calcium homeostasis. As seen by treatment with pan-caspase inhibitor and calpain inhibitors, apoptosis is not mediated by executor caspases but by calpains. By reduction of AIF or caspase-12 expression we unraveled that AIF primarily controls apoptosis caused by changes in calcium homeostasis while caspase-12 has a main role in programmed cell death induced by protein misfolding. Nevertheless, the two apoptotic factors appear to reinforce each other during the apoptotic process, confirming that while the first response primarily involves one organelle, mitochondria and ER can influence each other in the apoptotic event
Asymptotic giant branch evolution: Where do we stand?
No full textI provide a synthetic overview of the present status of stellar models for the asymptotic giant branch phase, one of the most complex and still uncertain stages of stellar evolution. In particular I will focus on two aspects that are most relevant in the context of the planetary nebulæ progeny, namely: the chemical composition of the AGB ejecta, and the mass of the bare CO core left after the ejection of the stellar mantle at the AGB tip. Recent progress, present uncertainties, and future perspectives to constrain AGB models are briefly discussed
Equivalence relations and operators on ordered algebraic structures with difference.
No full textThis work concerns algebraic models of fuzzy and many-valued propositional logics, in particular Boolean Algebras, Heyting algebras, GBL-algebras and their dual structures, and partial algebras.
The central idea is the representation of complex structures through simpler structures and equivalence relations on them: in order to achieve this, a structure is often considered under two points of view, as total algebra and partial algebra. The equivalence relations which allow the representation are congruences of partial algebras.
The first chapter introduces D-posets, the partial algebraic structures used for this representation, which generalize Boolean algebras and MV-algebras.
The second chapter is a study of congruences on D-posets and the structure of the quotients, in particular for congruences induced by some kinds of idempotent operators, here called S-operators. The case of Boolean algebras and MV-algebras is studied more in detail.
The third chapter introduces GBL-algebras and their dual, and shows how the interplay of an S-operator with a closure operator gives rise to a dual GBL-algebra. Other results about the representation of finite GBL-algebras and GBL*algebras (GBL-algebras with monoidal sum), part of two papers previously published, are summarized and put in relation with the other results of this work
Rolling polyhedra on a plane, analysis of the reachable set
No full textThe problem of dexterous manipulation of objects, i.e. of arbitrary relocation and reorientation of rigid bodies by action of some mechanism, is considered. We build upon previous results on the possibility of implementing dexterous “robot hands” with few actuators, which can be afforded through the exploitation of nonholonomic rolling of regular surfaces. In this paper we focus on the manipulation of polyhedral objects, and prove a necessary and sufficient controllability–like result, which discloses some of the interesting aspects and perspectives of this problem
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